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3 Biotech

, 8:291 | Cite as

Complete genome sequence of Bacillus sp. HBCD-sjtu, an efficient HBCD-degrading bacterium

  • Syed Bilal Shah
  • Fawad Ali
  • Ling Huang
  • Weiwei Wang
  • Ping Xu
  • Hongzhi TangEmail author
Genome Reports

Abstract

Environmental pollution caused by the release of industrial chemicals is currently one of the most important environmental harms. Manufacturing chemicals can be biodegraded, and valuable intermediates can be used as pharmacophores in drug targeting and have several other useful purposes. Hexabromocyclododecane (HBCD), a non-aromatic brominated flame retardant, is a toxic compound that consists of a cycloaliphatic ring of 12 carbon atoms to which six bromine atoms are attached. It is formed by bromination of cis–trans–trans-1,5,9-cyclododecatriene, but its use is now restricted in several countries, because it is an environmental pollutant. Little is known about whether bacteria can degrade HBCD. A bacterial strain that degrades HBCD was recently isolated using enrichment culture techniques. Based on morphological, biochemical and phylogenetic analysis this isolate was categorized as Bacillus cereus and named strain HBCD-sjtu. Maximum growth and HBCD-degrading activity were observed when this strain was grown at 30 °C, pH 7.0 and 200 RPM in mineral salt medium containing 0.5 mm HBCD. The genome of strain HBCD-sjtu, which consists of only one circular chromosome, was sequenced. This whole genome sequence will be crucial for illuminating the molecular mechanisms of HBCD degradation.

Keywords

HBCD Brominated flame retardant BLAST Degradation Whole genome sequence Bacillus cereus 

Notes

Acknowledgements

This work was supported by grants from the Chinese National Science Foundation for Excellent Young Scholars (31422004), by the grants from the Chinese National Science Foundation (31770114), and by the ‘Shuguang Program’ (17SG09) supported by Shanghai Education Development Foundation and Shanghai Municipal Education Commission.

Compliance with ethical standards

Conflict of interest

The authors declared no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Syed Bilal Shah
    • 1
  • Fawad Ali
    • 1
  • Ling Huang
    • 1
  • Weiwei Wang
    • 1
  • Ping Xu
    • 1
  • Hongzhi Tang
    • 1
    Email author
  1. 1.State Key Laboratory of Microbial Metabolism, and School of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghaiPeople’s Republic of China

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