Chicken egg shell as a potential substrate for production of alkaline protease by Bacillus altitudinis GVC11 and its applications
Abstract
Chicken egg shell and membrane were used as substrate for production of alkaline protease by Bacillus altitudinis GVC11. Maltose as additional carbon source enhanced enzyme production up to 13%. Addition of organic nitrogen sources like peptone and yeast extract increased enzyme production by 9% and 5%, respectively and inorganic nitrogen sources did not have any positive effect. The resultant protein hydrolyzate after fermentation was found to have essential amino acids such as leucine, phenyl alanine, isoleucine, lysine, valine, methionine, arginine in considerable quantities and minute concentrations of cysteine. The protein hydrolyzate was also found to have good antioxidant activity.
Keywords
Alkaline protease Egg shell Collagen Bioactive compounds Bacillus altitudinis GVC11Notes
Acknowledgements
The authors thank the DBT- ISLARE for financial support to carry out this work and fellowship to HK.
Compliance with ethical standards
Conflict of interest
The authors have no conflict of interest for this research work.
References
- Cheng M, Takenaka S, Aoki S, Murakami S, Aoki K (2009) Purification and characterization of an eggshell membrane decomposing protease from Pseudomonas aeruginosa strain ME-4. J Biosci Bio Eng 107:373–378. doi: 10.1016/j.jbiosc.2008.12.010 CrossRefGoogle Scholar
- Ibrahim ASS, Salamah A, Elbadawi YB, Tayeb ME, Ibrahim SSS (2015) Production of extracellular alkaline protease by new halotolerant alkaliphilic Bacillus sp. NPST-AK15 isolated from hyper saline soda lakes. Electron J Biotechnol 18:236–243. doi: 10.1016/j.ejbt.2015.04.001 CrossRefGoogle Scholar
- Kingori AM (2011) A review of the uses of poultry egg shell and shell membranes. Int J Poult Sci 10(11):908–912. doi: 10.3923/ijps.2011.908.912 CrossRefGoogle Scholar
- Korhonen H, Pihlanto A (2003) Food-derived bioactive peptides—opportunities for designing future foods. Curr Pharm Des 9(16):1297–1308. doi: 10.2174/1381612033454892 CrossRefGoogle Scholar
- Memarpoor-Yazdi M, Asoodeh A, Chamani J (2012) A novel antioxidant and antimicrobial peptide from hen egg white lysozyme hydrolyzates. J Funct Foods 4:278–286. doi: 10.1016/j.jff.2011.12.004 CrossRefGoogle Scholar
- Mukherjee AK, Adhikari H, Rai SK (2008) Production of alkaline protease by a thermophilic Bacillus subtilis under solid-state fermentation (SSF) condition using Imperatacylindrica grass and potato peel as low-cost medium: characterization and application of enzyme in detergent formulation. Biochem Eng J 39:353–361. doi: 10.1016/j.bej.2007.09.017 CrossRefGoogle Scholar
- Nakano T, Ikawa NI, Ozimek L (2003) Chemical composition of chicken egg shell and shell membranes. Poult Sci 82:510–514CrossRefGoogle Scholar
- Nilegaonkar SS, Zambare VP, Kanekar PP, Dhakephalkar PK, Sarnaik SS (2007) Production and partial characterization of dehairing protease from Bacillus cereus MCM B-326. Bioresour Technol 98:1238–1245. doi: 10.1016/j.biortech.2006.05.003 CrossRefGoogle Scholar
- Nimalaratne C, Bandara N, Wu J (2015) Purification and characterization of antioxidant peptides from enzymatically hydrolyzed chicken egg white. Food Chem 188:467–472. doi: 10.1016/j.foodchem.2015.05.014 CrossRefGoogle Scholar
- Pant G, Prakash A, Pavani JVP, Bera S, Deviram GVNS, Kumar Ajay, Panchpuri M, Prasuna RG (2015) Production, optimization and partial purification of protease from Bacillus subtilis. J Taibah Univ Sci 9:50–55. doi: 10.1016/j.jtusci.2014.04.010 CrossRefGoogle Scholar
- Phil G, Zhihong M (2009) High value products from hatchery waste. RIRDC 09/061Google Scholar
- Sanathan PT, Lomate PR, Giri AP, Hivrale VK (2013) Characterization of a chemostable serine alkaline protease from Periplaneta Americana. BMC Biochem 14:1–9. doi: 10.1186/1471-2091-14-32 CrossRefGoogle Scholar
- Shahidi F, Zhong Y (2008) Bioactive peptides. J AOAC Int 91:914–931Google Scholar
- Shi Z, Li H, Li Z, Hu J, Zhang H (2013) Pre-column derivatization RP-HPLC determination of amino acids in Asparagi radix before and after heating process. In: Proceedings of 5 International conference on agricultural and natural resources engineering, Baoding, pp 351–356. doi: 10.1016/j.ieri.2013.11.115
- Shi Y, Nolan JK, Jiang B, Tsao R, Mine Y (2014) Antioxidant activity of enzymatic hydrolyzates from egg shell membrane proteins and its protective capacity in human intestinal epithelial CaCo2 cells. J Funct Foods 10:35–45. doi: 10.1016/j.jff.2014.05.004 CrossRefGoogle Scholar
- Srividya SM, Mala M (2011) Influence of process parameters on the production of detergent compatible alkaline protease by a newly isolated Bacillus sp. Y. Turk J Biol 35:177–182. doi: 10.3906/biy-0906-47 Google Scholar
- Verma N, Kumar Vivek, Bansal MC (2012) Utilisation of egg shell waste in cellulase production by Neurosporacrassa under wheat bran based solid state fermentation. Pol J Environ Stud 21(2):491–497Google Scholar
- Vijay Kumar E, Srijana M, Chitanya K, Harish Kumar Reddy Y, Reddy Gopal (2011a) Biodegradation of poultry feathers by a novel bacterial isolate Bacillus altitudinis GVC11. Ind J Biotechnol 10:502–507Google Scholar
- Vijay Kumar E, Srijana M, Kiran Kumar K, Harikrishna N, Reddy Gopal (2011b) A novel serine alkaline protease from Bacillus altitudinis GVC11and its application as a dehairing agent. Bioprocess Biosyst Eng 34:403–409. doi: 10.1007/s00449-010-0483-x CrossRefGoogle Scholar
- Vijayaraghavan P, Lazarus S, Vincent SGP (2014) De-hairing protease production by an isolated Bacillus cereus strain AT under solid-state fermentation using cow dung: biosynthesis and properties. Saudi J Biol Sci 21:27–34. doi: 10.1016/j.sjbs.2013.04.010 CrossRefGoogle Scholar
- Wong M, Hendrix MJC, Mark K, Little C, Stem R (1984) Collagen in the egg shell membranes of the hen. Dev Biol 104:28–36CrossRefGoogle Scholar
- Zhao YH, Chi YJ (2009) Characterization of collagen from eggshell membrane. Biotechnol 8(2):254–258CrossRefGoogle Scholar
Copyright information
© Springer-Verlag GmbH Germany 2017