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Synergistic effects of ultrasound and extraction solvent on the bioactive compound in kenaf seed oil

  • Zhen-shan ZhangEmail author
  • Qing-fang Xie
  • Liming CheEmail author
Original Article
  • 33 Downloads

Abstract

Kenaf seed oil was extracted with 3 different solvents, i.e. hexane, ethanol and aqueous enzymatic medium with or without ultrasonic assistance. The synergistic effects of ultrasound and extraction solvent on the content of bioactive compound in kenaf seed oil was investigated. Results show that ultrasound-assisted extraction with hexane obtained the highest yield (84.71%), while yield with aqueous enzymatic medium was the lowest (51.12%). Two endothermic peaks exhibited on the melting curve of kenaf seed oil at the temperature range − 37 to − 25 °C and − 12 to − 2 °C, respectively. Linoleic, oleic and palmitic acid are the major fatty acids, accounting for above 96% of the total fatty acids. The content of vitamin E, phosphatide, total phenols and sterol are 92.38–105.01 mg/100 g oil, 0.38–22.28 g/kg, 0.51–71.02 mg GAE/100 g and 161.79–533.12 mg/100 g, respectively. The solvent employed has significant effect (p < 0.05) on the thermal property, fatty acid composition and bioactive constituents of the extracted kenaf seed oil. The oil extracted with ethanol contained more nervonic acid and bioactive components such as β-carotene, phosphatide, total phenols and sterols. The introduction of ultrasound reduced the extraction time remarkably. The results demonstrate that extraction with ethanol combined with ultrasound is an effective method to extract kenaf seed oil, as more reasonable fatty acid composition and higher content of bioactive components can be achieved.

Keywords

Kenaf seed oil Extraction Ultrasound Ethanol Bioactive compound 

Notes

Acknowledgements

The work was supported financially by National Key Research and Development Plan of China (2016YFD0400205-3), Fundamental Research Funds for the Central Universities (20720190038), National Natural Science Foundation of China (31101367) and China Agriculture Research System (CARS14-1-29).

References

  1. AOCS, (1997, 2001). Ca 12–55, Aa 4–38. In: Firestone, D. (Ed.), Official Methods and Recommended Practices of the American Oil Chemists’ Society. AOCS Press, Champaign III USA.Google Scholar
  2. Bhatnagar, A.S., Krishna, A.G.G., (2013). Effect of extraction solvent on oil and bioactives composition of commercial Indian niger (Guizotia abyssinica (L.f.) Cass.) seed. Journal of the American Oil Chemists Society 90(8), 1203–1212.CrossRefGoogle Scholar
  3. Bhutada PR, Jadhav AJ, Pinjari DV, Nemade PR, Jain RD (2016) Solvent assisted extraction of oil from Moringa oleifera Lam. seeds. Ind Crops Prod 82:74–80CrossRefGoogle Scholar
  4. Chan, K.W., Ismail, M., (2009). Supercritical carbon dioxide fluid extraction of Hibiscus cannabinus L. seed oil: A potential solvent-free and high antioxidative edible oil. Food Chemistry 114(3), 970–975.CrossRefGoogle Scholar
  5. Cheng WY, Akanda JMH, Nyam KL (2016) Kenaf seed oil: A potential new source of edible oil. Trends Food Sci Technol 52:57–65CrossRefGoogle Scholar
  6. Chew SC, Tan CP, Nyam KL (2017a) Optimization of bleaching parameters in refining process of kenaf seed oil with a central composite design model. J Food Sci 82(7):1622–1630PubMedCrossRefPubMedCentralGoogle Scholar
  7. Chew SC, Tan CP, Nyam KL (2005) Optimization of degumming parameters in chemical refining process to reduce phosphorus contents in kenaf seed oil. Sep Purif Technol 188:379–385CrossRefGoogle Scholar
  8. Chew SC, Tan CP, Nyam KL (2017c) Application of response surface methodology for optimizing the deodorization parameters in chemical refining of kenaf seed oil. Sep Purif Technol 184:144–151CrossRefGoogle Scholar
  9. Clemente TE, Cahoon EB (2009) Soybean oil: Genetic approaches for modification of functionality and total content. Plant Physiol 151(3):1030–1040PubMedPubMedCentralCrossRefGoogle Scholar
  10. Coetzee, R., Labuschagne, M.T., Hugo, A., (2008). Fatty acid and oil variation in seed from kenaf (Hibiscus cannabinus L.). Industrial Crops and Products 27(1), 104–109.CrossRefGoogle Scholar
  11. Damanik M, Murkovic M (2018) The stability of palm oils during heating in a rancimat. Eur Food Res Technol 244(7):1293–1299CrossRefGoogle Scholar
  12. de Oliveira RC, Davantel de Barros ST, Gimenes ML (2013) The extraction of passion fruit oil with green solvents. J Food Eng 117(4):458–463CrossRefGoogle Scholar
  13. Dhar P, Kar CS, Ojha D, Pandey SK, Mitra J (2015) Chemistry, phytotechnology, pharmacology and nutraceutical functions of kenaf (Hibiscus cannabinus L.) and roselle (Hibiscus sabdariffa L.) seed oil: An overview. Ind Crops Prod 77:323–332CrossRefGoogle Scholar
  14. Gutfinger T (1981) Polyphenols in olive oils. J Am Oil Chem Soc 58(11):966–968CrossRefGoogle Scholar
  15. Hashemi SMB, Mousavi Khaneghah A, Akbarirad H (2015) Effects of ultrasound treatment and zenyan essential oil on lipid oxidation of blended vegetable oil. International Food Research Journal 22(5):1918–1923Google Scholar
  16. Hashemi SMB, Khaneghah AM, Akbarirad H (2016) The effects of amplitudes ultrasound-assisted solvent extraction and pretreatment time on the yield and quality of Pistacia khinjuk hull oil. J Oleo Sci 65(9):733–738CrossRefGoogle Scholar
  17. Hashemi SMB, Amarowicz R, Khaneghah AM, Vardehsara MS, Hosseini M, Yousefabad SHA (2017a) Kangar (Gundelia tehranica) seed oil: Quality measurement and frying performance. Journal of Food and Nutrition Research 56(1):86–95Google Scholar
  18. Hashemi SMB, Mousavi Khaneghah A, Koubaa M, Lopez-Cervantes J, Yousefabad SHA, Hosseini SF, Karimi M, Motazedian A, Asadifard S (2017b) Novel edible oil sources: Microwave heating and chemical properties. Food Res Int 92:147–153PubMedCrossRefGoogle Scholar
  19. ISO, (2000, 2006, 1999). ISO:5509, ISO:9936, ISO:12228. International Organization for Standardization, Geneva, SwitzerlandGoogle Scholar
  20. Khoddami, A., Ghazali, H.M., Yassoralipour, A., Ramakrishnan, Y., Ganjloo, A., (2011). Physicochemical characteristics of nigella seed (Nigella sativa L.) oil as affected by different extraction methods. Journal of the American Oil Chemists Society 88(4), 533–540.CrossRefGoogle Scholar
  21. Latif S, Anwar F (2011) Aqueous enzymatic sesame oil and protein extraction. Food Chem 125(2):679–684CrossRefGoogle Scholar
  22. Lee SY, Fu SY, Chong GH (2015) Ultrasound-assisted extraction kinetics, fatty acid profile, total phenolic content and antioxidant activity of green solvents' extracted passion fruit oil. Int J Food Sci Technol 50(8):1831–1838CrossRefGoogle Scholar
  23. Li H, Zhang Z, He D, Xia Y, Liu Q, Li X (2017) Ultrasound-assisted aqueous enzymatic extraction of oil from perilla seeds and determination of its physicochemical properties, fatty acid composition and antioxidant activity. Food Science and Technology 37:71–77CrossRefGoogle Scholar
  24. Mariod AA, Matthäus B, Ismail M (2011) Comparison of supercritical fluid and hexane extraction methods in extracting kenaf (Hibiscus cannabinus) seed oil lipids. J Am Oil Chem Soc 88(7):931–935CrossRefGoogle Scholar
  25. Mehanni, A.E.S., El-Reffaei, W.H.M., Melo, A., Casal, S., Ferreira, I.M.P.L.V.O., (2017). Enzymatic extraction of oil from Balanites Aegyptiaca (Desert Date) kernel and comparison with solvent extracted oil. Journal of Food Biochemistry 41(2), e12270, 1–6.CrossRefGoogle Scholar
  26. Mohamed A, Bhardwaj H, Hamama A, Webber C (1995) Chemical composition of kenaf (Hibiscus cannabinus L.) seed oil. Ind Crops Prod 4:157–165CrossRefGoogle Scholar
  27. Nyam KL, Tan CP, Lai OM, Long K, Man YBC (2009a) Physicochemical properties and bioactive compounds of selected seed oils. LWT-Food Science and Technology 42(8):1396–1403CrossRefGoogle Scholar
  28. Nyam KL, Tan CP, Che Man YB, Lai OM, Long K (2009b) Physicochemical properties of Kalahari melon seed oil following extractions using solvent and aqueous enzymatic methods. Int J Food Sci Technol 44(4):694–701CrossRefGoogle Scholar
  29. Oliveira R, Oliveira V, Aracava KK, Rodrigues CEDC (2012) Effects of the extraction conditions on the yield and composition of rice bran oil extracted with ethanol-A response surface approach. Food Bioprod Process 90(C1):22–31CrossRefGoogle Scholar
  30. Perrier A, Delsart C, Boussetta N, Grimi N, Citeau M, Vorobiev E (2017) Effect of ultrasound and green solvents addition on the oil extraction efficiency from rapeseed flakes. Ultrason Sonochem 39:58–65PubMedCrossRefPubMedCentralGoogle Scholar
  31. Racette SB, Lin X, Lefevre M, Spearie CA, Most MM, Ma L, Ostlund RE Jr (2010) Dose effects of dietary phytosterols on cholesterol metabolism: a controlled feeding study. Am J Clin Nutr 91(1):32–38PubMedCrossRefPubMedCentralGoogle Scholar
  32. Ranalli A, Lucera AM, Contento S, Sotiriou E (2005) Effects of processing techniques on the natural colourings and the other functional constituents in virgin olive oil. Food Res Int 38(9):873–878CrossRefGoogle Scholar
  33. Rodrigues, G.d.M., Ferreira de Mello, B.T., dos Santos Garcia, V.A., da Silva, C., (2017). Ultrasound-assisted extraction of oil from macauba pulp using alcoholic solvents. Journal of Food Process Engineering 40(5), e12530, 1–8.CrossRefGoogle Scholar
  34. Ryu, J., Kwon, S.-J., Ahn, J.-W., Jo, Y.D., Kim, S.H., Jeong, S.W., Lee, M.K., Kim, J.-B., Kang, S.-Y., (2017). Phytochemicals and antioxidant activity in the kenaf plant (Hibiscus cannabinus L.). Journal of Plant Biotechnology 44(2), 191–202.CrossRefGoogle Scholar
  35. Samaram S, Mirhosseini H, Tan CP, Ghazali HM (2014) Ultrasound-assisted extraction and solvent extraction of papaya seed oil: Crystallization and thermal behavior, saturation degree, color and oxidative stability. Ind Crops Prod 52:702–708CrossRefGoogle Scholar
  36. Samyn P, Schoukens G, Vonck L, Stanssens D, Van den Abbeele H (2012) Quality of Brazilian vegetable oils evaluated by (modulated) differential scanning calorimetry. J Therm Anal Calorim 110(3):1353–1365CrossRefGoogle Scholar
  37. Sicaire A-G, Vian MA, Fine F, Carre P, Tostain S, Chemat F (2016) Ultrasound induced green solvent extraction of oil from oleaginous seeds. Ultrason Sonochem 31:319–329PubMedCrossRefGoogle Scholar
  38. Tavakolpour, Y., Moosavi-Nasab, M., Niakousari, M., Haghighi-Manesh, S., Hashemi, S.M.B., Khaneghah, A.M., (2017). Comparison of four extraction methods for essential oil from Thymus daenensis subsp. Lancifolius and chemical analysis of extracted essential oil. Journal of Food Processing and Preservation 41(4): e13046.CrossRefGoogle Scholar
  39. Tir R, Dutta PC, Badjah-Hadj-Ahmed AY (2012) Effect of the extraction solvent polarity on the sesame seeds oil composition. Eur J Lipid Sci Technol 114(12):1427–1438CrossRefGoogle Scholar
  40. Wang L, Weller CL (2006) Recent advances in extraction of nutraceuticals from plants. Trends Food Sci Technol 17(6):300–312CrossRefGoogle Scholar
  41. Wong YH, Lau HW, Tan CP, Long K, Nyam KL (2014) Binary solvent extraction system and extraction time effects on phenolic antioxidants from kenaf seeds (Hibiscus cannabinus L.) extracted by a pulsed ultrasonic-assisted extraction. The Scientific World Journal 2014. Article 789346:1–7Google Scholar
  42. Yanty NAM, Marikkar JMN, Long K (2011) Effect of varietal differences on composition and thermal characteristics of avocado oil. J Am Oil Chem Soc 88(12):1997–2003CrossRefGoogle Scholar
  43. Yusoff MM, Gordon MH, Ezeh O, Niranjan K (2016) Aqueous enzymatic extraction of Moringa oleifera oil. Food Chem 211:400–408CrossRefGoogle Scholar
  44. Yusri, N.M., Chan, K.W., Iqbal, S., Ismail, M., (2012). Phenolic content and antioxidant activity of Hibiscus cannabinus L. seed extracts after sequential solvent extraction. Molecules 17(11), 12612–12621.PubMedPubMedCentralCrossRefGoogle Scholar
  45. Zhang ZS, Wang L-J, Li D, Jiao SS, Chen XD, Mao Z-H (2008) Ultrasound-assisted extraction of oil from flaxseed. Sep Purif Technol 62(1):192–198CrossRefGoogle Scholar
  46. Zhang Z-S, Wang L-J, Li D, Li S-J, Özkan N (2011) Characteristics of flaxseed oil from two different flax plants. Int J Food Prop 14(6):1286–1296CrossRefGoogle Scholar
  47. Zhang ZS, Li D, Zhang LX, Liu YL, Wang XD (2014) Heating effect on the DSC melting curve of flaxseed oil. J Therm Anal Calorim 115(3):2129–2135CrossRefGoogle Scholar
  48. Zheng, L., Huang, X., Wang, L., Chen, Z., (2012). Physicochemical properties, chemical composition and antioxidant activity of Dalbergia odorifera T. Chen seed oil. Journal of the American Oil Chemists Society 89(5), 883–890CrossRefGoogle Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2020

Authors and Affiliations

  1. 1.College of Food Science and TechnologyHenan University of TechnologyZhengzhouPeople’s Republic of China
  2. 2.Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical EngineeringXiamen UniversityXiamenPeople’s Republic of China

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