Genetic homogeneity in South American tomato pinworm, Tuta absoluta: a new invasive pest to oriental region
- 75 Downloads
South American tomato leaf miner, Tuta absoluta (Meyrick, 1917) (Lepidoptera: Gelechiidae), is an important invasive pest of Tomato which invaded India and Nepal in 2014 and 2016, respectively. In the present study, samples from five localities of India and one from Nepal were used for the investigation of genetic diversity of T. absoluta by employing a fragment in the mtDNA gene-encoding cytochrome oxidase I (COI). Based on the partial COI gene, high genetic homogeneity was detected in T. absoluta populations of India and Nepal with rest of the world. Less nucleotide diversity (π 0.00137) was also detected in the populations of T. absoluta from different countries. This is first attempt to analyze molecular data for this new invasive species from India and Nepal.
KeywordsCytochrome oxidase Invasive pest India Nepal Tomato Tuta absoluta
We are grateful to Head, Division of Entomology, Indian Agricultural Research Institute, Pusa Campus, New Delhi for her guidance. We thank DST-SERB (SB/YS/LS-126/2014) for the financial assistance to conduct this work.
SPR collected and identified specimens. TS performed molecular work. Conceptualization: SPR and NMM. Data curation: SPR, TS, and SSS. Data analysis: SPR and ASRB. Supervision: SPR and KC. Funding acquisition: SPR. All authors read, reviewed and approved the manuscript.
Compliance with ethical standards
Conflict of interest
The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article.
- Bajracharya ASR, Mainali RP, Bhat B, Bista S, Shashank PR, Meshram NM (2016) The first record of South American tomato leaf miner, Tuta absoluta (Meyrick 1917) (Lepidoptera: Gelechiidae) in Nepal. J Entomol Zool Stud 4:1359–1363Google Scholar
- Campos MR, Biondi A, Adiga A, Guedes RN, Desneux N (2017) From the Western Palaearctic region to beyond: Tuta absoluta 10 years after invading Europe. J Pest Sci 1:1–10Google Scholar
- Desneux N, Wajnberg E, Wyckhuys KAG, Burgio G, Arpaia S, Narva´ez-Vasquez CA, Gonza´lez-Cabrera J, Catala´n Ruescas D, Tabone E, Frandon J, Pizzol J, Poncet C, Cabello T, Urbaneja A (2010) Biological invasion of European tomato crops by Tuta absoluta: ecology, history of invasion and prospects for biological control. J Pest Sci 83:197–215CrossRefGoogle Scholar
- Flores LV, Gilardon E, Gardenal CN (2003) Genetic structure of populations of Tuta absoluta Meyrick (Lepidoptera: Gelechiidae). J Basic Appl Genet 15:29–32Google Scholar
- Hayden JE, Lee S, Passoa SC, Young J, Landry JF, Nazari V, Mally R, Somma LA, Ahlmark KM (2013) Digital identification of microlepidoptera on solanaceae. USDA-APHIS-PPQ identification technology program (ITP). Fort Collins, CO. http://idtools.org/id/leps/micro/. Accessed 09 Feb 2017
- ICAR (2015) Tuta absoluta: A new invasive pest alert. Indian Council of Agricultural Research New Delhi, India, 2015. http://www.icar.org.in/en/node/8600. Accessed 23 Jun 2016
- IHD (2017) Indian Horticulture Database. National Horticulture Board, Ministry of Agriculture, New Delhi. http://nhb.gov.in/PDFViwer.aspx?enc=3ZOO8K5CzcdC/Yq6HcdIxC0U1kZZenFuNVXacDLxz28=. Accessed 18 Nov 17
- Ito K, Nishikawa H, Shimada T, Ogawa K, Minamiya Y, Tomoda M, Nakahira K, Kodama R, Fukuda T, Arakawa R (2011) Analysis of genetic variation and phylogeny of the predatory bug, Pilophorus typicus, in Japan using mitochondrial gene sequences. J Insect Sci 11:18CrossRefPubMedPubMedCentralGoogle Scholar
- Kumari DA, Anitha G, Anitha V, Lakshmi BK, Vennila S, Rao NH (2015) New record of leaf miner, Tuta absoluta (Meyrich) in Tomato. Insect Environ 20:136–138Google Scholar
- Larrain PS (1986) Plagas del tomate. IPA La Platina 39:30–35Google Scholar
- Margam VM, Coates BS, Ba MN, Sun W, Binso-Dabire CL, Baoua I, Ishiyaku MF, Shukle JT, Hellmich RL, Covas FG, Ramasamy S, Armstrong J, Pittendrigh BR, Murdock LL (2011) Geographic distribution of phylogenetically-distinct legume pod borer, Maruca vitrata (Lepidoptera: Pyraloidea: Crambidae). Mol Biol Rep 38:893–903CrossRefPubMedGoogle Scholar
- Reil JB, San Jose M, Rubinoff D (2016) Low variation in nuclear and mitochondrial DNA inhibits resolution of invasion pathways across the Pacific for the Coconut Rhinoceros Beetle (Scarabeidae: Oryctes rhinoceros). Proc Hawaiian Entomol Soc 48:57–69Google Scholar
- Shanmugam PS, Ramaraju K, Indhumathi K (2016) First record of South American tomato moth, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) in Tamil Nadu, India. Entomon 41:61–66Google Scholar
- Shashank PR, Suroshe SS, Singh PK, Chandrashekar K, Nebapure SM, Meshram NM (2016) Report of invasive tomato leaf miner, Tuta absoluta (Lepidoptera: Gelechiidae) from northern India. Indian J Agric Sci 86:1635–1636Google Scholar
- Valade R, Kenis M, Hernandez-lopez A, Augustin S, Mari Mena N, Magnoux E, Rougerie R, Lakatos F, Roques A, Lopez-Vaamonde C (2009) Mitochondrial and microsatellite DNA markers reveal a Balkan origin for the highly invasive horse-chestnut leaf miner Cameraria ohridella (Lepidoptera, Gracillariidae). Mol Ecol 18:3458–3470CrossRefPubMedGoogle Scholar
- Vargas H (1970) Observaciones sobre la biologia y enemigos naturales de la polilla del tomate, Gnorimoschema absoluta (Meyrick) (Lepidoptera: Gelechiidae). Idesia 1:75–110Google Scholar