3 Biotech

, 7:206 | Cite as

Optimization of pectinase-assisted and tri-solvent-mediated extraction and recovery of lycopene from waste tomato peels

  • Pravin J. Munde
  • Abhijeet B. Muley
  • Mayur R. Ladole
  • Amesh V. Pawar
  • Mohammed I. Talib
  • Vishal R. Parate
Original Article


In the present work, optimization of pectinase-assisted and tri-solvent-mediated extraction of lycopene from waste tomato peels was carried out. The optimized parameters for enzymatic pre-treatment were 2% pectinase concentration, pH 5.5, 4-h incubation, 45 °C and 150 rpm. Maximum recovery of lycopene from tomato peels using optimized tri-solvent extraction was achieved at 45 °C, 120-min incubation and 200 rpm. The extracted lycopene was confirmed through functional and characteristic peaks in UV–Vis and FTIR spectra and with retention time in HPLC. The radical scavenging activity was 72.30 ± 2.70 and 43.40 ± 2.01 µg ascorbic acid equivalents (AAE)/ml for 1,1-diphenyl-2-picrylhydrzyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radicals, respectively. The optimized method resulted in 7.38, 4.65 and 1.59 times enhancement in lycopene extraction and recovery in correlation with single solvent, enzyme-treated and tri-solvent extraction methods, respectively.


Lycopene Tomato peels Antioxidant Pectinase Tri-solvent extraction 



The authors would like to thank the Department of Food Technology, University Institute of Chemical Technology, North Maharashtra University, Jalgaon, India for availing all the required facilities and financial support to carry out this research work.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest related to this work.


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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Pravin J. Munde
    • 1
  • Abhijeet B. Muley
    • 1
  • Mayur R. Ladole
    • 1
  • Amesh V. Pawar
    • 1
  • Mohammed I. Talib
    • 1
  • Vishal R. Parate
    • 1
  1. 1.Department of Food Technology, University Institute of Chemical TechnologyNorth Maharashtra UniversityJalgaonIndia

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