Assessment of chemical and genetic variability in Tanacetum gracile accessions collected from cold desert of Western Himalaya
Genetic diversity is essential for survival and adaptation of high altitude plants such as those of Tanacetum genus, which are constantly exposed to environmental stress. We collected flowering shoots of ten accessions of Tanacetum gracile Hook.f. & Thomson (Asteraceae) (Tg 1–Tg 10), from different regions of cold desert of Western Himalaya. Chemical profile of the constituents, as inferred from GC–MS, exhibited considerable variability. Percentage yield of essential oil ranged from 0.2 to 0.75% (dry-weight basis) amongst different accessions. Tg 1 and Tg 6 were found to produce high yields of camphor (46%) and lavandulol (41%), respectively. Alpha-phellendrene, alpha-bisabool, p-cymene and chamazulene were the main oil components in other accessions. Genetic variability among the accessions was studied using RAPD markers as well as by sequencing and analyzing nuclear 18S rDNA, and plastid rbcL and matK loci. The polymorphic information content (PIC) of RAPD markers ranged from 0.18 to 0.5 and the analysis clustered the accessions into two major clades. The present study emphasized the importance of survey, collection, and conservation of naturally existing chemotypes of medicinal and aromatic plants, considering their potential use in aroma and pharmaceutical industry.
KeywordsEssential oil GC–MS ITS MatK RAPD RbcL
VM and RC were supported by CSIR-Senior/Junior research fellowships, respectively. SGG acknowledges the financial support for this work from CSIR 12th FYP projects ‘BioprosPR’ (BSC0106) of Council of Scientific and Industrial Research (CSIR).
VM carried out genetic marker studies, wrote the manuscript and prepared figures. RC helped VM in preparation of manuscript and figures. SK and SK collected T. gracile accessions and carried out extraction of essential oil. BS performed taxonomical identification and authentication of plant. KB performed GC-MS of the essential oils. SGG designed the study and edited the manuscript and figures. YSB and SK provided critical inputs for the study as well as during preparation of manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they do not have any conflict of interest.
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