Nanoliposomal system of rosemary essential oil made by specific human cell phospholipids and evaluation of its anti-cancer properties

  • Shahrzad SalariEmail author
  • Reza Salari
Original Article


Herbal extracts including herbal essential oils typically have lesser side effects when compared to synthetic chemical drugs. Their use in free form is limited due to their chemical instability and sensitivity to oxidation. One approach to improving their stability and delivery is to encapsulate the active herbal compounds in lipid carriers such as nanoliposomes. Rosemary (Rosmarinus officinalis L.) is a common herbal medicine with useful antioxidant and antimicrobial effects. In this study, the rosemary essential oil was first extracted and its compounds were characterized using gas chromatography and mass spectrometry (GC–MS). Then nanoliposomal carriers were constructed using both sonication and filtration techniques and their size and release pattern were evaluated. Finally, the anti-cancer properties of the free and encapsulated rosemary essential oil on MCF7 cell line (Michigan Cancer Foundation-7) were evaluated. Our results show that the liposomal essential oil has higher toxic effects on the MCF7 cell line due to improved drug delivery.


Nanoliposomes Drug delivery Gas chromatography and mass spectrometry (GC–MS) Rosemary (Rosmarinus officinalis L.) Essential oil 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. Anderson LJE (2010) optically guided controlled release from liposomes with tunable plasmonic nanobubbles. J Controlled Release 144(2):151–158CrossRefGoogle Scholar
  2. Anonymous A (2010) Scientific opinion of the panel on the use of oregano and lemon balm extracts as a food additive. EFSA Journal 8(2):1514–1524Google Scholar
  3. Arias JL (2014) Nanotechnology and drug delivery. Nanoplatforms in drug delivery. CRC Press, USACrossRefGoogle Scholar
  4. Bangham AD (1992) Liposomes: realizing their promise. Hosp Pract (Off Ed) 27(12):51–61CrossRefGoogle Scholar
  5. Begum K, Sarker A, Shimu IJ, Chowdhury MMI, Jalil R (2016) Characterization of nanoemulsion prepared from self-emulsifying rifampicin and its antibacterial effect on staphylococcus aureus and stap. Epidermidis isolated from AcneDhaka Uni J Pharm SciGoogle Scholar
  6. Benita S (2006) Microencapsulation Methods and Industrial Applications, 2nd edn. CRC Press, USA, pp 44–55Google Scholar
  7. Brannon-Peppas L, Blanchette JO (2004) Nanoparticle and targeted systems for cancer therapy. Adv Drug Deliv Rev 56(11):1649–1659CrossRefGoogle Scholar
  8. Burt S (2004) Essential oils, their antibacterial properties and potential applications in foods: a review. Int J Food Microbiol 94(3):223–233CrossRefGoogle Scholar
  9. Celia C, Trapasso E, Locatelli M, Navarra M, Ventura CA, Wolfram J, Anticancer activity of liposomal bergamot essential oil (BEO) on human neuroblastoma cells. Colloids Surfaces B Biointerfaces, 2013Google Scholar
  10. Duncan R, Polymer-drug conjugates: towards a novel approach for the treatment of endrocine-related cancer. EndocrRelat Cancer, 2005; 189 – 99Google Scholar
  11. Gu FX (2007) Targeted nanoparticles for cancer therapy. Nano Today 2(3):14–21CrossRefGoogle Scholar
  12. Haley B, Frenkel E (2008) Nanoparticles for Drug Delivery in Cancer Treatment. Urologic Oncology: Seminars Original Investigations 26:57–64CrossRefGoogle Scholar
  13. Kubo T (2000) Targeted delivery of anticancer drugs with intravenously administered magnetic liposomes in osteosarcoma-bearing hamsters. Int J Oncol 17(2):309–315Google Scholar
  14. Minko T (2006) New generation of liposomal drugs for cancer. Anti-Cancer Agents Med Chem 6(6):537–549CrossRefGoogle Scholar
  15. Nedovic V, Kalusevic A, Manojlovic V, Levic S, Bugarski B (2011) An overview of encapsulation technologies for food applications. Procedia Food Sci 1:1806–1815CrossRefGoogle Scholar
  16. Oluwatuyi M, Kaatz GW, Gibbons S (2004) Antibacterial and resistance modifying activity of Rosmarinus officinalis. Phytochem 65(24):3249–3254CrossRefGoogle Scholar
  17. Peer D (2007) Nanocarriers as an emerging platform for cancer therapy. Nat Nano 2(12):751–760CrossRefGoogle Scholar
  18. Singh K, Mezei M (1984) Liposomal ophthalmic drug delivery system. Dihydrostreptomycin sulfate. Int J Pharm 19(3):263–269CrossRefGoogle Scholar
  19. Suleymanoglu E (2004) A nanoscale polynucleotide-neutral liposome self-assemblies formulated. Rev Electron Biomed 2:13–25Google Scholar
  20. Wu G (2009) Chap. 14 Synthesis, Characterization, and Optical Response of Gold Nanoshells Used to Trigger Release from Liposomes. In: Methods in Enzymology. D. Nejat, Editor; 279 – 89Google Scholar
  21. Zasadzinski JA (2011) Novel methods of enhanced retention in and rapid, targeted release from liposomes. Curr Opin Colloid Interface Sci 16(3):203–214CrossRefGoogle Scholar

Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.Shahid Bahonar University of KermanKermanIran
  2. 2.Tehran UniversityTehranIran

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