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3 Biotech

, 8:392 | Cite as

Fe(III)-based immobilized metal–affinity chromatography (IMAC) method for the separation of the catechol siderophore from Bacillus tequilensis CD36

  • Yunya Li
  • Wei Jiang
  • Ruijie Gao
  • Yujie Cai
  • Zhengbing Guan
  • Xiangru Liao
Short Reports
  • 68 Downloads

Abstract

Catechol siderophore plays an important role in microbial ecology, agriculture, and medicine, but its research is often limited by the difficulty in acquisition of it in large quantities. Based on evidence from the coordination chemistry and chemical biology, catechol siderophore could chelate Fe3+ with high affinity. Therefore, Fe(III)-based immobilized metal–affinity chromatography (IMAC) was applied to capture siderophore from the culture filtrate of Bacillus tequilensis CD36. The ethanol-precipitated sample and the separated sample from Fe(III)-based IMAC were analyzed by liquid chromatography–mass spectrometry. According to the result, the pure siderophore DHB-Gly-Thr could be extracted from the ethanol-precipitated sample. Compared with other purifications, Fe(III)-based IMAC was convenient and had fewer steps. In addition, it also reduced the use of toxic chemical solvents in some traditional extraction process, such as extraction and ion exchange chromatography. Fe(III)-based IMAC was successfully used in separation of the catechol siderophore from B. tequilensis CD36. The results revealed that Fe(III)-based IMAC was an efficient and environmentally friendly method for the separation and purification of catechol siderophore.

Keywords

Fe(III)-based IMAC Catechol siderophore LC–MS Bacillus tequilensis 

Notes

Acknowledgements

This work was financially supported by the Collaborative Innovation Involving Production, Teaching and Research Funds of Jiangsu Province (BY2014023-28) and the Agricultural Support Project, Wuxi Science and Technology Development (CLE01N1310). This research was also funded by the Program of Marine Biological Resources Exploitation and Utilization of Science and Technology Innovation Team of Taizhou (no. 1302ky08).

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Yunya Li
    • 1
  • Wei Jiang
    • 1
  • Ruijie Gao
    • 1
  • Yujie Cai
    • 1
  • Zhengbing Guan
    • 1
  • Xiangru Liao
    • 1
  1. 1.Key Laboratory of Industrial Biotechnology, Ministry of Education, School of BiotechnologyJiangnan UniversityWuxiChina

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