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Symbiosis

, Volume 56, Issue 2, pp 87–95 | Cite as

Monoxenic nodulation process of Acacia mangium (Mimosoideae, Phyllodineae) by Bradyrhizobium sp

  • Marie-Mathilde Perrineau
  • Antoine Galiana
  • Sergio M. de Faria
  • Gilles Bena
  • Robin Duponnois
  • Paul Reddell
  • Yves PrinEmail author
Article

Abstract

Acacia mangium Willd., a native tree in Australia and Papua New Guinea, has been introduced to countries in Asia and South America where plantations have been established that cover several hundred thousand ha. The present study investigated the early stages of the nodulation process in A. mangium using an homologous Australian Bradyrhizobium strain. After optimizing the axenic nodulation, histological and cytological studies were conducted using light and electron microscopy. These documented the proliferation of Bradyrhizobium, the lysis of mucilage at the root surface, root hair deformation and initiation, as well as the development and growth of multiple infection threads. A belt of tannin-filled cells was shown to surround the central nodular fixation zone. The nodules were of the indeterminate type and the bacteroids had a rod shape, without size modification and with few polyhydroxybutyrate (PHB) granules. Several bacteroids can share the same symbiosome. A. mangium exhibits both classical and novel features in its nodulation.

Keywords

Nitrogen fixation Root hair Infection threads Indeterminate nodule 

Notes

Acknowledgements

This work was supported by Centre de coopération Internationale pour la Recherche Agronomique et le Développement (CIRAD) (PhD grant to M.M.P.). This work was partially funded by the French Ministère de l’Environnement et du Développement Durable (Programme Ecofor/Ecosystèmes Tropicaux).

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Marie-Mathilde Perrineau
    • 1
  • Antoine Galiana
    • 1
  • Sergio M. de Faria
    • 2
  • Gilles Bena
    • 3
  • Robin Duponnois
    • 3
  • Paul Reddell
    • 4
  • Yves Prin
    • 1
    • 5
    Email author
  1. 1.CIRAD, UMR LSTMMontpellier Cedex 5France
  2. 2.EMBRAPASeropedicaBrazil
  3. 3.IRD, UMR LSTMMontpellier Cedex 5France
  4. 4.Ecobiotics SAYungaburraAustralia
  5. 5.LSTM, TA A-82/JMontpellier Cedex 5France

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