Preparation of carboxy-methyl cellulose-capped nanosilver particles and their antimicrobial evaluation by an automated device
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Colloidal solution of nano silver particles (AgNPs) have been prepared using carboxymethyl cellulose as the stabilizing agent and dextrose as the reducing agent. It is considered bio-friendly, as all ingredients at much higher concentrations are used on eye as medicine. AgNPs thus generated are triangular with 9.5 nm size. MIC values of AgNPs and equivalent ionic silver against different multi-drug resistant strain bacteria and yeast are determined by microdilution method. Biological parameters for nano conversion are indicated by 128–256-fold higher sensitivity. Selective range synergisms for 1/4th MIC AgNPs with battery of antimicrobial agents are indicated by automated susceptibility testing device, keeping a negative control. Much lower MICs for different resistant antibiotics in combination with AgNPs are noted for all test organisms. This indicates scope for using a tolerable concentration of nanoantimicrobials either alone or in combination with an empirically chosen antibiotic for managing surface infections of eye or skin.
KeywordsSilver nanoparticles Carboxy-methyl cellulose Synergism Topical anti-microbial Automated susceptibility testing
PKM conceived the project and designed the experiments dealing with synthesis, storage of eye tolerable AgNPs and new methods for determining anti-microbial properties. AG and RP carried out experiments dealing with synthesis, characterizations and microbiological experiments with AgNPs. MRC, AG and AS carried out the electron microscopic studies and experiments related to physical characterization of AgNPs. All authors were involved with interpretation of obtained results, preparation and editing the manuscript.
Compliance with ethical standards
Conflict of interest
Applied for Patent in India by Prasanta Kumar Maiti; Application no. 201831009290, Date of publication 6th, April, 2018.
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