3 Biotech

, 7:370 | Cite as

16S rDNA analysis of the effect of fecal microbiota transplantation on pulmonary and intestinal flora

  • Tianhao Liu
  • Zhongshan Yang
  • Xiaomei Zhang
  • Niping Han
  • Jiali Yuan
  • Yu ChengEmail author
Review Article


This study aims to explore the effect of FMT on regulations of dysbacteriosis of pulmonary and intestinal flora in rats with 16S rDNA sequencing technology. A total of 27 SPF rats (3–4 weeks old) were randomly divided into three groups: normal control group (K), model control group (MX), and fecal microbiota transplantation group (FMT); each group contained nine rats. The OTU values of the pulmonary and intestinal flora of the MX group decreased significantly compared with the normal control group. After FMT, the OTU value of pulmonary flora increased, while the value of OTU in intestinal flora declined. At the phylum level, FMT down-regulated Proteobacteria, Firmicutes, and Bacteroidetes in the pulmonary flora. At the genus level, FMT down-regulated Pseudomonas, Sphingobium, Lactobacillus, Rhizobium, and Acinetobacter, thus maintaining the balance of the pulmonary flora. Moreover, FMT could change the structure and diversity of the pulmonary and intestinal flora by positively regulating the pulmonary flora and negatively regulating intestinal flora. This study may provide a scientific basis for FMT treatment of respiratory diseases.


Fecal microbiota transplantation Pulmonary flora Intestinal flora 16S rDNA 



We appreciate the help provided by GENEWIZ, Inc. (Suzhou, China). At the same time, we thank the scholars who have provided relevant guidance for the study.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.


This study was supported by grants from the National Natural Science Foundation of China (81460684,81660765) and Yunnan Science and Technology Plan Project (2015FB194).


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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Tianhao Liu
    • 1
  • Zhongshan Yang
    • 1
  • Xiaomei Zhang
    • 1
  • Niping Han
    • 1
  • Jiali Yuan
    • 2
  • Yu Cheng
    • 1
    Email author
  1. 1.School of Basic Medical SciencesYunnan University of Traditional Chinese MedicineKunmingPeople’s Republic of China
  2. 2.Graduate SchoolYunnan University of Traditional Chinese MedicineKunmingPeople’s Republic of China

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