Genotypic variation of nodules’ enzymatic activities in symbiotic nitrogen fixation among common bean (Phaseolus vulgaris L.) genotypes grown under salinity constraint
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The effect of salt stress, under glasshouse conditions, was studied on plant biomass, nodulation, and activities of acid phosphatases (APase, EC 184.108.40.206) and trehalose 6-phosphate phosphatase (TPP, EC 220.127.116.11) in the symbiosis common bean (Phaseolus vulgaris L.)-rhizobia nodules. Four common bean recombinant inbred lines (147, 115, 104 and 83) were separately inoculated, with CIAT 899 or RhM11 strains and grown in hydroaeroponic culture. Two NaCl levels (0 and 25 mM NaCl plant−1 week−1 corresponding, respectively, to the control and the salt treatment) were applied and the culture was assessed during 42 days after their transplantation. The results showed that the nodulation of these lines was not affected by salinity except for the line 83 inoculated with CIAT 899, whose nodule dry weight decreased by 48.24 % compared with the corresponding controls. For the other symbiotic combinations, shoot and root biomasses were not significantly affected by salt constraint. Salinity stress generally reduced acid phosphatise and trehalose phosphate phosphatase activities in nodules that were less affected in plants inoculated with RhM11. Based on our data, it appears that nodule phosphatase activity may be involved in salinity tolerance in common beans and the levels of salt tolerance depend principally on specific combination of the rhizobial strain and the host cultivar.
KeywordsCommon bean Nodulation Salinity constraint Acid phosphatase Trehalose phosphate phosphatase RT-PCR in situ
This work was financially supported by PRAD project N° 06-08. The authors wish to thank Hélène Vailhe (INRA, Montpellier) for her technical assistance.
- Esfandiari E, Shekari F, Shekari F, Esfandiari M (2007) The effect of salt stress on antioxidant enzymes activity and lipid peroxidation on the wheat seedling. Not Bot Hort Agrobot Cluj 35:48–56Google Scholar
- Faghire M, Bargaz A, Farissi M, Palma F, Mandri B, Lluch C, Tejera García NA, Herrera-Cervera JA, Oufdou K, Ghoulam C (2011) Effect of salinity on nodulation, nitrogen fixation and growth of common bean (Phaseolus vulgaris) inoculated with rhizobial strains isolated from the Haouz region of Morocco. Symbiosis 55:69–75CrossRefGoogle Scholar
- Georgiev GI, Atkins CA (1993) Effects of salinity on N2 fixation, nitrogen metabolism and export and diffusive conductance of cowpea root nodules. Symbiosis 15:239–255Google Scholar
- Jaleel CA, Gopi R, Manivannan P, Panneerselvam R (2007) Antioxidative potentials as a protective mechanism in Catharanthusroseus (L.) G. Don. Plants under salinity stress. Turk J Bot 31:245–251Google Scholar
- Lauchli A (1984) Salt exclusion: an adaptation of legume for crops and pastures under saline conditions. In: Staples RC, Toenniessen GH (eds) Salinity tolerance in plants. Strategies for crop improvement. John Wiley and Sons, New York, pp 171–187Google Scholar
- Lopez M, Lluch C (2012) Trehalose and abiotic stress tolerance. Abiotic stress responses in plants. 253–265. doi:10.1007/978-1-4614-0634-1_14
- Mandri B, Drevon JJ, Bargaz A, Oufdou K, Faghire M, Plassard C, Payre H, Ghoulam C (2012) Interactions between common bean genotypes and rhizobia strains isolated from moroccan soils for growth, phosphatase and phytase activities under phosphorus deficiency conditions. J Plant Nutr 35:1477–1490CrossRefGoogle Scholar
- Olmos E, Hellin E (1997) Cytochemical localization of ATPase plasma membrane and acid phosphatase by cerium based in a salt-adapted cell line of Pisumsativum. J Exp Bot 48:1529–1535Google Scholar
- Sairam RK, Tyagi A (2004) Physiological and molecular biology of salinity stress tolerance in plants. Curr Sci 86:407–420Google Scholar
- Soussi M, Ocana A, Lluch C (1998) Effect of salt stress on growth, photosynthesis and nitrogen fixation in chickpea (CicerarietinumL.). J Exp Bot 325:1329–1337Google Scholar
- Soussi M, Lluch C, Ocana A (1999) Comparative study of nitrogen fixation and carbon metabolism in two chick-pea (Cicerarietinum L.) cultivars under salt stress. J Exp Bot 50:1701–1708Google Scholar