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3 Biotech

, 8:288 | Cite as

Extractive purification of recombinant thermostable lipase from fermentation broth of Escherichia coli using an aqueous polyethylene glycol impregnated resin system

  • Joo Shun Tan
  • Sahar Abbasiliasi
  • Arbakariya B. Ariff
  • Hui Suan Ng
  • Mohamad Hafizi Abu Bakar
  • Yin Hui Chow
Original Article
  • 66 Downloads

Abstract

This study aimed at recovery of thermostable lipase from Escherichia coli BL21 using porous glass beads grafted with polyethylene glycol (PEG) in aqueous impregnated resins system (AIRS). The influencing parameters such as concentration and pH of extraction solution, concentration of NaCl, size of the beads, and pH of the desorption solution on the partition behaviour of lipase were evaluated. Smaller adsorbent (4 mm) had a 65.5% of recovery yield with approximately two-fold higher purification factor compared to that obtained with the larger adsorbent. Recombinant lipase was purified successfully using AIRS with a purification factor of 7.6 and yield of 78.4% under optimum conditions of 18% (w/w) PEG 4000, 10% (w/w) of potassium citrate at pH 9 with 3% (w/w) of NaCl. Optimum desorption was obtained with 4.0 mm of porous glass beads at pH 9.

Keywords

Aqueous impregnated resins system Aqueous phase Purification Recovery Lipase 

Notes

Acknowledgements

This work was funded by Universiti Sains Malaysia under USM Short Term Grant (PO4865, 304/PTENKIND/6315138).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Joo Shun Tan
    • 1
  • Sahar Abbasiliasi
    • 2
    • 3
  • Arbakariya B. Ariff
    • 4
  • Hui Suan Ng
    • 5
  • Mohamad Hafizi Abu Bakar
    • 1
  • Yin Hui Chow
    • 6
  1. 1.Bioprocess Technology, School of Industrial TechnologyUniversiti Sains MalaysiaGelugorMalaysia
  2. 2.Department of Microbiology, Faculty of Biotechnology and Biomolecular SciencesUniversiti Putra MalaysiaSerdangMalaysia
  3. 3.Halal Products Research InstituteUniversiti Putra MalaysiaSerdangMalaysia
  4. 4.Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular SciencesUniversiti Putra MalaysiaSerdangMalaysia
  5. 5.Department of Food Science and Nutrition, Faculty of Applied SciencesUCSI UniversityKuala LumpurMalaysia
  6. 6.School of EngineeringTaylor’s UniversitySubang JayaMalaysia

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