Advertisement

3 Biotech

, 8:308 | Cite as

Selection and evaluation of microorganisms for biodegradation of agricultural plastic film

  • Jing Zhang
  • Jing Chen
  • Ruimin Jia
  • Zhihen Dun
  • Baotong Wang
  • Xiaopin Hu
  • Yang Wang
Original Article

Abstract

Three Bacillus amyloliquefaciens isolates (HK1, GSDM02, and GSDM15) were tested for effectiveness in biodegradation of plastic films. Isolates were screened by plate on carbon-free medium and by using the clear-zone formation test. Their biodegradation ability was analyzed based on: film weight reduction, pH change of the fluid medium, a soil microbial biomass carbon test, scanning electron microscopy (SEM), and Fourier transform infrared spectrometry (FTIR). Polyvinyl alcohol (PVA) clear-zone and film weight reduction results revealed that the strain with a bigger clear-zone had a better biodegradation effect, that PVA can be evenly distributed in the medium, and that PVA can be a substitution for polyethylene in screening the biodegradation of strains. SEM and FTIR revealed that HK1 can tear the film apart and make surface chemical changes within 30 days. HK1 exhibited a better biodegradation effect in all tests, indicating its potential for helping solve the plastic pollution problems.

Keywords

Biodegradation Agricultural plastic film Polyvinyl alcohol Bacillus amyloliquefaciens HK1 

Notes

Acknowledgements

This study was financially supported by the Demonstration and Promotion of Microbial Degradation Film Technology in Gansu Province Grant (K3380216177). We are grateful to Professor Xiaoping Hu and Professor Fusako Kawai for providing us with more than 190 strains. We thank Professor John Richard Schrock for proofreading the text. Thanks goes to the Department of Plant Protection, Northwest Agriculture and Forest University for providing research facilities and thanks The Management Station for the Quality Construction of Cultivated Land in Gansu Province for assistance in field test.

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interests.

Supplementary material

13205_2018_1329_MOESM1_ESM.jpg (816 kb)
Supplementary material 1 (JPG 815 KB)
13205_2018_1329_MOESM2_ESM.jpg (347 kb)
Supplementary material 2 (JPG 347 KB)
13205_2018_1329_MOESM3_ESM.jpg (28.6 mb)
Supplementary material 3 (JPG 29265 KB)
13205_2018_1329_MOESM4_ESM.jpg (1.3 mb)
Supplementary material 4 (JPG 1307 KB)

References

  1. Abou-Zeid DM, Müller RJ, Deckwer WD (2001) Anaerobic biodegradation of natural and synthetic polyesters. Dissertation, Technical University Braunschweig, GermanyGoogle Scholar
  2. Albertsson AC, Karlsson S (1990) The influence of biotic and abiotic environments on the degradation of polyethylene. Prog Polym Sci 15:177–192CrossRefGoogle Scholar
  3. Albertsson AC, Barenstedt C, Karlsson S (2010) Abiotic degradation products from enhanced environmentally degradable polyethylene. Acta Polym 45:97–103CrossRefGoogle Scholar
  4. Balasubramanian V, Natarajan K, Hemambika B, Ramesh N, Sumathi CS, Kottaimuthu R, Rajesh Kannan V (2010) High-density polyethylene (HDPE)-degrading potential bacteria from marine ecosystem of Gulf of Mannar, India. Lett Appl Microbiol 51:205–211Google Scholar
  5. Chatterjee S, Roy B, Roy D, Bajat R (2010) Enzyme-mediated biodegradation of heat treated commercial polyethylene by Staphylococcal species. Polymer Degradation Stability 95:195–200CrossRefGoogle Scholar
  6. Dey U, Mondal NK, Das K, Dutta S (2012) An approach to polymer degradation through microbes. IOSR J Pharm 2:385–388Google Scholar
  7. Emadian SM, Onay TT, Demirel B (2016) Biodegradation of bioplastics in natural environments. Waste Manag 59:526–536CrossRefGoogle Scholar
  8. He ZL (1997) Soil microbial biomass and its significance in nutrient cycling and environmental quality assessment. Soil 2:61–69Google Scholar
  9. Hotta Y, Hiraoka R, Yamaoka T (1997) Effect of particle size and polarity of long-chain molecules in polymeric films on the supercooling temperature. High Perform Polym 9:369–383CrossRefGoogle Scholar
  10. Jun Y, Yu Y, Wei-Min W, Jiao Z, Lei J (2014) Evidence of polyethylene biodegradation by bacterial strains from the guts of plastic-eating waxworms. Environ Sci Technol 48:13776–13784CrossRefGoogle Scholar
  11. Kawai F, Watanabe M, Shibata M, Shigeo Y, Yasuhiro S, Shizue H (2004) Comparative study on biodegradability of polyethylene wax by bacteria and fungi. Polym Degrad Stab 86:105–114CrossRefGoogle Scholar
  12. Kirk JL, Beaudette LA, Hart M, Moutoglisc P (2004) Methods of studying soil microbial diversity. J Microbiol Methods 58:169–188CrossRefGoogle Scholar
  13. Llop C, Pérez A (2011) Technology available for recycling agricultural mulch film. Makromolekulare Chemie Macromolecular Symposia 57:115–121CrossRefGoogle Scholar
  14. Mor R, Sivan A (2008) Biofilm formation and partial biodegradation of polystyrene by the actinomycete Rhodococcus ruber. Biodegradation 19:851–858CrossRefGoogle Scholar
  15. Nishida H, Tokiwa Y (1993) Distribution of poly (β-hydroxybutyrate) and poly (ε-caprolactone) aerobic degrading microorganisms in different environments. J Environ Polym Degrad 1:227–233CrossRefGoogle Scholar
  16. Nowak B, Pająk J, Drozd-Bratkowicz M, Rymarz M (2011) Microorganisms participating in the biodegradation of modified polyethylene films in different soils under laboratory conditions. Int Biodeterior Biodegrad 65:757–767CrossRefGoogle Scholar
  17. Peixoto B, Silva LP, Krüger RH (2017) Brazilian cerrado soil reveals an untapped microbial potential for unpretreated polyethylene biodegradation. J Hazard Mater 324:634–644CrossRefGoogle Scholar
  18. Pometto AL, Lee BT, Johnson KE (1992) Production of an extracellular polyethylene-degrading enzyme(s) by Streptomyces species. Appl Environ Microbiol 58:731–733Google Scholar
  19. Quecholac-Piña X, García-Rivera MA, Espinosa-Valdemar RM, Vázquez-Morillas A, Beltrán-Villavicencio M, Cisneros-Ramos AL (2016) Biodegradation of compostable and oxodegradable plastic films by backyard composting and bioaugmentation. Environ Sci Pollut Res 24:1–6Google Scholar
  20. Rajandas H, Parimannan S, Sathasivam K, Ravichandran M, Yin LS (2012) A novel FTIR-ATR spectroscopy based technique for the estimation of low-density polyethylene biodegradation. Polym Test 31:1094–1099CrossRefGoogle Scholar
  21. Restrepo-Flórez J, Bassi A, Thompson MR (2014) Microbial degradation and deterioration of polyethylene—a review. Int Biodeterior Biodegrad 88:83–90CrossRefGoogle Scholar
  22. Santo M, Weitsman R, Sivan A (2013) The role of the copper-binding enzyme—laccase—in the biodegradation of polyethylene by the actinomycete Rhodococcus ruber. Int Biodeterior Biodegrad 84:204–210CrossRefGoogle Scholar
  23. Shah AA, Hasan F, Hameed A, Ahmed S (2008) Biological degradation of plastics: a comprehensive review. Biotechnol Adv 26:246–265CrossRefGoogle Scholar
  24. Sivan A (2011) New perspectives in plastic biodegradation. Curr Opin Biotechnol 22:422–426CrossRefGoogle Scholar
  25. Tribedi P, Sil AK (2013) Low-density polyethylene degradation by Pseudomonas sp. AKS2 biofilm. Environ Sci Pollut Res 20:4146–4153CrossRefGoogle Scholar
  26. Usami A, Nakaya S, Nakahashi H, Miyazawa M (2014) Chemical composition and aroma evaluation of volatile oils from edible mushrooms(Pleurotus salmoneostramineus and Pleurotus sajor-caju). J Oleo Sci 63:1323–1332CrossRefGoogle Scholar
  27. Xie YP, Xue JL (2005) Ultra-morphology and chemical composition of waxes secreted by two wax scale insects, Ceroplastes ceriferus (Fabricius) and C. Japonicus Green (Homoptera: Coccidae). Acta Entomol Sinica 48: 837–848Google Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of Plant ProtectionNorthwest A&F UniversityXianyangChina
  2. 2.The Management Station for the Quality Construction of Cultivated Land in Gansu ProvinceLanzhouChina

Personalised recommendations