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Characterization of fish sausage manufactured with combination of sunflower oil and fish oil stabilized with fish roe protein hydrolysates

  • Mona Hajfathalian
  • Sarah Jorjani
  • Sakhi GhelichiEmail author
Original Article
  • 11 Downloads

Abstract

The present study aimed to determine the effect of adding protein hydrolysates obtained after 30, 60, and 90 min enzymatic hydrolysis of fish roe on properties of silver carp sausages enriched with fish oil during storage at 4 °C for 30 days. Properties of the fortified sausages were determined by assessment of primary and secondary oxidation, fatty acid composition, microbial spoilage, texture, and organoleptic properties. The results indicated that the hydrolysates could retard oil oxidation and microbial spoilage and preserve n 3 fatty acids in fish sausages during the refrigerated storage. Also, they rendered firmer microstructure with smaller oil droplets to the sausages. Fish sausages fortified with the hydrolysates were lighter and exhibited better textural and sensory properties. It can be concluded that enzymatic hydrolysates from discarded fish roe can be added to fish sausages containing fish oil to retard oil oxidation and microbial spoilage and improve sausage properties.

Keywords

Silver carp sausage Fish roe hydrolysate Fish oil Sausage properties Oxidation 

Notes

Acknowledgements

We are thankful to Gorgan University of Agricultural Sciences and Natural Resources and Islamic Azad University of Iran to support this research. We are also very grateful to Professor Charlotte Jacobsen from Technical University of Denmark for her kind support.

Supplementary material

13197_2019_4179_MOESM1_ESM.docx (15 kb)
Supplementary material 1 (DOCX 14 kb)

References

  1. AOAC (2006) Official methods of analysis, 18th edn. Association of Official Analytical Chemists Inc, ArlingtonGoogle Scholar
  2. Balaswamy K, Rao PGP, Rao GN, Rao DG, Jyothirmayi T (2009) Physicochemical composition and functional properties of roes from some fresh water fish species and their application in some foods. J Environ Agric Food Chem 8(8):704–710Google Scholar
  3. Bligh EG, Dyer WJ (1959) A rapid method for total lipid extraction and purification. Can J Biochem Physiol 37:911–917CrossRefGoogle Scholar
  4. Buege JA, Aust SD (1978) Microsomal lipid peroxidation. Methods Enzymol 52:302–310CrossRefGoogle Scholar
  5. Cáceres E, García ML, Selgas MD (2008) Effect of pre-emulsified fish oil—as source of PUFA n-3-on microstructure and sensory properties of mortadella, a Spanish bologna-type sausage. Meat Sci 80:183–193CrossRefGoogle Scholar
  6. Chalamaiah M, Dinesh Kumar B, Hemalatha R, Jyothirmayi T (2012) Fish protein hydrolysates: proximate composition, amino acid composition, antioxidant activities and applications: a review. Food Chem 135:3020–3038CrossRefGoogle Scholar
  7. Chalamaiah M, Jyothirmayi T, Diwan PV, Kumar BD (2015) Antioxidant activity and functional properties of enzymatic protein hydrolysates from common carp (Cyprinus carpio) roe (egg). J Food Sci Technol 52(9):5817–5825CrossRefGoogle Scholar
  8. Chen L, Wang P, Kang ZL, Li K, Xie C, Sun JX, Xu XL (2015) Effect of soybean oil emulsified and unemulsified with chicken plasma protein on the physicochemical properties of frankfurters. CyTA J Food 13(3):445–455CrossRefGoogle Scholar
  9. Chi CF, Wang B, Wang YM, Zhang B, Deng SG (2015) Isolation and characterisation of three antioxidant peptides from protein hydrolysate of bluefin leatherjacket (Navodon septentrionalis) heads. J Funct Foods 12:1–10CrossRefGoogle Scholar
  10. Elias RJ, Kellerby SS, Decker EA (2008) Antioxidant activity of proteins and peptides. Crit Rev Food Sci Nutr 48:430–441CrossRefGoogle Scholar
  11. Ennaas N, Hammami R, Beaulieu L, Fliss I (2015) Purification and characterisation of four antibacterial peptides from protamex hydrolysate of Atlantic mackerel (Scomber scombrus) by-products. Biochem Biophys Res Commun 462:195–200CrossRefGoogle Scholar
  12. Feng T, Ye R, Zhuang H, Rong Z, Fang Z, Wang Y, Gu Z, Jin Z (2013) Physicochemical properties and sensory evaluation of Mesona Blumes gum/rice starch mixed gels as fat-substitutes in Chinese Cantonese-style sausage. Food Res Int 50:85–93CrossRefGoogle Scholar
  13. Galla NR, Pamidighantam PR, Akula S, Karakala B (2012) Functional properties and in vitro antioxidant activity of roe protein hydrolysates of Channa striatus and Labeo rohita. Food Chem 135:1479–1484CrossRefGoogle Scholar
  14. García-Moreno PJ, Guadix A, Guadix EM, Jacobsen C (2016) Physical and oxidative stability of fish oil-in-water emulsions stabilized with fish protein hydrolysates. Food Chem 203:124–135CrossRefGoogle Scholar
  15. Intarasirisawat R, Benjakul S, Visessanguan W (2011) Chemical compositions of the roes from skipjack, tongol and bonito. Food Chem 124:1328–1334CrossRefGoogle Scholar
  16. Intarasirisawat R, Benjakul S, Visessanguan W, Wu J (2014) Effects of skipjack roe protein hydrolysate on properties and oxidative stability of fish emulsion sausage. LWT Food Sci Technol 58:280–286CrossRefGoogle Scholar
  17. Jemil I, Jridi M, Nasri R, Ktari N, Ben Slama-Ben Salem R, Mehiri M, Hajji M, Nasri M (2014) Functional, antioxidant and antibacterial properties of protein hydrolysates prepared from fish meat fermented by Bacillus subtilis A26. Process Biochem 49:963–972CrossRefGoogle Scholar
  18. Kjeldahl J (1883) Neue Methode zur Bestimmung des Stickstoffs in organischen Körpern (New method for the determination of nitrogen in organic substances). Z Anal Chem 22(1):366–383CrossRefGoogle Scholar
  19. Kristinsson HG, Rasco BA (2000) Fish protein hydrolysates: production, biochemical, and functional properties. Crit Rev Food Sci Nutr 40:43–81CrossRefGoogle Scholar
  20. Linde GA, Junior AL, de Faria EV, Colauto NB, de Moraes FF, Zanin GM (2009) Taste modification of amino acids and protein hydrolysate by α-cyclodextrin. Food Res Int 42:814–818CrossRefGoogle Scholar
  21. Lorenzo JM, González-Rodríguez RM, Sánchez M, Amado IR, Franco D (2013) Effects of natural (grape seed and chestnut extract) and synthetic antioxidants (buthylatedhydroxytoluene, BHT) on the physical, chemical, microbiological and sensory characteristics of dry cured sausage “chorizo”. Food Res Int 54:611–620CrossRefGoogle Scholar
  22. Maqsood S, Benjakul S, Balange AK (2012) Effect of tannic acid and kiam wood extract on lipid oxidation and textural properties of fish emulsion sausages during refrigerated storage. Food Chem 130:408–416CrossRefGoogle Scholar
  23. Morales-Medina R, Tamm F, Guadix A, Guadix EM, Drusch S (2016) Functional and antioxidant properties of hydrolysates of sardine (S. pilchardus) and horse mackerel (T. mediterraneus) for the microencapsulation of fish oil by spray-drying. Food Chem 194:1208–1216CrossRefGoogle Scholar
  24. Nasri R, Younes I, Jridi M, Trigui M, Bougatef A, Nedjar-Arroume N, Dhulster P, Nasri M, Karra-Châabouni M (2013) ACE inhibitory and antioxidative activities of Goby (Zosterissessor ophiocephalus) fish protein hydrolysates: effect on meat lipid oxidation. Food Res Int 54:552–561CrossRefGoogle Scholar
  25. Nikoo M, Benjakul S, Xu X (2015) Antioxidant and cryoprotective effects of Amur sturgeon skin gelatin hydrolysate in unwashed fish mince. Food Chem 181:295–303CrossRefGoogle Scholar
  26. Ovissipour M, Abedian A, Motamedzadegan A, Rasco B, Safari R (2009) The effect of enzymatic hydrolysis time and temperature on the properties of protein hydrolysates from Persian sturgeon (Acipenser persicus) viscera. Food Chem 115:238–242CrossRefGoogle Scholar
  27. Pu C, Tang W (2017) Affinity and selectivity of anchovy antibacterial peptide for Staphylococcus aureus cell membrane lipid and its application in whole milk. Food Control 72:153–163CrossRefGoogle Scholar
  28. Rahali V, Chobert JM, Haertle T, Gueguen J (2000) Emulsification of chemical and enzymatic hydrolysates of β-lactoglobulin: characterisation of the peptides adsorbed at the interface. Nahrung 44:89–95CrossRefGoogle Scholar
  29. Shantha NC, Decker EA (1994) Rapid, sensitive, iron-based spectrophotometric methods for determination of peroxide values of food lipids. J AOAC Int 77:421–424PubMedGoogle Scholar
  30. Sila A, Nedjar-Arroume N, Hedhili K, Chataigné G, Balti R, Nasri M, Dhulster P, Bougatef A (2014) Antibacterial peptides from barbel muscle protein hydrolysates: activity against some pathogenic bacteria. LWT Food Sci Technol 55:183–188CrossRefGoogle Scholar
  31. Sokmen M, Serkedjieva J, Daferera D, Gulluce M, Polissiou M, Tepe B, Akpulat HA, Sahin F, Sokmen A (2004) In vitro antioxidant, antimicrobial, and antiviral activities of the essential oil and various extracts from herbal parts and callus cultures of Origanum acutidens. J Agric Food Chem 52:3309–3312CrossRefGoogle Scholar
  32. Souissi N, Jridi M, Nasri R, Ben Slama R, Njeh M, Nasri M (2016) Effects of the edible cuttlefish gelatin on textural, sensorial and physicochemical quality of octopus sausage. LWT Food Sci Technol 65:18–24CrossRefGoogle Scholar
  33. Stannard C (1997) Development and use of microbiological criteria for foods. Food Sci Technol Today 11(3):137–177Google Scholar
  34. Tayel AA (2016) Microbial chitosan as a biopreservative for fish sausages. Int J Biol Macromol 93:41–46CrossRefGoogle Scholar
  35. Youssef MK, Barbut S (2011) Fat reduction in comminuted meat products-effects of beef fat, regular and pre-emulsified canola oil. Meat Sci 87:356–360CrossRefGoogle Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2019

Authors and Affiliations

  1. 1.Young Researchers and Elite Club, Gorgan BranchIslamic Azad UniversityGorgānIran
  2. 2.Department of Fisheries, Azadshahr BranchIslamic Azad UniversityAzadshahrIran
  3. 3.Department of Seafood Science and Technology, Faculty of Fisheries and Environmental ScienceGorgan University of Agricultural Sciences and Natural ResourcesGorgānIran

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