Increasing of activity and thermostability of cold active butanol-tolerant endoglucanase from a marine Rhodococcus sp. under high concentrations of butanol condition
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Optimum pH and temperature of the endoglucanase from Rhodococcus sp. was 5.0 and 40 °C. The endoglcuanase activity was 1.18 folds higher in 5% butanol solution than that in butanol-free solution. Melt point temperature was 2.5 °C higher in 5% butanol solution (50.0 °C) than that in butanol-free solution (47.5 °C). At 45–60 °C, Gbbs-free energy change, ∆G, was higher in 5% butanol solution than that in butanol-free solution. The content of α-helix and β-sheet increased in 5% butanol solution. The increasing of α-helix and β-sheet content led to higher activity and better thermostability in butanol solution. The cold active butanol-tolerant endoglucanase was valuable for biobutanol production by a simultaneous saccharification and fermentation process.
KeywordsEndoglucanase Butanol tolerant Cold active Rhodococcus sp
This work was supported by national natural science foundation of China (31271928) and Hubei Provincial Department of Education (Project: Design of a butanol-tolerant endoglucanase and butanol-tolerant mechanism, 2017B041).
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Conflict of interest
There are no conflict of interest exists for all participating authors. And the article also complies with the ethical standard.
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