Alleviation of salinity-induced damage on wheat plant by an ACC deaminase-producing halophilic bacterium Serratia sp. SL- 12 isolated from a salt lake
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Plant growth promoting bacteria (PGPB) with 1-aminocyclopropane-1-carboxylate deaminase (ACC deaminase) activity can be used to ameliorate salt stress in plants. The aim of this study was to characterize a salt-tolerant PGP bacterium Serratia sp. SL-12 isolated from a salt lake, and to evaluate its capability to promote growth in wheat plants (Triticum aestivum L) under conditions of salt stress. The isolate SL-12 exhibited other plant growth promoting properties such as the production of indole-3-acetic acid, and enabling solubilization of inorganic phosphate. An analysis of fatty acid composition of the isolate grown at different salt concentrations (150–200 mM) indicated that salt concentration strongly influenced the fatty acid composition, and increased the proportion of unsaturated fatty acids. Inoculation of SL-12 into wheat plants growing under salt stress (150–200 mM NaCl) resulted in a significant increase in plant growth, as measured by parameters such as shoot/root length, fresh/dry weight, and photosynthetic pigment accumulation. In addition, application of isolate SL-12 decreased the levels of Na+ by (65 %) and increased the uptake of K+ by (39 %), indicating a role in maintaining ionic homeostasis, and minimizing toxic ionic effects in host wheat plants. The growth of wheat seedling under salinity stress was improved by SL-12 by inducing accumulation of osmolytes such as total soluble sugar and total protein content, while reducing the salt-induced malondialdehyde content. This has been found by other researchers. The present study indicates the potential of isolate SL-12 as a biofertilizer for enhancing the growth of wheat and other crops under salt stress conditions.
KeywordsACC deaminase GC-MS Fatty acids Osmolytes Salt stress Wheat
This research was financially supported by supported by Department of Biotechnology (File No. BT/PR14527/AGR/21/326/2010), Government of India, New Delhi to PNJ. The authors are thankful to Mr. Manoj Kannan, Lecturer, Biological Sciences Department, BITS Pilani for providing editorial and language assistance..
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Conflict of interest
There is no conflict of interest in this work.
- Ahmad F, Ahmad I, Khan MS (2008) Screening of free-living rhizospheric bacteria for their multiple growth promoting activities. Microbiol 163:173–181Google Scholar
- Cappuccino JG, Sherman N (1992) Biochemical activities of microorganisms In: Microbiology, A Laboratory Manual. The Benjamin / Cummings Publishing Co California, USAGoogle Scholar
- Christie WW (1993) Preparation of ester derivatives of fatty acids for chromatographic analysis. Adv Lipid Methodol 2:69–111Google Scholar
- Dobereiner J (1995) Isolation and identification of aerobic nitrogen-fixing bacteria from soil and plants. In: Alef K, Nannipieri P (eds) Methods in applied soil microbiology and biochemistry. Academic, London, pp 134–141Google Scholar
- El-Tarabily KA (2008) Promotion of tomato (Lycopersicon esculentum Mill.) plant growth by rhizosphere competent 1-aminocyclopropane-1-carboxylic acid deaminase-producing streptomycete actinomycetes. Plant Soil 308:161–174Google Scholar
- George P, Gupta A, Murali GM, Thomas L, Thomas GV (2012) Multifarious beneficial traits and plant growth promoting potential of Serratia marcescens KiSII and Enterobacter sp RNF 267 isolated from the rhizosphere of coconut palms (Cocos nucifera L). World J Microbiol Biotechnol 29:109–117CrossRefPubMedGoogle Scholar
- Han HS and Lee KD (2005) Plant growth promoting rhizobacteria effect on antioxidant status, photosynthesis, mineral uptake and growth of lettuce under soil salinity. Res J Agr Biological Sci 1:210–215Google Scholar
- Harley JP, Prescott LM (2002) Laboratory exercises in microbiology, 5th edn. McGraw-Hill Companies, TexasGoogle Scholar
- Khan MA, Shirazi MU, Muhammad AK, Mujtaba SM, Islam E, Mumtaz S, Shereen A, Ansari RU, Afhraf Y (2009) Role of proline, K/Na ratio and chlorophyll content in salt tolerance of wheat (Triticum aestivum L.). Pak J Bot 41:633–638Google Scholar
- Kumar RS, Ayyadurai N, Pandiaraja P, Reddy AV, Venkateswarlu Y, Prakash O, Sakthivel N (2005) Characterization of antifungal metabolite produced by a new strain Pseudomonas aeruginosa PUPa3 that exhibits broadspectrum antifungal activity and biofertilizing traits. J Appl Microbiol 98:145–154Google Scholar
- Marinetti GV (1962) Chromatographic separation: identification and analysis of phospholipids. J Lipid Res 3:1–20Google Scholar
- Mehta S, Nautiyal CS (2001) An efficient method for qualitative screening of Phosphate-solubilizing bacteria. Microbiol 43:51–56Google Scholar
- Pathak PS (2000) Agroforestry: a tool for arresting land degradation. Indian Farming 49:15–19Google Scholar
- Sarkar S, Sreekanth B, Kant S, Banerjee R, Bhattacharyya BC (1998) Production and optimization of microbial lipase. Bioprocess Eng 19:29–32Google Scholar
- Wang XL (2008) Study on liquid fermentation technology of Bacillus B579 and its living preparation Dissertation, Tianjin University of Science and Technology (in Chinese)Google Scholar
- Yao LX, Wu ZS, Zheng YY, Kaleem I, Li C (2010) Growth promotion and protection against salt stress by Pseudomonas putida Rs-198 on cotton. Eur J Soil Biol 46:49–54Google Scholar