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Co-immobilization of multiple enzymes onto surface-functionalized magnetic nanoparticle for the simultaneous hydrolysis of multiple substrates containing industrial wastes

  • S. Hepziba Suganthi
  • K. V. Swathi
  • Raagini Biswas
  • Sneha Basker
  • K. RamaniEmail author
Original Article
  • 4 Downloads

Abstract

The present study focused on the co-immobilization of multi-enzymes onto magnetic nanoparticles (MNPs) and surface-functionalized MNPs for the hydrolysis of fish processing solid waste (FPSW) containing complex molecules. The MNPs were synthesized and surface-functionalized by chemical (cMNP and SF-cMNP) and biological method [biologically synthesized iron oxide magnetic nanoparticle (bIOMNP)] and was characterized by various instrumental analyses. Lipase and protease produced from Streptomyces thermolineatus were co-immobilized onto the synthesized bare and surface-functionalized MNPs. At the optimum conditions, the lipase and protease loading capacity of cMNP were found as 1000 and 783 U/g, respectively, whereas the lipase and protease loading capacity of SF-cMNP were 2920 and 2350 U/g, respectively, and bIOMNP was 2800 and 2196 U/g, respectively. The thermal, pH and storage stabilities of the co-immobilized enzymes were greatly enhanced compared to the free enzymes and the Vmax and Km of the free and co-immobilized enzymes were also determined. The free and the co-immobilized enzymes were used for the hydrolysis of lipids and proteins in FPSW. The percentage hydrolysis of lipids and proteins in FPSW was 52.8 and 61.8%, respectively, for LP-cMNP, 76.2 and 84.1%, respectively, for LP-SF-cMNP and 73.9 and 82.1%, respectively, for LP-bIOMNP.

Keywords

Fish processing solid waste Iron oxide magnetic nanoparticles Surface functionalization Enzyme co-immobilization Multi-enzyme catalysis 

Notes

Acknowledgements

The authors are grateful to the Department of Biotechnology, Nanotechnology research centre, Department of Physics & Nanotechnology, SRM Institute of Science and Technology and SAIF, IIT-Madras for extending the analytical facilities.

Compliance with ethical standards

Conflict of interest

None.

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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  • S. Hepziba Suganthi
    • 1
  • K. V. Swathi
    • 1
  • Raagini Biswas
    • 1
  • Sneha Basker
    • 1
  • K. Ramani
    • 1
    Email author
  1. 1.Biomolecules and Biocatalysis Laboratory, Department of BiotechnologySRM Institute of Science and TechnologyKattankulathurIndia

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