Chromosome numbers are not fixed in Agaonidae (Hymenoptera: Chalcidoidea)
Agaonidae (the pollinators of fig trees, Ficus spp., Moraceae) are a distinctive family of chalcid wasps with uncertain affinities within the Chalcidoidea. Chromosome numbers have only been described previously for one species (Blastophaga psenes, 2n = 12, the pollinator of F. carica, of subgenus Ficus). In this paper, we used a modified technique to analyze the karyology of three Ceratosolen species, Ceratosolen solmsi, C. graveli and C. emarginatus which are associated with figs of Ficus subgenus Sycomorus. Their karyotypes are 2n = 10. All the chromosomes are metacentric (NF = 20) with little difference in relative lengths, making them hard to distinguish. The variation in chromosome numbers that we have detected suggests that karyology can usefully complement molecular-based studies of the phylogeny of fig wasps.
KeywordsCeratosolen Chromosomes Fig wasps Karyology
This work was funded by the Chinese Natural Science Foundation (30970403, 30970439) and also supported by grants from the Key Laboratory of Forest Disaster Warning and Control Program of Southwest Forestry University (ZK10B302). We express our sincere gratitude to V.E. Gokhman (Botanical Garden, Moscow State University) and Wen-hui Nie (Kunming Institute of Zoology, Chinese Academy of Science) for their good suggestions on the experiment and Rodrigo A. S. Pereira for commenting on an earlier version of the manuscript. We thank Sun Fuping for his help on image processing. We also thank Cao Yong-qiang, Yang Pei, Li Zong-bo, Ma Wen-juan, Xi Yi-cui, Miao Bai-ge and Wang Gang for their assistance.
- Berg CC, Corner EJH (2005) Moraceae-Ficus. Flora Malesiana, Ser, I, 17⁄2, LeidenGoogle Scholar
- Bouček Z (1988) Australasian Chalcidoidea (Hymenoptera): a biosystematic revision of genera of fourteen families, with a reclassification of species. CAB International, WallingfordGoogle Scholar
- Campbell B, Heraty J, Rasplus JY, Cha K, Steffen-Campbell J, Babcock C (2000) Molecular systematics of the Chalcidoidea using 28S-D2 rDNA. In: Austin AD, Dowton M (eds) Hymenoptera: evolution, biodiversity and biological control. CSIRO, Collingwood, 59ppGoogle Scholar
- Gokhman VE (2009) Karyotypes of parasitic Hymenoptera. Springer Science + Business Media B.V., Dordrecht. XIIIGoogle Scholar
- Gokhman VE, Quicke DLJ (1995) The last twenty years of parasitic Hymenoptera karyology: an update and phylogenetic implications. J Hymenopt Res 4:41–63Google Scholar
- Gokhman VE, Mikhailenko AP, Fursov VN (2010) Chromosomes of Blastophaga psenes (Hymenoptera: Agaonidae). J Hymenopt Res 19:187–188Google Scholar
- Ling FY (1984) Studies on the karyotypes of seven species of flies. Zool Res (Suppl.) 5:51–56 (In Chinese with English abstract)Google Scholar
- Ramirez WB (1978) Evolution of mechanisms to carry pollen in Agaonidae (Hymenoptera, Chalcidoidea). Tijdschr Entomol 121:279–293Google Scholar
- Wiebes JT (1963) Taxonomy and host preferences of Indo-Australian fig wasps of the genus Ceratosolen (Agaonidae). Tijdschr Entomol 106:1–112Google Scholar
- Wiebes JT (1994) The Indo-Australian Agaoninae (pollinators of figs). North-Holland, Amsterdam, p 208Google Scholar
- Zhang FP, Peng YQ, Guan JM, Yang DR (2008) A species of fig tree and three unrelated fig wasp pollinators. Evol Ecol Res 10:611–620Google Scholar