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3 Biotech

, 8:265 | Cite as

Increasing of activity and thermostability of cold active butanol-tolerant endoglucanase from a marine Rhodococcus sp. under high concentrations of butanol condition

  • Xuhao Zeng
  • Dongsheng Xue
Original Article
  • 85 Downloads

Abstract

Optimum pH and temperature of the endoglucanase from Rhodococcus sp. was 5.0 and 40 °C. The endoglcuanase activity was 1.18 folds higher in 5% butanol solution than that in butanol-free solution. Melt point temperature was 2.5 °C higher in 5% butanol solution (50.0 °C) than that in butanol-free solution (47.5 °C). At 45–60 °C, Gbbs-free energy change, ∆G, was higher in 5% butanol solution than that in butanol-free solution. The content of α-helix and β-sheet increased in 5% butanol solution. The increasing of α-helix and β-sheet content led to higher activity and better thermostability in butanol solution. The cold active butanol-tolerant endoglucanase was valuable for biobutanol production by a simultaneous saccharification and fermentation process.

Keywords

Endoglucanase Butanol tolerant Cold active Rhodococcus sp 

Notes

Acknowledgements

This work was supported by national natural science foundation of China (31271928) and Hubei Provincial Department of Education (Project: Design of a butanol-tolerant endoglucanase and butanol-tolerant mechanism, 2017B041).

Compliance with ethical standards

Conflict of interest

There are no conflict of interest exists for all participating authors. And the article also complies with the ethical standard.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Key Laboratory of Industrial Microbiology, Hubei Provincial Cooperative Innovation Center of Industrial FermentationHubei University of TechnologyWuhanPeople’s Republic of China

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