3 Biotech

, 8:69 | Cite as

Bioethanol production from microwave-assisted acid or alkali-pretreated agricultural residues of cassava using separate hydrolysis and fermentation (SHF)

  • N. S. Pooja
  • M. S. Sajeev
  • M. L. Jeeva
  • G. PadmajaEmail author
Original Article


The effect of microwave (MW)-assisted acid or alkali pretreatment (300 W, 7 min) followed by saccharification with a triple enzyme cocktail (Cellic, Optimash BG and Stargen) with or without detoxification mix on ethanol production from three cassava residues (stems, leaves and peels) by Saccharomyces cerevisiae was investigated. Significantly higher fermentable sugar yields (54.58, 47.39 and 64.06 g/L from stems, leaves and peels, respectively) were obtained after 120 h saccharification from MW-assisted alkali-pretreated systems supplemented (D+) with detoxification chemicals (Tween 20 + polyethylene glycol 4000 + sodium borohydride) compared to the non-supplemented (D0) or MW-assisted acid-pretreated systems. The percentage utilization of reducing sugars during fermentation (48 h) was also the highest (91.02, 87.16 and 89.71%, respectively, for stems, leaves and peels) for the MW-assisted alkali-pretreated (D+) systems. HPLC sugar profile indicated that glucose was the predominant monosaccharide in the hydrolysates from this system. Highest ethanol yields (YE, g/g), fermentation efficiency (%) and volumetric ethanol productivity (g/L/h) of 0.401, 78.49 and 0.449 (stems), 0.397, 77.71 and 0.341 (leaves) and 0.433, 84.65 and 0.518 (peels) were also obtained for this system. The highest ethanol yields (ml/kg dry biomass) of ca. 263, 200 and 303, respectively, for stems, leaves and peels from the MW-assisted alkali pretreatment (D+) indicated that this was the most effective pretreatment for cassava residues.


Cassava residues Microwave Acid/alkali pretreatment SHF Bioethanol 



The first author gratefully acknowledges the research fellowship granted for the study by the Kerala State Council for Science, Technology and Environment (KSCSTE), Govt. of Kerala. Authors are thankful to the Director, ICAR-CTCRI for the facilities provided for the study and Dr. J. Sreekumar, Principal Scientist (Agricultural Statistics), ICAR-CTCRI for the help extended in statistical analyses. The support extended for the HPLC analyses by Dr. A. N. Jyothi, Principal Scientist and Mr. V. R. Vishnu, Senior Research Fellow, ICAR-CTCRI is also thankfully acknowledged.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Division of Crop UtilizationICAR-Central Tuber Crops Research InstituteThiruvananthapuramIndia
  2. 2.Division of Crop ProtectionICAR-Central Tuber Crops Research InstituteThiruvananthapuramIndia

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