DNase-I functionalization of ciprofloxacin-loaded chitosan nanoparticles overcomes the biofilm-mediated resistance of Pseudomonas aeruginosa

  • Krishna Kumar Patel
  • Ashish Kumar AgrawalEmail author
  • Md. Meraj Anjum
  • Muktanand Tripathi
  • Nidhi Pandey
  • Sankha Bhattacharya
  • Ragini Tilak
  • Sanjay SinghEmail author
Original Article


Pseudomonas aeruginosa infection in cystic fibrosis (CF) is a major complication which aggravates the disease complexity and progression. The improvement of antimicrobial therapy against the P. aeruginosa biofilm infection in CF by dissembling the extracellular matrix is a fundamental concept of this study. Chitosan nanoparticle was successfully fabricated and characterized. Furthermore, various in vitro antimicrobial efficacy, biofilm dispersal potential, and in vitro as well as in vivo toxicity were assessed. The chitosan nanoparticles of ciprofloxacin functionalized with DNase-I had spherical shape with desired quality attributes (particle size—212.3 ± 8.9; polydispersity index—0.288 ± 0.06, zeta potential—14.6 ± 1.3, and entrapment efficiency—53.7 ± 3.8%). The developed DNase-I functionalized chitosan nanoparticles laden with ciprofloxacin demonstrated substantial and prolonged microbial inhibition, efficiently prevented the biofilm development, and possessed the excellent biofilm dispersal potential. Moreover, the confocal study demonstrated that the biofilm treated with DNase-I functionalized chitosan nanoparticles of ciprofloxacin had minimum biofilm thickness, biomass, and microbial density compared to the other treatment groups. Additionally, the developed formulation was found to be safe and had minimal in vitro as well as in vivo toxicity. Thus, the findings of this study suggests ciprofloxacin-loaded chitosan nanoparticles functionalized with DNase-I as an effective and safe treatment approach for the P. aeruginosa infection in CF.


Chitosan nanoparticles DNase-I Cystic fibrosis Ciprofloxacin Biofilm 



The authors are grateful to Indian Institute of Technology (Banaras Hindu University), Varanasi, for rendering the support in terms of infrastructure, facilities, and research support grant to complete the work on time. We also thank the Central Instrument Facility Centre, IIT (BHU), Varanasi for extending the facilities when needed. We also acknowledge the Interdisciplinary school of life sciences, Institute of Science, Banaras Hindu University, Varanasi for confocal microscopy. Moreover, we are also thankful to Ministry of Human Resources and Development, Government of India for offering Mr. K.K. Patel teaching assistantship to complete his Ph.D.

Compliance with ethical standards

Conflict of interest

Authors have no conflict of interest.


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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  • Krishna Kumar Patel
    • 1
  • Ashish Kumar Agrawal
    • 1
    Email author
  • Md. Meraj Anjum
    • 1
  • Muktanand Tripathi
    • 2
  • Nidhi Pandey
    • 2
  • Sankha Bhattacharya
    • 1
  • Ragini Tilak
    • 2
  • Sanjay Singh
    • 1
    Email author
  1. 1.Department of Pharmaceutical Engineering and TechnologyIndian Institute of Technology (IIT-BHU)VaranasiIndia
  2. 2.Department of Microbiology, Institute of Medical SciencesBanaras Hindu UniversityVaranasiIndia

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