3 Biotech

, 8:208 | Cite as

Enhanced saccharification of rice straw using combined ultra-high pressure and ionic liquid microemulsion pretreatments

  • Jing Gao
  • Caiju Zheng
  • Tingru Tan
  • Shucheng Liu
  • Hongwu Ji
Original Article


Energy efficiency ratio is significant in completely estimating lignocellulosic biomass pretreatment. In this work, rice straw (RS) was pretreated by ultra-high pressure (UHP), ionic liquid microemulsion (ILM), and a combination of UHP and ILM (ILM + UHP) at mild temperature. The chemical composition, crystalline structure, surface morphology, and enzymatic hydrolysis of untreated and pretreated RS samples were compared. After ILM pretreatment ([Emim]Ac/cyclohexane/Triton X-100/n-butanol = 0.25/0.15/0.45/0.15) at 500 MPa, 50 °C for 4 h, the cellulose content of the regenerated RS increased by 62.5, 66.2% of the lignin was removed, 37.3% of crystallinity index decreased, and the reducing sugar yield of 89.6% was achieved. All results show that the ILM + UHP pretreatments were more effective than sole UHP or ILM treatment at low temperature.


Lignocellulosic biomass Combination pretreatment Ultra-high pressure Ionic liquid microemulsion Enzymatic hydrolysis 



This work was supported by Fund for National Natural Science Foundation of China (21706040), Guangdong Province Innovation School Project (2015KQNCX061, GDOU2015050240), Fund of Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, China (B16387), and Fund of Scientific Research Start-up Funds of Guangdong Ocean University (E15178).

Compliance with ethical standards

Conflict of interests

The authors declare no competing financial interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Jing Gao
    • 1
  • Caiju Zheng
    • 1
  • Tingru Tan
    • 1
  • Shucheng Liu
    • 1
  • Hongwu Ji
    • 1
  1. 1.Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, College of Food Science and TechnologyGuangdong Ocean UniversityZhanjiangChina

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