3 Biotech

, 7:321 | Cite as

Application of customised bacterial inoculants for grass haylage production and its effectiveness on nutrient composition and fermentation quality of haylage

  • Ilavenil Soundharrajan
  • Da Hye Kim
  • Srigopalram Srisesharam
  • Palaniselvam Kuppusamy
  • Hyung Soo Park
  • Yong Hee Yoon
  • Won Ho Kim
  • Young Gil Song
  • Ki Choon ChoiEmail author
Original Article


The present study aimed to investigate the efficacy of customised Lactobacillus plantarum KCC-10, KCC-19 and K-46 on nutrient composition and fermentation quality of low moisture Italian ryegrass (IRG) forage. An addition of customised bacterial inoculants (CBI) did not affect the nutrient compositions and digestibility rates of haylage. The lactic acid content was higher in CBI-inoculated haylage, whereas the amount of acetic acid and butyric acid production was significantly reduced than the control. CBI-inoculated haylage exhibited higher numbers of bacterial colonies that reduced the pH of the haylage. Low pH in haylage is an important criterion for preventing undesirable microbial growth and improves fermentation quality of haylage. PCR studies indicated that the DNA of L. plantarum was predominantly amplified. It evidenced that the CBI is the main reason behind the improvement of haylage fermentation as compared to control. Overall results suggested that KCC-10, KCC-19 and K-46 are considered as potent strains for improving fermentation quality of low moisture forage and preserve its stability for a long time.


L. plantarum Italian ryegrass Low moisture forage Nutrient and digestibility rates Fermentation quality 



This work was carried out with the support of Cooperative Research Program for Agriculture Science and Technology Development (Project title: Isolation of lactic acid bacteria for manufacture of high quality silage and development of those utilization technologies; Project No. PJ00850201) Rural Development Administration, Republic of Korea. This study was supported by (2014) Postdoctoral Fellowship Program of (National Institute of Animal Science), Rural Development Administration, and Republic of Korea.

Compliance with ethical standards

Conflict of Interest

There is no conflict of interest.

Supplementary material

13205_2017_965_MOESM1_ESM.docx (13 kb)
Supplementary material 1 (DOCX 12 kb)


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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Ilavenil Soundharrajan
    • 1
  • Da Hye Kim
    • 2
  • Srigopalram Srisesharam
    • 1
  • Palaniselvam Kuppusamy
    • 1
  • Hyung Soo Park
    • 1
  • Yong Hee Yoon
    • 3
  • Won Ho Kim
    • 1
  • Young Gil Song
    • 4
  • Ki Choon Choi
    • 1
    Email author
  1. 1.Grassland and Forage DivisionNational Institute of Animal Science, Rural Development AdministrationCheonanRepublic of Korea
  2. 2.Laboratory of Animal Physiology, Graduate School of Agricultural ScienceTohoku UniversitySendaiJapan
  3. 3.Jungnong Bio IncGoksungRepublic of Korea
  4. 4.Pyung Hwa Dairy Farm, Young GwangChunnamRepublic of Korea

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