3 Biotech

, 8:271 | Cite as

Bacterial cellulase treatment for enhancing reactivity of pre-hydrolysed kraft dissolving pulp for viscose

  • Chakarvati Sango
  • Prabhjot Kaur
  • Nishi K. Bhardwaj
  • Jitender SharmaEmail author
Original Article


To improve the process economy of reactivity improvement, crude cellulase from Bacillus subtilis was employed for the treatment and significant dissolving pulp properties were analyzed. With increase in enzyme dose from 0.25 to 2 U/g o.d. pulp, improvement in Fock reactivity and alkali solubilities (S10 and S18) were observed with simultaneous reduction in viscosity and yield. Fourier transform infrared spectroscopy and scanning electron microscopy were used to observe the molecular level effects on dissolving grade pulp. The most suitable cellulase dose for reactivity improvement with lowering of viscosity was 0.25 U/g o.d. pulp. With increases in enzyme dose, alkali solubilities (S10 and S18) of dissolving pulp showed continuous increment, while alpha-cellulose of pulp showed reduction due to chain scission of long cellulose fiber fraction.


Dissolving pulp Crude cellulase Reactivity Viscose Alkali solubility Bacillus subtilis 



The authors are thankful to the Director of the Avantha Centre for Industrial Research & Development, Yamuna Nagar, Haryana, India, for providing the facilities to complete this work.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Chakarvati Sango
    • 1
  • Prabhjot Kaur
    • 2
  • Nishi K. Bhardwaj
    • 2
  • Jitender Sharma
    • 1
    Email author
  1. 1.Department of BiotechnologyKurukshetra UniversityKurukshetraIndia
  2. 2.Avantha Centre for Industrial Research and DevelopmentYamuna NagarIndia

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