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3 Biotech

, 8:85 | Cite as

Draft genome sequence of Paenibacillus strain LK1, a phytohormone producing bacterium

  • Kun Liu
  • Le He
  • Shiwei Li
  • Fengshou Tian
  • Zhongke Sun
  • Chengwei Li
Genome Letters

Abstract

A high-quality draft genome sequence of a multi-antibiotic resistant strain, Paenibacillus strain LK1 isolated from the rhizosphere of Auricularia auricular is described. Strain LK1 produced several types of phytohormones, which included Indole-3-acetic acid (IAA) and Zeatin. Phylogenetic analysis of the 16S rRNA gene of strain LK1 showed a close relationship to P. taichungensis and P. pabuli (99% gene sequence similarity). The genome size of strain LK1 was estimated to be 7.16 Mb that consisted of 82 contigs contained in 49 scaffolds. The G + C content of the genome was 45.63% and it encoded 6499 genes, 115 pseudo genes, 8 rRNAs, 33 tRNAs, and 4 ncRNAs, based on the Prokaryotic Genome Annotation Pipeline (PGAP). Further analysis of the genome of strain LK1 identified 16 genes encoding monooxygenase and six genes encoding cytochrome P450, which may be potentially involved in zeatin biosynthesis. The draft whole genome sequence and annotation of strain LK1 has been deposited at DDBJ/EMBL/GenBank under the accession number (PEII00000000).

Keywords

Paenibacillus sp NGS WGS Phytohormone Monooxygenase 

Notes

Acknowledgements

This work was partially supported by a grant from Zhoukou Normal University (no. ZKNU2015107) and a grant from the Education Department of Henan Province (no. 17A180017).

Compliance with ethical standards

Conflict of interest

We declare no conflict of interest with respect to this paper.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of Life Sciences and AgronomyZhoukou Normal UniversityZhoukouChina
  2. 2.Institute of Food and Drug InspectionZhoukou Normal UniversityZhoukouChina
  3. 3.Key Laboratory of Plant Genetics and Molecular BreedingZhoukou Normal UniversityZhoukouChina

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