First global transcriptome analysis of brown algae Macrocystis integrifolia (Phaeophyceae) under marine intertidal conditions
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To understand the physiological responses of the brown macroalga Macrocystis integrifolia during the marine tidal cycle, two RNA libraries were prepared from algal frond samples collected in the intertidal zone (0 m depth) and subtidal zone (10 m depth). Samples collected from intertidal zone during low tide was considered as abiotic stressed (MI0), while samples collected from subtidal zone was considered as control (MI10). Both RNA libraries were sequenced on Illumina NextSeq 500 which generated approx. 46.9 million and 47.7 million raw paired-end reads for MI0 and MI10, respectively. Among the representative transcripts (RTs), a total of 16,398 RTs (39.20%) from MI0 and 21,646 RTs (39.24%) from MI10 were successfully annotated. A total of 535 unigenes (271 upregulated and 264 downregulated) showed significantly altered expression between MI0 and MI10. In abiotic-stressed condition (MI0), the relative expression levels of genes associated with antioxidant defenses (vanadium-dependent bromoperoxidase, glutathione S-transferase, lipoxygenase, serine/threonine-protein kinase, aspartate Aminotransferase, HSPs), water transport (aquaporin), photosynthesis (light-harvesting complex) protein were significantly upregulated, while in control condition (MI10) most of the genes predominantly involved in energy metabolism (NADH-ubiquinone oxidoreductase/NADH dehydrogenase, NAD(P)H-Nitrate reductase, long-chain acyl-CoA synthetase, udp-n-acetylglucosamine pyrophosphorylase) were overexpressed.
KeywordsMacrocystis integrifolia (Phaeophyceae) Abiotic stress Transcriptome analysis De novo assembly Differential gene expression
This work was supported by the Grant no 352-PNICP-PIBA-2014 from Programa Nacional de Innovación para la Competitividad y Productividad (INNOVATE PERU), Ministry of Production of Peru. Erika Salavarría was funded by scholarship program “Academia 2010”. SENESCYT. Ecuador. We acknowledge Peruvian Seaweeds S.R.L. and Gunter Villena for logistic facilitated for the sampling. We acknowledge Genotypic Technology Pvt. Ltd Bengaluru, India for additional analysis. We want to thank Dr. Marcel Gutiérrez-Correa (RIP) for his extraordinary support and useful advice during the development of this work.
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Conflict of interest
The authors declare that they don’t have any conflict of interest.
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