3 Biotech

, 7:76 | Cite as

Total phenolics, antioxidant, antitumor, and enzyme inhibitory activity of Indian medicinal and aromatic plants extracted with different extraction methods

  • Shivraj Hariram NileEmail author
  • Arti Shivraj Nile
  • Young-Soo Keum
Original Article


The phenolic content, antioxidant, antitumor, and enzyme inhibitory activities of commonly used medicinal herbs from a Unani system of medicine were investigated using four different extraction methods. Among the plants studied, the Hyssopus officinalis L, Origanum vulgare L, and Portulaca oleracea L. extracts showed the highest amount of total phenolics (64.40, 60.35, and 58.81 mg GAE/g) and revealed significant antioxidant activities. The plants also showed a maximum cytotoxic activity as indicated by H. officinalis (82%), O. vulgare (75%), and P. oleracea (72%) showed more than 70% cytotoxicity for breast cancer cells, 82% of the cells were dead at the concentration of 500 mg/mL. The plants H. officinalis, P. oleracea, O. vulgare, and Rubia cordifolia L. revealed more than 80% inhibition towards xanthine oxidase and comprising maximum 70% of inhibition for superoxide dismutase. From results we conclude that there is a strong correlation between phenolic content, antioxidant, and enzyme inhibitory activity among these plants, indicating phenolics are the major compounds for these biological activities. Furthermore, this study provides the basis for the therapeutic importance of studied plants as latent inhibitors of oxidative stress and antitumor cell proliferation which correlate with the ethnobotanical data contained in the Unani system of medicine.


Unani medicine Total phenolics Antioxidant Xanthine oxidase Antitumor 



This research was supported by KU-Research Professor Program-2017, Konkuk University, Seoul, Republic of Korea.

Compliance with ethical standards

Conflict of interest

There is no conflict of interest.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Shivraj Hariram Nile
    • 1
    Email author
  • Arti Shivraj Nile
    • 1
  • Young-Soo Keum
    • 1
  1. 1.Department of Bio-resources and Food Science, College of Life and Environmental SciencesKonkuk UniversitySeoulSouth Korea

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