Applied Nanoscience

, Volume 9, Issue 1, pp 49–66 | Cite as

Accumulation of lead (Pb II) metal ions by Bacillus toyonensis SCE1 species, innate to industrial-area ground water and nanoparticle synthesis

  • Blessy Baby MathewEmail author
  • Vinai George Biju
  • Krishnamurthy Nideghatta Beeregowda
Original Article


Groundwater samples were collected from Peenya Industrial area of Bengaluru, India to check its inherent bacterial population. After the isolates were grown in lead-induced media, the bacteria that could resist lead were further isolated, identified, and grown. The isolated bacterium was identified as Bacillus toyonensis SCE1 species, and the maximum amount of lead taken up by it to sustain itself as a live biomass was 8 ppm. Lead accumulation was studied based on different factors such as pH, concentration, and time. Bacterial characterization through scanning electron microscopy showed there was lead sorption on the bacterial cells. Transmission electron microscopy revealed that accumulated lead ions inside the bacteria were in the range of 180–190 nm, this was found to correlate with the data obtained by the particle size analyzer. The optimum pH for highest lead accumulation was 7.2, for a time period of 160 min. It was observed that the stable production of homogenous nanoparticles using biomass could be achieved by optimizing factors such as incubation period, agitation, pH, temperature, and contact time.


Bioaccumulation Lead toxicity Bacillus toyonensis Industrial ground water 



The authors thank Dr. Sudhir H Ranganath (Department of Chemical Engineering, Siddaganga Institute of Technology, Tumkur) for his expertise in Dynamic Light Scattering technique, Dr. DS Prasanna and Mr. Murthy (Dept. of Nanotechnology, VTU, Chikkabalapura) for FTIR analysis, Mr. John (TBI, Vellore Institute of Technology, Vellore) and TUV SUD, Bengaluru for AAS and ICPOES analysis, Dr. Chandan Srivastava (Department of Material Science, IISc, Bengaluru) for his expertise in Scanning Electron Microscopy and Energy Dispersive X-ray analysis and Mr. Adarsh (STIC, Cochin University of Science and Technology, Cochin) for his assistance with HR-TEM technique. The authors also wish to acknowledge the lab facilities provided by the Department of Biotechnology, Sapthagiri College of Engineering (SCE), Bangalore and the Principal and Management of SCE for their support.

Conflict of interest

On behalf of all authors, the corresponding author states that there is no conflict of interest.


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© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of BiotechnologySapthagiri College of EngineeringBengaluruIndia
  2. 2.Department of Computer Science and EngineeringChrist UniversityBengaluruIndia

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