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3 Biotech

, 7:370 | Cite as

16S rDNA analysis of the effect of fecal microbiota transplantation on pulmonary and intestinal flora

  • Tianhao Liu
  • Zhongshan Yang
  • Xiaomei Zhang
  • Niping Han
  • Jiali Yuan
  • Yu ChengEmail author
Review Article

Abstract

This study aims to explore the effect of FMT on regulations of dysbacteriosis of pulmonary and intestinal flora in rats with 16S rDNA sequencing technology. A total of 27 SPF rats (3–4 weeks old) were randomly divided into three groups: normal control group (K), model control group (MX), and fecal microbiota transplantation group (FMT); each group contained nine rats. The OTU values of the pulmonary and intestinal flora of the MX group decreased significantly compared with the normal control group. After FMT, the OTU value of pulmonary flora increased, while the value of OTU in intestinal flora declined. At the phylum level, FMT down-regulated Proteobacteria, Firmicutes, and Bacteroidetes in the pulmonary flora. At the genus level, FMT down-regulated Pseudomonas, Sphingobium, Lactobacillus, Rhizobium, and Acinetobacter, thus maintaining the balance of the pulmonary flora. Moreover, FMT could change the structure and diversity of the pulmonary and intestinal flora by positively regulating the pulmonary flora and negatively regulating intestinal flora. This study may provide a scientific basis for FMT treatment of respiratory diseases.

Keywords

Fecal microbiota transplantation Pulmonary flora Intestinal flora 16S rDNA 

Notes

Acknowledgements

We appreciate the help provided by GENEWIZ, Inc. (Suzhou, China). At the same time, we thank the scholars who have provided relevant guidance for the study.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Funding

This study was supported by grants from the National Natural Science Foundation of China (81460684,81660765) and Yunnan Science and Technology Plan Project (2015FB194).

References

  1. Chakhava OV, Ruban SZ, Shustrova NM (1985) beta-Aspartylglycine during antibiotic induced dysbacteriosis of intestinal microflora in the rat. Prog Clin Biol Res 181:159–161Google Scholar
  2. Chakradhar S (2017) A curious connection: teasing apart the link between gut microbes and lung disease. Nat Med 23:402–404CrossRefGoogle Scholar
  3. Colman RJ, Rubin DT (2014) Fecal microbiota transplantation as therapy for inflammatory bowel disease: a systematic review and meta-analysis. J Crohn’s Colitis 8:1569–1581CrossRefGoogle Scholar
  4. Datta P, Gupta V, Mohi G K, Chander J (2017) Lactobacillus coryniformis Causing Pulmonary Infection in a Patient with Metastatic Small Cell Carcinoma: Case Report and Review of Literature on Lactobacillus Pleuro-Pulmonary Infections. Journal of Clinical & Diagnostic Research Jcdr 11:DE01–DE05Google Scholar
  5. De Leon LM, Watson JB, Kelly CR (2013) Transient flare of ulcerative colitis after fecal microbiota transplantation for recurrent Clostridium difficile infection. Clin Gastroenterol Hepatol Off Clin Pract J Am Gastroenterol Assoc 11:1036–1038Google Scholar
  6. Dickson RP, Erbdownward JR, Freeman CM, Walker N (2014) Changes in the lung microbiome following lung transplantation include the emergence of two distinct pseudomonas species with distinct clinical associations. PLoS ONE 9:e97214CrossRefGoogle Scholar
  7. Eiseman B, Silen W, Bascom GS, Kauvar AJ (1958) Fecal enema as an adjunct in the treatment of pseudomembranous enterocolitis. Surgery 44:854–859Google Scholar
  8. Herbert KM (2015) The gastrointestinal microbiome and diet-induced dysbiosis: influence on obesity and chronic disease risk. Dissertations Theses Gradworks 18:515–520Google Scholar
  9. Hu J, Qian M, Zhang Q, Cui J (2015) Sphingobium fuliginis HC3: a novel and robust isolated biphenyl- and polychlorinated biphenyls-degrading bacterium without dead-end intermediates accumulation. PLoS ONE 10:e0122740CrossRefGoogle Scholar
  10. Kelly CR, Kahn S, Kashyap P, Laine L (2015) Update on FMT 2015: indications, methodologies, mechanisms and outlook. Gastroenterology 149:223–237CrossRefGoogle Scholar
  11. Khoruts A, Dicksved J, Jansson JK, Sadowsky MJ (2010) Changes in the composition of the human fecal microbiome after bacteriotherapy for recurrent Clostridium difficile-associated diarrhea. J Clin Gastroenterol 44:354–360Google Scholar
  12. Li M, Liang P, Li Z, Wang Y (2015) Fecal microbiota transplantation and bacterial consortium transplantation have comparable effects on the re-establishment of mucosal barrier function in mice with intestinal dysbiosis. Front Microbiol 6:692Google Scholar
  13. Lo SC, Hung GC, Li B, Lei H (2015) Mixed group of Rhizobiales microbes in lung and blood of a patient with fatal pulmonary illness. Int J Clin Exp Pathol 8:13834–13852Google Scholar
  14. Marotz CA, Zarrinpar A (2016) Treating obesity and metabolic syndrome with fecal microbiota transplantation. Yale J Biol Med 89:383–388Google Scholar
  15. Mcknite AM, Perez-Munoz ME, Lu L, Williams EG (2012) Murine gut microbiota is defined by host genetics and modulates variation of metabolic traits. PLoS ONE 7:e39191CrossRefGoogle Scholar
  16. Michelle B, Goodrich JK, Jackson MA, Idil Y (2016) Heritable components of the human fecal microbiome are associated with visceral fat. Genome Biol 17:189CrossRefGoogle Scholar
  17. Paramsothy S, Walsh AJ, Borody T, Samuel D (2015) Gastroenterologist perceptions of faecal microbiota transplantation. World J Gastroenterol 21:10907–10914CrossRefGoogle Scholar
  18. Patel NC, Griesbach CL, Dibaise JK, Orenstein R (2013) Fecal microbiota transplant for recurrent Clostridium difficile infection: Mayo Clinic in Arizona experience. Mayo Clin Proc 88:799–805CrossRefGoogle Scholar
  19. Poroyko V, Meng F, Meliton A, Afonyushkin T (2015) Alterations of lung microbiota in a mouse model of LPS-induced lung injury. Am J Physiol Lung Cell Mol Physiol 309:L76–L83CrossRefGoogle Scholar
  20. Pragman AA, Kim HB, Reilly CS, Wendt C (2012) The lung microbiome in moderate and severe chronic obstructive pulmonary disease. PLoS ONE 7:e47305CrossRefGoogle Scholar
  21. Schwan A, Sjolin S, Trottestam U, Bo A (1983) Relapsing clostridium difficile enterocolitis cured by rectal infusion of homologous faeces. Lancet 2:845CrossRefGoogle Scholar
  22. Souto R, Silvaboghossian CM, Colombo AP (2014) Prevalence of Pseudomonas aeruginosa and Acinetobacter spp. in subgingival biofilm and saliva of subjects with chronic periodontal infection. Braz J Microbiol 45:495–501CrossRefGoogle Scholar
  23. Suresh BK, Kastelik J, Morjaria JB (2013) Role of long term antibiotics in chronic respiratory diseases. Respir Med 107:800–815CrossRefGoogle Scholar
  24. Tamburini S, Clemente JC (2017) Gut microbiota: neonatal gut microbiota induces lung immunity against pneumonia. Nat Rev Gastroenterol Hepatol 14:263–264CrossRefGoogle Scholar
  25. Tian H, Ge X, Nie Y, Yang L (2017) Fecal microbiota transplantation in patients with slow-transit constipation: a randomized. clinical trial. PLoS ONE 12:e0171308CrossRefGoogle Scholar
  26. Tsuei J, Chau T, Mills D, Wan Y-JY (2014) Bile acid dysregulation, gut dysbiosis, and gastrointestinal cancer. Exp Biol Med (Maywood, NJ) 239:1489–1504CrossRefGoogle Scholar
  27. Valverde JR, Mellado RP (2013) Analysis of metagenomic data containing high biodiversity levels. PLoS ONE 8:e58118CrossRefGoogle Scholar
  28. Van den Elsen LW, Poyntz HC, Weyrich LS, Young W, Forbes-Blom EE (2017) Embracing the gut microbiota: the new frontier for inflammatory and infectious diseases. Clin Transl Immunol 6(1):e125CrossRefGoogle Scholar
  29. Zoller V, Laguna AL, Prazeres DCO, Buch T (2015) Fecal microbiota transfer (FMT) in a patient with refractory irritable bowel syndrome. Dtsch Med Wochenschr 140:1232–1236CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Tianhao Liu
    • 1
  • Zhongshan Yang
    • 1
  • Xiaomei Zhang
    • 1
  • Niping Han
    • 1
  • Jiali Yuan
    • 2
  • Yu Cheng
    • 1
    Email author
  1. 1.School of Basic Medical SciencesYunnan University of Traditional Chinese MedicineKunmingPeople’s Republic of China
  2. 2.Graduate SchoolYunnan University of Traditional Chinese MedicineKunmingPeople’s Republic of China

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