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Effect of ultrasonication on functional properties of tamarind seed protein isolates

  • Biswajit Biswas
  • Nandan SitEmail author
Original Article

Abstract

In the present study protein was isolated from tamarind seed powder and was subjected to ultrasonication by varying the time (15 and 30 min) and intensity (100 and 200 W) of treatment. The effect of the ultrasound treatment on the various properties like solubility, emulsifying property, foaming property, water holding capacity, oil holding capacity, particle density and molecular weight was investigated. The solubility, emulsifying property, foaming property, water holding capacity and oil holding capacity of the ultrasonically treated tamarind seed protein isolates improved after treatment and was found to increase with time or intensity of the treatment. The particle density slightly decreased after ultrasonication, but significant differences could not be observed for the different treatment conditions. The SDS-PAGE profiling did not reveal any differences in the molecular weights of the treated and untreated proteins, implying that ultrasonication did not affect the primary structure of the proteins. It can be concluded from the study that ultrasonication can be used to improve the functional properties of tamarind seed protein isolates and therefore has potential for use in various food and non-food applications.

Keywords

Tamarind seed protein isolate Ultrasonication Time Intensity Properties 

Notes

Acknowledgements

The authors greatly acknowledge the help received from Dr. Laxmikant S. Badwaik, Associate Professor, Department of Food Engineering and Technology, Tezpur University, Assam, India for allowing us to use his laboratory and equipment for carrying out the work.

References

  1. AACC American Association of Cereal Chemists (1990) Approved methods of the AACC, 7th edn. AACC American Association of Cereal Chemists, St. Paul (Method 46-12 (Protein)) Google Scholar
  2. AOAC Official Methods of Analysis (1990) Association of Official Analytical Chemists, 15th edn. Washington, DC, USAGoogle Scholar
  3. Arzeni C, Martínez K, Zema P, Arias A, Pérez OE, Pilosof AMR (2012) Comparative study of high intensity ultrasound effects on food proteins functionality. J Food Eng 108(3):463–472CrossRefGoogle Scholar
  4. Bagul Mayuri, Sonawane Sachin K, Arya Shalini S (2015) Tamarind seeds: chemistry, technology, applications and health benefits: a review. Indian Food Ind Mag 34(3):28–35Google Scholar
  5. Barac M, Cabrilo S, Pesic M, Stanojevic S, Zilic S, Macej O, Ristic N (2010) Profile and functional properties of seed proteins from six pea (Pisum sativum) genotypes. Int J Mol Sci 11(12):4973–4990PubMedPubMedCentralCrossRefGoogle Scholar
  6. Bhattacharya S, Bal S, Mukherjee RK, Bhattacharya S (1994) Functional and nutritional properties of tamarind (Tamarindus indica) kernel protein. Food Chem 49(1):1–9CrossRefGoogle Scholar
  7. Butt MS, Batool R (2010) Nutritional and functional properties of some promising legumes protein isolates. Pak J Nutr 9(4):373–379CrossRefGoogle Scholar
  8. Chant SR (1993) Fables. In: Heywood VH (ed) Flowering plants of the world. B.T. Batsford Ltd., LondonGoogle Scholar
  9. Frydenberg RP, Hammershøj M, Andersen U, Greve MT, Wiking L (2016) Protein denaturation of whey protein isolates (WPIs) induced by high intensity ultrasound during heat gelation. Food Chem 192:415–423PubMedCrossRefGoogle Scholar
  10. Golly MK, Ma H, Yuqing D, Wu P, Dabbour M, Sarpong F, Farooq M (2019) Enzymolysis of walnut (Juglans regia L.) meal protein: ultrasonication-assisted alkaline pretreatment impact on kinetics and thermodynamics. J Food Biochem 43(8):e12948PubMedCrossRefGoogle Scholar
  11. Horax R, Hettiarachchy NS, Chen P, Jalaluddin M (2004) Functional properties of protein isolate from cowpea (Vigna unguiculata L. Walp.). J Food Sci 69(2):fct119–fct121Google Scholar
  12. Hu H, Li-Chan EC, Wan L, Tian M, Pan S (2013a) The effect of high intensity ultrasonic pre-treatment on the properties of soybean protein isolate gel induced by calcium sulfate. Food Hydrocoll 32(2):303–311CrossRefGoogle Scholar
  13. Hu H, Wu J, Li-Chan EC, Zhu L, Zhang F, Xu X, Fan G, Wang L, Huang X, Pan S (2013b) Effects of ultrasound on structural and physical properties of soy protein isolate (SPI) dispersions. Food Hydrocoll 30(2):647–655CrossRefGoogle Scholar
  14. Jambrak AR, Mason TJ, Lelas V, Herceg Z, Herceg IL (2008) Effect of ultrasound treatment on solubility and foaming properties of whey protein suspensions. J Food Eng 86(2):281–287CrossRefGoogle Scholar
  15. Jambrak AR, Lelas V, Mason TJ, Krešić G, Badanjak M (2009) Physical properties of ultrasound treated soy proteins. J Food Eng 93(4):386–393CrossRefGoogle Scholar
  16. Jiang L, Wang J, Li Y, Wang Z, Liang J, Wang R, Chen Y, Ma W, Qi B, Zhang M (2014) Effects of ultrasound on the structure and physical properties of black bean protein isolates. Food Res Int 62:595–601CrossRefGoogle Scholar
  17. Jitngarmkusol S, Hongsuwankul J, Tananuwong K (2008) Chemical compositions, functional properties, and microstructure of defatted macadamia flours. Food Chem 110(1):23–30PubMedCrossRefGoogle Scholar
  18. Krešić G, Lelas V, Jambrak AR, Herceg Z, Brnčić SR (2008) Influence of novel food processing technologies on the rheological and thermophysical properties of whey proteins. J Food Eng 87(1):64–73CrossRefGoogle Scholar
  19. Kumar CS, Bhattacharya S (2008) Tamarind seed: properties, processing and utilization. Crit Rev Food Sci Nutr 48(1):1–20PubMedCrossRefGoogle Scholar
  20. Lam RS, Nickerson MT (2013) Food proteins: a review on their emulsifying properties using a structure–function approach. Food Chem 141(2):975–984PubMedCrossRefGoogle Scholar
  21. Lin MJY, Humbert ES, Sosulski FW (1974) Certain functional properties of sunflower meal products. J Food Sci 39(2):368–370CrossRefGoogle Scholar
  22. Menezes BS, Zanette B, Souza JTA, Cortez-Vega WR, Prentice C (2015) Comparison of physicochemical and functional properties of surimi and protein isolate obtained from mechanically deboned meat of chicken. Int Food Res J 22(4):1374–1379Google Scholar
  23. Mirmoghtadaie L, Aliabadi SS, Hosseini SM (2016) Recent approaches in physical modification of protein functionality. Food Chem 199:619–627PubMedCrossRefGoogle Scholar
  24. Moulton KJ, Wang LC (1982) A pilot-plant study of continuous ultrasonic extraction of soybean protein. J Food Sci 47(4):1127–1129CrossRefGoogle Scholar
  25. Naczk M, Diosady LL, Rubin LJ (1985) Functional properties of canola meals produced by a two-phase solvent extraction system. J Food Sci 50(6):1685–1688CrossRefGoogle Scholar
  26. O’Sullivan J, Murray B, Flynn C, Norton I (2016) The effect of ultrasound treatment on the structural, physical and emulsifying properties of animal and vegetable proteins. Food Hydrocoll 53:141–154CrossRefGoogle Scholar
  27. Peleg M (1983) Physical properties of foods. In: Peleg M, Bagley EB (eds) IFT basic symposium series (USA). AVI Publishing Co., Inc., West-Port, pp 293–321Google Scholar
  28. Sarkar A, Kamaruddin H, Bentley A, Wang S (2016) Emulsion stabilization by tomato seed protein isolate: influence of pH, ionic strength and thermal treatment. Food Hydrocoll 57:160–168CrossRefGoogle Scholar
  29. Sarv V, Trass O, Diosady LL (2017) Preparation and characterization of Camelina sativa protein isolates and mucilage. J Am Oil Chem Soc 94(10):1279–1285CrossRefGoogle Scholar
  30. Singh A, Benjakul S, Kijroongrojana K (2018) Effect of ultrasonication on physicochemical and foaming properties of squid ovary powder. Food Hydrocoll 77:286–296CrossRefGoogle Scholar
  31. Smith AK, Circle CJ (1939) Soy bean protein precipitation from water and alkaline dispersions by acids and by electrodialysis. Ind Eng Chem 31:1284–1288CrossRefGoogle Scholar
  32. Sosulski F, Humbert ES, Bui K, Jones JD (1976) Functional propreties of rapeseed flours, concentrates and isolate. J Food Sci 41(6):1349–1352CrossRefGoogle Scholar
  33. Stefanović A, Jovanović J, Dojčinović M, Lević S, Žuža M, Nedović V, Knežević-Jugović Z (2014) Impact of high-intensity ultrasound probe on the functionality of egg white proteins. J Hyg Eng Des 6:215–224Google Scholar
  34. Wu W, Hua Y, Lin Q, Xiao H (2011) Effects of oxidative modification on thermal aggregation and gel properties of soy protein by peroxyl radicals. Int J Food Sci Technol 46(9):1891–1897CrossRefGoogle Scholar
  35. Yanjun S, Jianhang C, Shuwen Z, Hongjuan L, Jing L, Lu L, Uluko H, Yanling S, Wenming C, Wupeng G, Jiaping L (2014) Effect of power ultrasound pre-treatment on the physical and functional properties of reconstituted milk protein concentrate. J Food Eng 124:11–18CrossRefGoogle Scholar
  36. Zhang H, Claver IP, Zhu KX, Zhou H (2011) The effect of ultrasound on the functional properties of wheat gluten. Molecules 16(5):4231–4240PubMedCentralCrossRefGoogle Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2020

Authors and Affiliations

  1. 1.Department of Food Engineering and TechnologyTezpur UniversityTezpurIndia

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