Applied Nanoscience

, Volume 9, Issue 1, pp 43–48 | Cite as

Incorporation of magnetic nanoparticle to graphene oxide via simple emulsion method and their cytotoxicity

  • Emmellie Laura Albert
  • Muhammad Bilal Sajiman
  • Che Azurahanim Che AbdullahEmail author
Original Article


Magnetite nanoparticle and graphene oxide is a promising nanoparticle that can be used in multitude of field due to their exceptional characteristic. Graphene oxide has a unique 2-D structure, and excellent chemical and physical characteristics while magnetite nanoparticle has its superparamagnetic properties which enable it to be controlled by external magnetic field. Owing to that, any new formulations of magnetic nanoparticle functionalities with graphene oxide have to be taken into consideration. In this research, magnetite nanoparticles were functionalized with graphene oxide using simple emulsion and evaporation method. All the samples were characterized by X-ray diffraction, and Fourier-transform infrared, and Raman spectroscopy. The toxicity of the nanomaterials was tested with cell viability assay (XTT) using A549 cells. The cell viability remains high within 24 h and 72 h of incubation, and when the concentration increases up to 100 µg/mL only a slight decrease of viability was observed.


Magnetic nanoparticle Graphene oxide Emulsion technique 



Emmellie Laura Albert and Muhammad Bilal Sajiman have contributed equally to this project with the supervision and guidance of Che Azurahanim Che Abdullah. We would like to express our deepest gratitude to the NANOTEDD members in the Biophysics Laboratory and supporting staff of Department of Physics, UPM. The part of this work was supported by the FRGS Grant provided by Ministry of Higher Education (FRG S5524949) and Dana Tautan UPM (DT0021) financed by Universiti Putra Malaysia.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.


  1. Agarwal S, Zhou X, Ye F, He Q, Chen GC, Soo J, Boey F, Zhang H, Chen P (2010) Interfacing live cells with nanocarbon substrates. Langmuir 26(4):2244–2247CrossRefGoogle Scholar
  2. Ahangaran F, Hassanzadeh A, Nouri S (2013) Surface modification of Fe3O4@SiO2 microsphere by silane coupling agent. Int Nano Lett 3(1):23. CrossRefGoogle Scholar
  3. Aliabadi M, Shagholani H, Yunessnia lehi A (2017) Synthesis of a novel biocompatible nanocomposite of graphene oxide and magnetic nanoparticles for drug delivery. Int J Biol Macromol 98:287–291. CrossRefGoogle Scholar
  4. Ang PK, Chen W, Wee ATS, Loh KP (2008) Solution-gated epitaxial graphene as pH sensor. J Am Chem Soc 130(44):14392–14393CrossRefGoogle Scholar
  5. Balandin AA, Ghosh S, Bao W, Calizo I, Teweldebrhan D, Miao F, Lau CN (2008) Superior thermal conductivity of single-layer graphene. Nano Lett 8(3):902–907CrossRefGoogle Scholar
  6. Becerril HA, Mao J, Liu Z, Stoltenberg RM, Bao Z, Chen Y (2008) Evaluation of solution-processed reduced graphene oxide films as transparent conductors. ACS Nano 2(3):463–470CrossRefGoogle Scholar
  7. Booth TJ, Blake P, Nair RR, Jiang D, Hill EW, Bangert U, Bleloch A, Gass M, Novoselov KS, Katsnelson MI (2008) Macroscopic graphene membranes and their extraordinary stiffness. Nano Lett 8(8):2442–2446CrossRefGoogle Scholar
  8. Bordbar AK, Rastegari AA, Amiri R, Ranjbakhsh E, Abbasi M, Khosropour AR (2014) Characterization of modified magnetite nanoparticles for albumin immobilization. Biotechnol Res Int. Google Scholar
  9. Chang Y, Yang ST, Liu JH, Dong E, Wang Y, Cao A, Liu Y, Wang H (2011) In vitro toxicity evaluation of graphene oxide on A549 cells. Toxicol Lett 200(3):201–210. CrossRefGoogle Scholar
  10. Das A, Pisana S, Chakraborty B, Piscanec S, Saha S, Waghmare U, Novoselov K, Krishnamurthy H, Geim A, Ferrari A (2008) Monitoring dopants by Raman scattering in an electrochemically top-gated graphene transistor. Nat Nanotechnol 3(4):nnano.2008.2067CrossRefGoogle Scholar
  11. Fakruddin M, Hossain Z, Afroz H (2012) Prospects and applications of nanobiotechnology: a medical perspective. J Nanobiotechnol 10(1):31CrossRefGoogle Scholar
  12. He F, Fan J, Ma D, Zhang L, Leung C, Chan HL (2010) The attachment of Fe3O4 nanoparticles to graphene oxide by covalent bonding. Carbon. Google Scholar
  13. Hu W, Peng C, Luo W, Lv M, Li X, Li D, Huang Q, Fan C (2010) Graphene-based antibacterial paper. ACS Nano 4(7):4317–4323CrossRefGoogle Scholar
  14. Kassaee MZ, Motamedi E, Majdi M (2011a) Magnetic Fe3O4-graphene oxide/polystyrene: fabrication and characterization of a promising nanocomposite. Chem Eng J 172(1):540–549. CrossRefGoogle Scholar
  15. Kassaee MZ, Motamedi E, Majdi M (2011b) Magnetic Fe3O4-graphene oxide/polystyrene: fabrication and characterization of a promising nanocomposite. Chem Eng J. Google Scholar
  16. Kumar ASK, Rajesh N (2013) Exploring the interesting interaction between graphene oxide, Aliquat-336 (a room temperature ionic liquid) and chromium(vi) for wastewater treatment. RSC Adv 3(8):2697–2709. CrossRefGoogle Scholar
  17. Kumar N, Das S, Bernhard C, Varma GD (2013) Effect of graphene oxide doping on superconducting properties of bulk MgB2. Supercond Sci Technol. Google Scholar
  18. Laurent S, Forge D, Port M, Roch A, Robic C, Vander Elst L, Muller RN (2008) Magnetic iron oxide nanoparticles: synthesis, stabilization, vectorization, physicochemical characterizations, and biological applications. Chem Rev 108(6):2064–2110CrossRefGoogle Scholar
  19. Lee C, Wei X, Kysar JW, Hone J (2008) Measurement of the elastic properties and intrinsic strength of monolayer graphene. Science 321(5887):385–388CrossRefGoogle Scholar
  20. Lu AH, Salabas EeL, Schüth F (2007) Magnetic nanoparticles: synthesis, protection, functionalization, and application. Angew Chem Int Ed 46(8):1222–1244CrossRefGoogle Scholar
  21. Maeda Y, Yoshino T, Matsunaga T (2009) Novel nanocomposites consisting of in vivo-biotinylated bacterial magnetic particles and quantum dots for magnetic separation and fluorescent labeling of cancer cells. J Mater Chem 19(35):6361–6366CrossRefGoogle Scholar
  22. Marcano DC, Kosynkin DV, Berlin JM, Sinitskii A, Sun Z, Slesarev A, Alemany LB, Lu W, Tour JM (2010) Improved synthesis of graphene oxide. ACS Nano 4(8):4806–4814. CrossRefGoogle Scholar
  23. Muszynski R, Seger B, Kamat PV (2008) Decorating graphene sheets with gold nanoparticles. J Phys Chem C 112(14):5263–5266CrossRefGoogle Scholar
  24. Novoselov KS, Geim AK, Morozov SV, Jiang D, Zhang Y, Dubonos SV, Grigorieva IV, Firsov AA (2004) Electric field effect in atomically thin carbon films. Science 306(5696):666–669CrossRefGoogle Scholar
  25. Paulchamy B, Arthi G, Lignesh B (2015) A simple approach to stepwise synthesis of graphene oxide nanomaterial. J Nanomed Nanotechnol 6(1):1Google Scholar
  26. Peng T, Sun H, Peng T, Liu B, Zhao X (2017) Structural regulation and electroconductivity change of nitrogen-doping reduced graphene oxide prepared using p-phenylene diamine as modifier. Nanomaterials 7(10):292CrossRefGoogle Scholar
  27. Schedin F, Geim A, Morozov S, Hill E, Blake P, Katsnelson M, Novoselov K (2007) Detection of individual gas molecules adsorbed on graphene. Nat Mater 6(9):652CrossRefGoogle Scholar
  28. Shen J, Hu Y, Shi M, Li N, Ma H, Ye M (2010) One step synthesis of graphene oxide—magnetic nanoparticle composite. J Phys Chem C 114(3):1498–1503CrossRefGoogle Scholar
  29. Stoller MD, Park S, Zhu Y, An J, Ruoff RS (2008) Graphene-based ultracapacitors. Nano Lett 8(10):3498–3502CrossRefGoogle Scholar
  30. Wang K, Ruan J, Song H, Zhang J, Wo Y, Guo S, Cui D (2010) Biocompatibility of graphene oxide. Nanoscale Res Lett 6(1):8. Google Scholar
  31. Watanabe M, Yoneda M, Morohashi A, Hori Y, Okamoto D, Sato A, Kurioka D, Nittami T, Hirokawa Y, Shiraishi T, Kawai K, Kasai H, Totsuka Y (2013) Effects of Fe(3)O(4) magnetic nanoparticles on A549 Cells. Int J Mol Sci 14(8):15546–15560. CrossRefGoogle Scholar
  32. Wu X, Liu P (2010) Facile preparation and characterization of graphene nanosheets/polystyrene composites. Macromol Res 18(10):1008–1012CrossRefGoogle Scholar
  33. Yang X, Zhang X, Ma Y, Huang Y, Wang Y, Chen Y (2009) Superparamagnetic graphene oxide–Fe3O4 nanoparticles hybrid for controlled targeted drug carriers. J Mater Chem 19(18):2710–2714CrossRefGoogle Scholar
  34. Yoo E, Kim J, Hosono E, Zhou H-s, Kudo T, Honma I (2008) Large reversible Li storage of graphene nanosheet families for use in rechargeable lithium ion batteries. Nano Lett 8(8):2277–2282CrossRefGoogle Scholar
  35. Zhang W, Cui J, Tao CA, Wu Y, Li Z, Ma L, Wen Y, Li G (2009) A strategy for producing pure single-layer graphene sheets based on a confined self-assembly approach. Angew Chem Int Ed 48(32):5864–5868. CrossRefGoogle Scholar
  36. Zhang M, Jia M, Jin Y (2012) Fe3O4/reduced graphene oxide nanocomposite as high performance anode for lithium ion batteries. Appl Surf Sci 261(Supplement C):298–305. CrossRefGoogle Scholar

Copyright information

© King Abdulaziz City for Science and Technology 2018

Authors and Affiliations

  1. 1.Department of Physics, Faculty of ScienceUniversity Putra MalaysiaSerdangMalaysia
  2. 2.Materials Synthesis and Characterization Laboratory, Institute of Advance TechnologyUniversity Putra MalaysiaSerdangMalaysia
  3. 3.Biophysics Lab, Integrated Chemical Biophysics, Faculty of ScienceUniversity Putra MalaysiaSerdangMalaysia

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