3 Biotech

, 8:308 | Cite as

Selection and evaluation of microorganisms for biodegradation of agricultural plastic film

  • Jing Zhang
  • Jing Chen
  • Ruimin Jia
  • Zhihen Dun
  • Baotong Wang
  • Xiaopin Hu
  • Yang Wang
Original Article


Three Bacillus amyloliquefaciens isolates (HK1, GSDM02, and GSDM15) were tested for effectiveness in biodegradation of plastic films. Isolates were screened by plate on carbon-free medium and by using the clear-zone formation test. Their biodegradation ability was analyzed based on: film weight reduction, pH change of the fluid medium, a soil microbial biomass carbon test, scanning electron microscopy (SEM), and Fourier transform infrared spectrometry (FTIR). Polyvinyl alcohol (PVA) clear-zone and film weight reduction results revealed that the strain with a bigger clear-zone had a better biodegradation effect, that PVA can be evenly distributed in the medium, and that PVA can be a substitution for polyethylene in screening the biodegradation of strains. SEM and FTIR revealed that HK1 can tear the film apart and make surface chemical changes within 30 days. HK1 exhibited a better biodegradation effect in all tests, indicating its potential for helping solve the plastic pollution problems.


Biodegradation Agricultural plastic film Polyvinyl alcohol Bacillus amyloliquefaciens HK1 



This study was financially supported by the Demonstration and Promotion of Microbial Degradation Film Technology in Gansu Province Grant (K3380216177). We are grateful to Professor Xiaoping Hu and Professor Fusako Kawai for providing us with more than 190 strains. We thank Professor John Richard Schrock for proofreading the text. Thanks goes to the Department of Plant Protection, Northwest Agriculture and Forest University for providing research facilities and thanks The Management Station for the Quality Construction of Cultivated Land in Gansu Province for assistance in field test.

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interests.

Supplementary material

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of Plant ProtectionNorthwest A&F UniversityXianyangChina
  2. 2.The Management Station for the Quality Construction of Cultivated Land in Gansu ProvinceLanzhouChina

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