3 Biotech

, 8:67 | Cite as

Unveiling the role of ATP in amplification of intrinsic peroxidase-like activity of gold nanoparticles

  • Juhi Shah
  • Sanjay Singh
Original Article


Peroxidase enzyme-like activity of gold nanoparticles (AuNPs) is currently being investigated for the potential application in the several realms of biomedicines. However, little is explored about the peroxidase activity of AuNPs decorated with different surface charges. It is well-documented that the catalytic activity and the interaction with mammalian cells are significantly different among AuNPs carrying different surface charges. We have recently reported that ATP enhances the peroxidase-like activity of AuNPs and iron oxide nanoparticles. However, a comprehensive and systematic study to reveal the role of surface charge on nanoparticles peroxidase-like activity has not been studied. In this work, we have shown that AuNPs coated with PEG (PEG AuNPs), citrate (citrate AuNPs) or CTAB (CTAB AuNPs) exhibit varying peroxidase-like activity and the boosting effect imparted by ATP was also different. We found that the peroxidase-like activity of PEG AuNPs and citrate AuNPs is dependent on hydroxyl radical formation, whereas CTAB AuNPs did not show any significant activity under the same experimental conditions. We also studied the boosting effect of ATP on the peroxidase-like activity of PEG and citrate AuNPs. Although the use of ATP resulted in enhanced peroxidase-like activity; however, contrary to the expectation, it did not facilitate the enhanced production of hydroxyl radical. In further studies, we found that the likely mechanism of boosting effect by ATP is the stabilization of oxidized TMB after peroxidase reaction. ATP imparts stabilization to the oxidized TMB produced due to PEG AuNPs, citrate AuNPs as well as HRP.


Nanozymes Biomimetic nanoparticles Artificial enzymes Metal nanoparticles Hydroxyl radicals 



Juhi Shah would like to thank the Department of Science and Technology (DST), New Delhi for providing INSPIRE Junior Research Fellowship (JRF). The financial assistance for the Centre for Nanotechnology Research and Applications (CENTRA) by The Gujarat Institute for Chemical Technology (GICT) is acknowledged. The funding from the Department of Science and Technology-Science and Engineering Research Board (SERB) (Grant No.: ILS/SERB/2015-16/01) to Dr Sanjay Singh under the scheme of Start-Up Research Grant (Young Scientists) in Life Sciences is also gratefully acknowledged.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

13205_2017_1082_MOESM1_ESM.docx (446 kb)
Supplementary material 1 (DOCX 446 kb)


  1. Ahmed SR, Takemeura K, Li TC, Kitamoto N, Tanaka T, Suzuki T, Park EY (2017) Size-controlled preparation of peroxidase-like graphene-gold nanoparticle hybrids for the visible detection of norovirus-like particles. Biosens Bioelectron 87:558–565. CrossRefGoogle Scholar
  2. Andre R, Natálio F, Humanes M, Leppin J, Heinze K, Wever R, Schro¨der HC, Müller WE, Tremel W (2011) V2O5 nanowires with an intrinsic peroxidase-like activity. Adv Funct Mater 21:501–509CrossRefGoogle Scholar
  3. Asati A, Santra S, Kaittanis C, Nath S, Perez JM (2009) Oxidase-like activity of polymer-coated cerium oxide nanoparticles. Angew Chem Int Ed Engl 48:2308–2312CrossRefGoogle Scholar
  4. Asati A, Kaittanis C, Santra S, Perez JM (2011) pH-tunable oxidase-like activity of cerium oxide nanoparticles achieving sensitive fluorigenic detection of cancer biomarkers at neutral pH. Anal Chem 83(7):2547–2553. CrossRefGoogle Scholar
  5. Campbell AS, Dong C, Meng F, Hardinger J, Perhinschi G, Wu N, Dinu CZ (2014) Enzyme catalytic efficiency: a function of bio-nano interface reactions. ACS Appl Mater Interfaces 6(8):5393–5403. CrossRefGoogle Scholar
  6. Comotti M, Della Pina C, Matarrese R, Rossi M (2004) The catalytic activity of “naked” gold particles. Angew Chem Int Ed Engl 43(43):5812–5815. CrossRefGoogle Scholar
  7. Cui R, Han Z, Zhu JJ (2011) Helical carbon nanotubes: intrinsic peroxidase catalytic activity and its application for biocatalysis and biosensing. Chemistry 17(34):9377–9384. CrossRefGoogle Scholar
  8. Dalui A, Pradan B, Thupakula U, Khan AH, Kumar GS, Ghosh T, Satpati B, Acharya S (2015) Insight into the mechanism revealing the peroxidase mimetic catalytic activity of quaternary CuZnFeS nanocrystals: colorimetric biosensing of hydrogen peroxide and glucose. Nanoscale 7:9062–9074. CrossRefGoogle Scholar
  9. Deng HH, Hong GL, Lin FL, Liu AL, Xia XH, Chen W (2016) Colorimetric detection of urea, urease, and urease inhibitor based on the peroxidase-like activity of gold nanoparticles. Anal Chim Acta 915:74–80. CrossRefGoogle Scholar
  10. Fu S, Wang S, Zhang X, Qi A, Liu Z, Yu X, Chen C, Li L (2017) Structural effect of Fe3O4 nanoparticles on peroxidase-like activity for cancer therapy. Colloids Surf B Biointerfaces 154:239–245. CrossRefGoogle Scholar
  11. Gao L, Zhuang J, Nie L, Zhang J, Zhang Y, Gu N, Wang T, Feng J, Yang D, Perrett S, Yan X (2007) Intrinsic peroxidase-like activity of ferromagnetic nanoparticles. Nat Nanotechnol 2:577–583. CrossRefGoogle Scholar
  12. Heckert EG, Karakoti AS, Seal S, Self WT (2008) The role of cerium redox state in the SOD mimetic activity of nanoceria. Biomaterials 29(18):2705–2709. CrossRefGoogle Scholar
  13. Hong L, Liu AL, Li GW, Chen W, Lin XH (2013) Chemiluminescent cholesterol sensor based on peroxidase-like activity of cupric oxide nanoparticles. Biosens Bioelectron 43:1–5. CrossRefGoogle Scholar
  14. Jv Y, Li B, Cao R (2010) Positively-charged gold nanoparticles as peroxidase mimic and their application in hydrogen peroxide and glucose detection. Chem Commun 46(42):8017–8019. CrossRefGoogle Scholar
  15. Kong DM, Xu J, Shen HX (2010) Positive effects of ATP on G-quadruplex-hemin DNAzyme-mediated reactions. Anal Chem 82(14):6148–6153. CrossRefGoogle Scholar
  16. Lien CW, Chen YC, Chang HT, Huang CC (2013) Logical regulation of the enzyme-like activity of gold nanoparticles by using heavy metal ions. Nanoscale 5(17):8227–8234. CrossRefGoogle Scholar
  17. Lin Y, Zhao A, Tao Y, Ren J, Qu X (2013) Ionic liquid as an efficient modulator on artificial enzyme system: toward the realization of high-temperature catalytic reactions. J Am Chem Soc 135(11):4207–4210. CrossRefGoogle Scholar
  18. Lin Y, Huang Y, Ren J, Qu X (2014a) Incorporating ATP into biomimetic catalysts for realizing exceptional enzymatic performance over a broad temperature range. NPG Asia Mater 6:e114CrossRefGoogle Scholar
  19. Lin Y, Ren J, Qu X (2014b) Catalytically active nanomaterials: a promising candidate for artificial enzymes. Acc Chem Res 47(4):1097–1105. CrossRefGoogle Scholar
  20. Luo W, Zhu C, Su S, Li D, He Y, Huang Q, Fan C (2010) Self-catalyzed, self-limiting growth of glucose oxidase-mimicking gold nanoparticles. ACS Nano 4(12):7451–7458. CrossRefGoogle Scholar
  21. Manea F, Houillon FB, Pasquato L, Scrimin P (2004a) Nanozymes: gold-nanoparticle-based transphosphorylation catalysts. Angew Chem Int Ed Engl 43(45):6165–6169. CrossRefGoogle Scholar
  22. Manea F, Houillon FB, Pasquato L, Scrimin P (2004b) Nanozymes: gold-nanoparticle-based transphosphorylation catalysts. Angew Chem Int Ed 43:6165–6169CrossRefGoogle Scholar
  23. Mu J, Wang Y, Zhao M, Zhang L (2012) Intrinsic peroxidase-like activity and catalase-like activity of Co3O4 nanoparticles. Chem Commun 48(19):2540–2542. CrossRefGoogle Scholar
  24. Narayanan R, Lipert RJ, Porter MD (2008) Cetyltrimethylammonium bromide-modified spherical and cube-like gold nanoparticles as extrinsic Raman labels in surface-enhanced Raman spectroscopy based heterogeneous immunoassays. Anal Chem 80:2265–2271CrossRefGoogle Scholar
  25. Ni P, Dai H, Wang Y, Sun Y, Shi Y, Hu J, Li Z (2014) Visual detection of melamine based on the peroxidase-like activity enhancement of bare gold nanoparticles. Biosens Bioelectron 60:286–291. CrossRefGoogle Scholar
  26. Pengo P, Polizzi S, Pasquato L, Scrimin P (2005) Carboxylate-imidazole cooperativity in dipeptide-functionalized gold nanoparticles with esterase-like activity. J Am Chem Soc 127:1616–1617. CrossRefGoogle Scholar
  27. Pirmohamed T, Dowding JM, Singh S, Wasserman B, Heckert E, Karakoti AS, King JE, Seal S, Self WT (2010) Nanoceria exhibit redox state-dependent catalase mimetic activity. Chem Commun 46(16):2736–2738. CrossRefGoogle Scholar
  28. Shah J, Purohit R, Singh R, Karakoti AS, Singh S (2015) ATP-enhanced peroxidase-like activity of gold nanoparticles. J Colloid Interface Sci 456:100–107. CrossRefGoogle Scholar
  29. Sharma TK, Ramanathan R, Weerathunge P, Mohammadtaheri M, Daima HK, Shukla R, Bansal V (2014) Aptamer-mediated ‘turn-off/turn-on’ nanozyme activity of gold nanoparticles for kanamycin detection. Chem Commun 50(100):15856–15859. CrossRefGoogle Scholar
  30. Shi W, Wang Q, Long Y, Cheng Z, Chen S, Zheng H, Huang Y (2011) Carbon nanodots as peroxidase mimetics and their applications to glucose detection. Chem Commun 47(23):6695–6697. CrossRefGoogle Scholar
  31. Singh S, Pasricha R, Bhatta UM, Satyam P, Sastry M, Prasad B (2007) Effect of halogen addition to monolayer protected gold nanoparticles. J Mater Chem 17:1614–1619CrossRefGoogle Scholar
  32. Singh S, Dosani T, Karakoti AS, Kumar A, Seal S, Self WT (2011) A phosphate-dependent shift in redox state of cerium oxide nanoparticles and its effects on catalytic properties. Biomaterials 32(28):6745–6753. CrossRefGoogle Scholar
  33. Song Y, Qu K, Zhao C, Ren J, Qu X (2010a) Graphene oxide: intrinsic peroxidase catalytic activity and its application to glucose detection. Adv Mater 22(19):2206–2210. CrossRefGoogle Scholar
  34. Song Y, Wang X, Zhao C, Qu K, Ren J, Qu X (2010b) Label-free colorimetric detection of single nucleotide polymorphism by using single-walled carbon nanotube intrinsic peroxidase-like activity. Chemistry 16(12):3617–3621. CrossRefGoogle Scholar
  35. Stefan L, Denat F, Monchaud D (2012) Insights into how nucleotide supplements enhance the peroxidase-mimicking DNAzyme activity of the G-quadruplex/hemin system. Nucleic Acids Res 40(17):8759–8772. CrossRefGoogle Scholar
  36. Stiufiuc R, Iacovita C, Nicoara R, Stiufiuc G, Florea A, Achim M, Lucaciu CM (2013) One-step synthesis of pegylated gold nanoparticles with tunable surface charge. J Nanomater 2013:1–7CrossRefGoogle Scholar
  37. Turkevich J, Stevenson PC, Hillier J (1951) A study of the nucleation and growth processes in the synthesis of colloidal gold Farady Soc 11:55–75Google Scholar
  38. Vallabani NV, Karakoti AS, Singh S (2017) ATP-mediated intrinsic peroxidase-like activity of Fe3O4-based nanozyme: one step detection of blood glucose at physiological pH. Colloids Surf B Biointerfaces 153:52–60. CrossRefGoogle Scholar
  39. Wang S, Chen W, Liu AL, Hong L, Deng HH, Lin XH (2012) Comparison of the peroxidase-like activity of unmodified, amino-modified, and citrate-capped gold nanoparticles. ChemPhysChem 13:1199–1204. CrossRefGoogle Scholar
  40. Yang H, Xiao J, Su L, Feng T, Lv Q, Zhang X (2017) Oxidase-mimicking activity of the nitrogen-doped Fe3C@C composites. Chem Commun 53(27):3882–3885. CrossRefGoogle Scholar
  41. Zhan L, Li CM, Wu WB, Huang CZ (2014) A colorimetric immunoassay for respiratory syncytial virus detection based on gold nanoparticles-graphene oxide hybrids with mercury-enhanced peroxidase-like activity. Chem Commun 50(78):11526–11528. CrossRefGoogle Scholar
  42. Zheng X, Liu Q, Jing C, Li Y, Li D, Luo W, Wen Y, He Y, Huang Q, Long YT, Fan C (2011) Catalytic gold nanoparticles for nanoplasmonic detection of DNA hybridization. Angew Chem Int Ed Engl 50(50):11994–11998. CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Division of Biological and Life Sciences, School of Arts and SciencesAhmedabad UniversityAhmedabadIndia

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