Preparation of antibacterial peel-off facial mask formulation incorporating biosynthesized silver nanoparticles

  • Amruta U. Badnore
  • Karuna I. Sorde
  • Kirti A. Datir
  • Laxmi Ananthanarayan
  • Amit P. Pratap
  • Aniruddha B. PanditEmail author
Original Article


The micro-organism-mediated nanoparticle synthesis is of great importance in the nano-field due to its economic viability, higher and easy bioaccumulation and the process can be scaled up relatively easily. The downstream processing and biomass handling are also facilitated. It is also known to occur naturally. In the current study, the biological synthesis of silver nanoparticles (AgNPs) using Bacillus nakamurai sp. (isolated from soil) is reported. The as-synthesized nanoparticles were characterized using X-ray diffraction to confirm AgNP formation. On characterizing the biosynthesized AgNPs with different analyzing tools, the spherical shape of particles with hydrodynamic size 379.2 nm was observed. To the best of our knowledge, this is the first report to establish an antibacterial peel-off facial mask formulation employing biosynthesized AgNPs. The current study has demonstrated the antibacterial activity of the prepared peel-off facial mask formulation against Pseudomonas aeruginosa, Staphylococcus aureus and Propionibacterium acnes. The antibacterial formulation exhibited the lowest susceptibility to all three bacterial strains and the zone of inhibition for the formulations ranged from 8\(\hspace{0.17em}\hspace{0.17em}\pm \hspace{0.17em} 0.1\) to 14 \(\pm \hspace{0.17em} 0.1 \text{mm}\). Besides, in the case of control formulation, no resistance to the bacterial strains was revealed. Furthermore, the cost estimation of the prepared antibacterial formulation has also been assessed.


AgNPs Nakamurai sp. Antibacterial formulation Regeneration 



Financial support from University Grants Commission (UGC), India, is gratefully acknowledged. Authors AUB and KIS thank UGC for their research fellowship.

Compliance with ethical standards

Conflict of interest

On behalf of all authors, the corresponding author states that there is no conflict of interest.


  1. Agnihotri S, Mukherji S, Mukherji S (2012) Antimicrobial chitosan-PVA hydrogel as a nanoreactor and immobilizing matrix for silver nanoparticles. Appl Nanosci 2:179–188CrossRefGoogle Scholar
  2. Ahmed R, Minaeian S, Shahverdi HR, Jamalifar H, Nohi A (2007) Rapid synthesis of silver nanoparticles using culture supernatants of enterobacteria: a novel approach. Process Biochem 42:919–923CrossRefGoogle Scholar
  3. Badnore U, Pandit AB (2017) Effect of pH on sonication assisted synthesis of ZnO nanostructures: process details. Chem Eng Process 122:235–244CrossRefGoogle Scholar
  4. Beyene HD, Werkneh AA, Bezabh HK, Ambaye TG (2017) Synthesis paradigm and applications of silver nanoparticles (AgNPs), a review. Sustain Mater Technol 13:18–23Google Scholar
  5. Dakal TC, Kumar A, Majumdar RS, Yadav V (2016) Mechanistic basis of antimicrobial actions of silver nanoparticles. Front Microbiol 7:1831CrossRefGoogle Scholar
  6. Dehghanizade S, Arasteh J, Mirzaie A (2018) Green synthesis of silver nanoparticles using anthemis atropatana extract: characterization and in vitro biological activities. Artif Cell Nanomed Biotechnol 46:160–168CrossRefGoogle Scholar
  7. Dhand C, Dwivedi N, Loh XJ, Ying AN, Verma NK, Beuerman RW, Lakshminarayanan R, Ramakrishna S (2015) Methods and strategies for the synthesis of diverse nanoparticles and their applications: a comprehensive overview. RSC Adv 5:105003–105037CrossRefGoogle Scholar
  8. Fouad H, Hongjie L, Yanmei D, Baoting Y, Shakh AE, Abbas G, Jianchu M (2017) Synthesis and characterization of silver nanoparticles using Bacillus nakamurai and Bacillus subtilis to control filarial vector culex pipiens pallens and its antimicrobial activity. Artif Cell Nanomed Biotechnol 45:1369–1378CrossRefGoogle Scholar
  9. Fulekar MH (2010) Nanotechnology: importance and application. IK International publishing house, New DelhiGoogle Scholar
  10. Kokura S, Handa O, Takagi T, Ishikawa T, Naito Y, Yoshikawa T (2010) Silver nanoparticles as a safe preservative for use in cosmetics. Nanomed Nanotechnol 4:570–574CrossRefGoogle Scholar
  11. Lin Y, Yan L (2004) Broad spectrum anti-bactericidal ointment nano. CN Patent. CN. 1480045A, App No: CN 03130426Google Scholar
  12. Mukherji S, Ruparelia J, Agnihotri S (2012) Nano-antimicrobials progress and prospects. In: Cioffi N, Rai M (eds) Antimicrobial activity of silver and copper nanoparticles: variation in sensitivity across various strains of bacteria and fungi. Springer, Germany, pp 226–251Google Scholar
  13. Munoz RV, Borrego B, Moreno KJ, Garcia MG, Morales JDM, Bogdanchikova N, Saquero AH (2017) Toxicity of silver nanoparticles in biological systems: does the complexity of biological systems matter? Toxicol Lett 276:11–20CrossRefGoogle Scholar
  14. Noronha VT, Paula AJ, Duran G, Galembeck A, Muller KC, Montan MF, Duran N (2017) Silver nanoparticles in dentistry. Dent Mater 33:1110–1126CrossRefGoogle Scholar
  15. Raj S, Jose S, Sumod US, Sabitha M (2012) Nanotechnology in cosmetics: opportunities and challenges. J Pharm Bioall Sci 4:186–193CrossRefGoogle Scholar
  16. Singh P, Kim YJ, Zhang D, Yang DC (2016) Biological synthesis of nanoparticles from plants and microorganisms. Trends Biotechnol 34:588–599CrossRefGoogle Scholar
  17. Singla R, Guliani A, Kumari A, Yadav SK (2016) Metallic nanoparticles, toxicity issues and applications in medicine. In: Yadav S (ed) Nanoscale materials in targeted drug delivery, Theragnosis and tissue regeneration. Springer, SingaporeGoogle Scholar
  18. Waterhouse GIN, Bowmaker GA, Metson JB (2001) The thermal decomposition of silver (I, III) oxide: a combined XRD, FT-IR and Raman spectroscopic study. Phys Chem Chem Phys 3:3838–3845CrossRefGoogle Scholar
  19. Zhang XF, Liu ZG, Shen W, Gurunathan S (2016) Silver nanoparticle: synthesis, characterization, properties, applications, and therapeutic approaches. Int J Mol sci 17:1534CrossRefGoogle Scholar

Copyright information

© King Abdulaziz City for Science and Technology 2018

Authors and Affiliations

  • Amruta U. Badnore
    • 1
  • Karuna I. Sorde
    • 2
  • Kirti A. Datir
    • 3
  • Laxmi Ananthanarayan
    • 2
  • Amit P. Pratap
    • 3
  • Aniruddha B. Pandit
    • 1
    Email author
  1. 1.Department of Chemical EngineeringInstitute of Chemical TechnologyMumbaiIndia
  2. 2.Department of Food Engineering and TechnologyInstitute of Chemical TechnologyMumbaiIndia
  3. 3.Department of Oils, Oleochemicals and Surfactants TechnologyInstitute of Chemical TechnologyMumbaiIndia

Personalised recommendations