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3 Biotech

, 7:76 | Cite as

Total phenolics, antioxidant, antitumor, and enzyme inhibitory activity of Indian medicinal and aromatic plants extracted with different extraction methods

  • Shivraj Hariram NileEmail author
  • Arti Shivraj Nile
  • Young-Soo Keum
Original Article

Abstract

The phenolic content, antioxidant, antitumor, and enzyme inhibitory activities of commonly used medicinal herbs from a Unani system of medicine were investigated using four different extraction methods. Among the plants studied, the Hyssopus officinalis L, Origanum vulgare L, and Portulaca oleracea L. extracts showed the highest amount of total phenolics (64.40, 60.35, and 58.81 mg GAE/g) and revealed significant antioxidant activities. The plants also showed a maximum cytotoxic activity as indicated by H. officinalis (82%), O. vulgare (75%), and P. oleracea (72%) showed more than 70% cytotoxicity for breast cancer cells, 82% of the cells were dead at the concentration of 500 mg/mL. The plants H. officinalis, P. oleracea, O. vulgare, and Rubia cordifolia L. revealed more than 80% inhibition towards xanthine oxidase and comprising maximum 70% of inhibition for superoxide dismutase. From results we conclude that there is a strong correlation between phenolic content, antioxidant, and enzyme inhibitory activity among these plants, indicating phenolics are the major compounds for these biological activities. Furthermore, this study provides the basis for the therapeutic importance of studied plants as latent inhibitors of oxidative stress and antitumor cell proliferation which correlate with the ethnobotanical data contained in the Unani system of medicine.

Keywords

Unani medicine Total phenolics Antioxidant Xanthine oxidase Antitumor 

Notes

Acknowledgements

This research was supported by KU-Research Professor Program-2017, Konkuk University, Seoul, Republic of Korea.

Compliance with ethical standards

Conflict of interest

There is no conflict of interest.

References

  1. Amarowicz R, Pegg RB, Rahimi-Moghaddam P, Barl B, Weil JA (2004) Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies. Food Chem 84(4):551–562CrossRefGoogle Scholar
  2. Andrade D, Gil C, Breitenfeld L, Domingues F, Duarte AP (2009) Bioactive extracts from Cistus ladanifer and Arbutus unedo L. Ind Crops Prod 30:165–167CrossRefGoogle Scholar
  3. Ansari AA (1985) Prospects of Unani System of Medicine in Primary Health Care in India. Indian J Hosp Adm New Delhi XXII(1–2):223–225Google Scholar
  4. Aspé E, Fernández K (2011) The effect of different extraction techniques on extraction yield, total phenolic, and anti-radical capacity of extracts from Pinus radiata Bark. Ind Crops Prod 34:838–844CrossRefGoogle Scholar
  5. Cai YZ, Sun M, Corke H (2003) Antioxidant activity of betalains from plants of the Amaranthaceae. J Agric Food Chem 51(8):2288–2294CrossRefGoogle Scholar
  6. Cai YZ, Luo Q, Sun M, Corke H (2004) Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci 74(17):2157–2184CrossRefGoogle Scholar
  7. Dhanani T, Shah S, Gajbhiye N, Kumar S (2017) Effect of extraction methods on yield, phytochemical constituents and antioxidant activity of Withania somnifera. Arab J Chem 10:S1193–S1199CrossRefGoogle Scholar
  8. Dudonne S, Vitrac X, Coutiere P, Woillez M, Merillon JM (2009) Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD, and ORAC assays. J Agric Food Chem 57(5):1768–1774CrossRefGoogle Scholar
  9. Ekor M (2014) The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Front Pharmacol 4(177):1–10Google Scholar
  10. Ferreira A, Proença C, Serralheiro MLM, Araujo MEM (2006) The in vitro screening for acethylcholinesterase inhibition and antioxidant activity of medicinal plants from Portugal. J Ethnopharmacol 108:31–37CrossRefGoogle Scholar
  11. Hayouni EA, Abedrabba M, Bouix M, Hamdi M (2007) The effects of solvents and extraction method on the phenolic contents and biological activities in vitro of Tunisian Quercus coccifera L. and Juniperus phoenica L. fruit extracts. Food Chem 105:1126–1134CrossRefGoogle Scholar
  12. Husein AI, Ali-Shtayeh MS, Jondi WJ, Zatar NA, Abu-Reidah IM, Jamous RM (2014) In vitro antioxidant and antitumor activities of six selected plants used in the Traditional Arabic Palestinian herbal medicine. Pharm Biol 52:1249–1255CrossRefGoogle Scholar
  13. Izhar N (1989) The Unani traditional medical system in India: a case study in health behavior. Geographia Medica 19:163–185Google Scholar
  14. Karabegovic IT, Stojičević SS, Veličković DT, Todorović ZB, Nikolić NČ, Lazić ML (2014) The effect of different extraction techniques on the composition and antioxidant activity of cherry laurel (Prunus laurocerasus) leaf and fruit extracts. Ind Crops Prod 54(2):142–148CrossRefGoogle Scholar
  15. Khanna-Chopra R, Semwal VK (2011) Superoxide dismutase and ascorbate peroxidase are constitutively more thermotolerant than other antioxidant enzymes in Chenopodium album. Physiol Mol Biol Plants 17(4):339–346CrossRefGoogle Scholar
  16. Kosar M, Demirci B, Demirci F, Baser KHC (2008) Effect of maturation on the composition and biological activity of the essential oil of a commercially important Satureja species from Turkey: satureja cuneifolia Ten. J Agric Food Chem 56:2260–2265CrossRefGoogle Scholar
  17. Li H, Pordesimo L, Weiss J (2004) High intensity ultrasound: assisted extraction of oil from soybeans. Food Res Int 37:731–738CrossRefGoogle Scholar
  18. Mraihi M, Journi M, Chérif JK, Sokmen M, Sokmen A, Ayadi MT (2013) Phenolic contents and antioxidant potential of Crataegus fruits grown in Tunisia as determined by DPPH, FRAP, and β-carotene/linoleic acid assay. J Chem Article ID 378264:6. doi: 10.1155/2013/378264 Google Scholar
  19. Nile SH, Khobragade CN (2011) In vitro anti-inflammatory and xanthine oxidase inhibitory activity of Tephrosia purpurea shoot extract. Nat Prod Commun 6:1437–1440Google Scholar
  20. Nile SH, Park SW (2013) Total phenolics, antioxidant and xanthine oxidase inhibitory activity of three colored onions (Allium cepa L.). Front Life Sci 7:224–228CrossRefGoogle Scholar
  21. Nile SH, Park SW (2014) Antioxidant: α-glucosidase and xanthine oxidase inhibitory activity of bioactive compounds from maize (Zea mays L.). Chem Biol Drug Des 83:119–125CrossRefGoogle Scholar
  22. Nile SH, Park SW (2015) Chromatographic analysis, antioxidant, anti-inflammatory, and xanthine oxidase inhibitory activities of ginger extracts and its reference compounds. Ind Crop Prod 70:238–244CrossRefGoogle Scholar
  23. Pandey MM, Rastogi S, Rawat AKS (2013) Indian traditional ayurvedic system of medicine and nutritional supplementation. Evid Based Complement Alternat Med 2013:376327Google Scholar
  24. Pietta PG (2000) Flavonoids as antioxidants. J Nat Prod 63:1035–1042CrossRefGoogle Scholar
  25. Quispe-Candori S, Foglio MA, Rosa PTV, Meireles MAA (2008) Obtaining β-caryophyllene from Cordia verbenacea de Candolle by super critical fluid extraction. J Supercrit Fluids 46:27–32CrossRefGoogle Scholar
  26. Ravishankar B, Shukla VJ (2007) Indian System of Medicine: a brief profile. Afr J Tradit Complement Altern Med 4(3):319–337CrossRefGoogle Scholar
  27. Samal J (2016) Medicinal plants and related developments in India: a peep into 5-year plans of India. Ind. J Health Sci 9:14–19CrossRefGoogle Scholar
  28. Samaradivakara SP, Samarasekera R, Handunnetti SM, Weerasena OVDSJ (2016) Cholinesterase, protease inhibitory and antioxidant capacities of Sri Lankan medicinal plants. Ind Crops Prod 83:227–234CrossRefGoogle Scholar
  29. Senthilraja P, Kathiresan K (2015) In vitro cytotoxicity MTT assay in Vero, HepG2 and MCF -7 cell lines study of Marine Yeast. J App Pharm Sci 5(3):080–084CrossRefGoogle Scholar
  30. Shoemaker M, Cohen I, Campbell M (2004) Reduction of MTT by aqueous herbal extracts in the absence of cells. J Ethnopharmacol 93:381–384CrossRefGoogle Scholar
  31. Shui G, Leong LP (2002) Separation and determination of organic acids and phenolic compounds in fruit juices and drinks by high-performance liquid chromatography. J Chromatogr A 977:89–96CrossRefGoogle Scholar
  32. Skotti E, Anastasaki E, Kanellou G, Polissiou M, Tarantilis PA (2014) Total phenolic content, antioxidant activity and toxicity of aqueous extracts from selected Greek medicinal and aromatic plants. Ind Crops Prod 53:46–54CrossRefGoogle Scholar
  33. Stalikas CD (2007) Extraction, separation, and detection methods for phenolic acids and flavonoids. J Sep Sci 30:3268–3295CrossRefGoogle Scholar
  34. Surveswaran S, Cai YZ, Corke H, Sun M (2007) Systematic evaluation of natural phenolic antioxidants from 133 Indian medicinal plants. Food Chem 102:938–953CrossRefGoogle Scholar
  35. Tajkarimi MM, Ibrahim SA, Cliver DO (2010) Antimicrobial herb and spice compounds in food. Food Control 21:1199–1218CrossRefGoogle Scholar
  36. Terigar BG, Balasubramanian S, Sabliov CM, Lima M, Boldor D (2011) Soybean and rice bran oil extraction in a continuous microwave system: from laboratory to pilot scale. J Food Eng 104:208–217CrossRefGoogle Scholar
  37. Wang B, Deng J, GaoY ZhuL, He R, Xu Y (2011) The screening toolbox of bioactive substances from natural products: a review. Fitoterapia 82:1141–1151CrossRefGoogle Scholar
  38. Zhang SQ, Bi HM, Liu CJ (2007) Extraction of bio-active components from Rhodiola sachalinensis under ultrahigh hydrostatic pressure. Sep Purif Technol 57:277–282CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Shivraj Hariram Nile
    • 1
    Email author
  • Arti Shivraj Nile
    • 1
  • Young-Soo Keum
    • 1
  1. 1.Department of Bio-resources and Food Science, College of Life and Environmental SciencesKonkuk UniversitySeoulSouth Korea

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