Facile synthesis, characterization of nano-tungsten trioxide decorated with silver nanoparticles and their antibacterial activity against water-borne gram-negative pathogens

  • Umair Baig
  • M. A. GondalEmail author
  • Suriya Rehman
  • Sultan Akhtar
Original Article


In the present work, silver decorated tungsten trioxide (WO3) metal-oxide semiconducting nanoparticles were synthesized using a facile wet impregnation technique for inactivation of harmful water-borne gram-negative pathogens. The structural and morphological properties of self-synthesized pure WO3 nanoparticles (n-WO3) and different weight percentage of Ag metal (3 and 5%) loaded WO3 (3% n-Ag/WO3, 5% n-Ag/WO3) were analyzed by XRD, SEM, TEM and SAED. The loading of Ag on n-WO3 was confirmed by TEM and SAED analysis. The chemical composition of as-synthesized n-WO3, 3% n-Ag/WO3 and 5% n-Ag/WO3 was identified by XPS. The antibacterial characterization and morphogenic studies were done on the following water borne bacteria: Shigella flexneri, Salmonella cholerasis and Escherichia coli. Morphogenesis by electron microscopy revealed the close contact of nanomaterials with the bacterial cell wall and membrane leading to severe damage to the integrity of membrane which caused cell death.


Nanomaterial Ag decorated Tungsten trioxide Antibacterial activity Morphogenesis 



The authors would like to thank DSR and CoRE-DWT at King Fahd University of Petroleum and Minerals, Saudi Arabia for funding to support this work under project # DISC1504.

Compliance with ethical standards

Conflict of interest

On behalf of all authors, the corresponding author states that there is no conflict of interest.

Supplementary material

13204_2019_1186_MOESM1_ESM.docx (16 kb)
Supplementary material 1 (DOCX 15 kb)


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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  • Umair Baig
    • 1
  • M. A. Gondal
    • 2
    Email author
  • Suriya Rehman
    • 3
  • Sultan Akhtar
    • 4
  1. 1.Center for Research Excellence in Desalination and Water TreatmentKing Fahd University of Petroleum and MineralsDhahranSaudi Arabia
  2. 2.Department of Physics and Center for Research Excellence in NanotechnologyKing Fahd University of Petroleum and MineralsDhahranSaudi Arabia
  3. 3.Department of Epidemic Disease Research, Institute for Research and Medical Consultations (IRMC)Imam Abdulrahman Bin Faisal University (IAU)DammamSaudi Arabia
  4. 4.Department of Biophysics, Institute for Research and Medical Consultations (IRMC)Imam Abdulrahman Bin Faisal University (IAU)DammamSaudi Arabia

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