Degradation of cinnamic acid by a newly isolated bacterium Stenotrophomonas sp. TRMK2
A bacterium Stenotrophomonas sp. TRMK2 capable of utilizing cinnamic acid was isolated from agro-industrial waste by enrichment culture technique. This strain completely utilizes 5 mM cinnamic acid within 18 h of incubation. The different metabolites formed during the degradation of cinnamic acid were characterized by GC-HRMS. The involvement of various enzymes, namely cinnamate reductase, 3-phenylpropionic acid hydroxylase, p-hydroxybenzoic acid hydroxylase and protocatechuate 3,4-dioxygenase in cinnamic acid degradation was demonstrated. A catabolic pathway for cinnamic acid in Stenotrophomonas sp. TRMK2 is as follows: Cinnamic acid; 3-Phenylpropionic acid; 3-(4-Hydroxyphenyl) propionic acid; 4-Hydroxy benzoic acid and Protocatechuic acid. Further, this strain is capable of utilizing various phenolic compounds.
KeywordsBiodegradation Enzymes Metabolism Pollutants Waste
One of the authors Mr. Monisha T. R. would like to thank University Grants Commission (UGC), New Delhi, India for the financial assistance in the mode of BSR Fellowship. Dr. T. B Karegoudar is grateful to UGC for awarding UGC-BSR faculty fellowship. The financial support from UGC in the form of UGC-SAP programme [No.F.4–27/2015/DRS-II (SAP-II)] sanctioned to the department is highly acknowledged.
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Conflict of interest
Authors declare that the manuscript does not contain any conflict of interest.
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