Antimicrobial and anticancer activity of AgNPs coated with Alphonsea sclerocarpa extract
- 17 Downloads
The synthesis and characterization of an aggregate of AgNPs coated with plant extract (PE) from Alphonsea sclerocarpa and its significant antimicrobial activity and inhibition on K562 (blood cancer) cells have been appended in the article. Synthesis of aggregate [(AgNPs)-(PE)] has been followed by a facile eco-friendly approach without using any harmful chemicals. The morphology of an aggregate [(AgNPs)-(PE)] was confirmed by TEM and SEM microscopic characterizations. Properties like solid state, the presence of functional groups, and elemental composition have been characterized through the XRD, FTIR, and EDAX. The biocompatibility of synthesized aggregate of [(AgNPs)-(PE)] was confirmed by the MTT assay. An in vitro cell (HEK293)-based studies were performed for the biocompatibility tests and it is found that the aggregate [(AgNPs)-(PE)] is not harmful to normal/healthy cells. Even though A. sclerocarpa show the antimicrobial (antibacterial and antifungal) activity, it has been further enhanced with the developed aggregate of [(AgNPs)-(PE)]. Furthermore, it has been extended to examine the cellular inhibition on K562 cells and obtained > 75% cell inhibition for 24 h treated cells.
Keywords[(AgNPs)-PE)] Silver nanoparticles Antimicrobial activity Cell inhibition
The authors would like to acknowledge DST-FIST, Department of Physics, Acharya Nagarjuna University for providing IR facility, University of Hyderabad, for providing SEM with EDAX and XRD facility.
Compliance with ethical standards
Conflict of interest
Both the first and second authors have equal contribution to the paper.
- El-Chaghaby GA, Ahmad AF (2011) Biosynthesis of silver nanoparticles using Pistacia lentiscus leaves extract and investigation of their antimicrobial effect. Orient J Chem 27(3):929–936Google Scholar
- Kumar VA, Uchida T, Mizuki T, Nakajima Y, Katsube Y, Hanajiri T, Maekawa T (2016) Synthesis of nanoparticles composed of silver and silver chloride for a plasmonic photocatalyst using an extract from a weed Solidago altissima (goldenrod). Adv Nat Sci 7(1):015002Google Scholar
- Nagababu P, Uma Maheswara Rao V (2017) Pharmacological assessment, green synthesis and characterization of silver nanoparticles of sonneratia apetala Buch.-Ham. leaves. J App Pharm Sci 7(8):175–182Google Scholar
- Prasad DN (2009) Antioxidant activity of Alphonsea sclerocarpa bark. Res J Pharmacol Pharmacodynamics 1(2):66–69Google Scholar
- Song HY, Ko KK, Oh IH, Lee BT (2006) Fabrication of silver nanoparticles and their antimicrobial mechanisms. Eur Cell Mater 11(1):58Google Scholar
- Suman Joshi DSD, Venkata Rao G, Satya Prasad M, Kishore Babu M, Surya Narayana S, Krishna Satya A (2017) Phytochemical screening and evaluation of antioxidant, antibacterial and antifungal activity of medicinal plant Alphonsea sclerocarpa Thaw. J Pharmacogn Phytochem 6(4):1280–1286Google Scholar
- Veerasamy R, Xin TZ, Gunasagaran S, Xiang TFW, Yang EFC, Jeyakumar N, Dhanaraj SA (2011) Biosynthesis of silver nanoparticles using mangosteen leaf extract and evaluation of their antimicrobial activities. J Saudi Chem Soc 15:113–120. https://doi.org/10.1016/j.jscs.2010.06.004 CrossRefGoogle Scholar