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Effect of dynamic ultra-high pressure homogenization on the structure and functional properties of whey protein

  • Chunyan Wang
  • Jianan Wang
  • Dongyang Zhu
  • Shengjie Hu
  • Zhuangli Kang
  • Hanjun MaEmail author
Original Article
  • 16 Downloads

Abstract

The effects of dynamic ultra-high pressure homogenization (UHPH) on the structure and functional properties of whey protein were investigated in this study. Whey protein solution of 10 mg/mL (1% w/w) was prepared and processed by a laboratory scale high pressure homogenizer with different pressures (25, 50, 100, 150, 200, and 250 MPa) at an initial temperature of 25 °C. Then, the solution samples were evaluated in terms of secondary structure, sulfhydryl and disulfide bond contents, surface hydrophobicity, average particle size, solubility, foaming capacity, emulsifying activity, and thermal properties. It was found that the secondary structure of whey protein changed with the dynamic UHPH treatment. The interchange reaction between the disulfide bond and the sulfhydryl group was promoted and the surface hydrophobicity significantly increased. The functional properties of the whey protein accordingly changed. Specifically, after dynamic UHPH treatment, the average particle size of the whey protein and emulsion decreased while the solubility, the foaming capability and the emulsification stability increased significantly. The results also revealed that with the dynamic UHPH at 150 MPa, the best improvement was observed in the whey protein functional properties. The whey protein solubility increased from 63.15 to 71.61% and the emulsification stability improved from 195 to 467 min.

Keywords

Ultra-high pressure homogenization Whey protein Structure Functional properties 

Notes

Acknowledgements

This research was supported by the National Natural Science Foundation of China (No. 31571912) and the Major Science and Technology Project in Henan (No. 161100110600).

References

  1. Blayo C, Vidcoqauthor O, Dumayauthor E (2016) Effects of high pressure processing (hydrostatic high pressure and ultra-high pressure homogenisation) on whey protein native state and susceptibility to tryptic hydrolysis at atmospheric pressure. Food Res Int 79:40–53CrossRefGoogle Scholar
  2. Calligaris S, Plazzotta S, Valoppi F, Anese M (2018) Combined high-power ultrasound and high-pressure homogenization nanoemulsification: the effect of energy density, oil content and emulsifier type and content. Food Res Int 107:700–707CrossRefGoogle Scholar
  3. Cheng J, Ren W, Wang S, Zhang Z, Zhao W, College F (2014) Effect of physical treatments on soluble protein of highly denatured defatted soy flakes. J Food Sci Technol 45:1253–1260Google Scholar
  4. Corzomartínez M, Mohan M, Dunlap J, Harte F (2015) Effect of ultra-high pressure homogenization on the interaction between bovine casein micelles and ritonavir. Pharm Res 32:1055–1071CrossRefGoogle Scholar
  5. Ding Y, Kan J (2017) Optimization and characterization of high pressure homogenization produced chemically modified starch nanoparticles. J Food Sci Technol 54:4501–4509CrossRefGoogle Scholar
  6. Dissanayake M, Ramchandran L, Piyadasa C, Vasiljevic T (2013) Influence of heat and pH on structure and conformation of whey proteins. Int Dairy J 28:56–61CrossRefGoogle Scholar
  7. Fasinu EG, Ikhu-Omoregbe DIO, Jideani VA (2015) Influence of selected physicochemical factors on the stability of emulsions stabilized by bambara groundnut flour and starch. J Food Sci Technol 52:7048–7058CrossRefGoogle Scholar
  8. Floury J, Desrumaux A, Axelos MAV, Legrand J (2002) Degradation of methylcellulose during ultra-high-pressure homogenization. J Food Hydrocol 16:47–53CrossRefGoogle Scholar
  9. Friedman M (1994) Improvement in the safety of foods by sulfhydryl-containing amino acids and peptides. A review. J Agric Food Chem 42:3–20CrossRefGoogle Scholar
  10. Hua X, Xu S, Wang M, Chen Y, Yang H, Yang R (2017) Effects of high-speed homogenization and high-pressure homogenization on structure of tomato residue fibers. Food Chem 232:443–449CrossRefGoogle Scholar
  11. Jambrak AR, Mason TJ, Lelas V, Herceg Z, Herceg IL (2008) Effect of ultrasound treatment on solubility and foaming properties of whey protein suspensions. J Food Eng 86:281–287CrossRefGoogle Scholar
  12. Kitabatake N, Doi E (2015) Surface tension and foaming of protein solutions. J Food Sci 47:1218–1221CrossRefGoogle Scholar
  13. Le TT, Bhandari B, Holland JW, Deeth HC (2011) Maillard reaction and protein cross-linking in relation to the solubility of milk powders. J Agric Food Chem 59:12473–12479CrossRefGoogle Scholar
  14. Lin D, Zhou W, Zhao J, Lan W, Chen R, Li Y (2017) Study on the synthesis and physicochemical properties of starch acetate with low substitution under microwave assistance. Int J Biol Macromol 103:316–326CrossRefGoogle Scholar
  15. Lin D, Zhou W, He Q, Xing B, Wu Z, Chen H (2019a) Study on preparation and physicochemical properties of hydroxypropylated starch with different degree of substitution under microwave assistance. Int J Biol Macromol 125:290–299CrossRefGoogle Scholar
  16. Lin D, Zhou W, Yang Z (2019b) Study on physicochemical properties, digestive properties and application of acetylated starch in noodles. Int J Biol Macromol 128:948–956CrossRefGoogle Scholar
  17. Liu LL, Wang H, Ren GY, Xu D, Dan L, Yin GJ (2015) Effects of freeze-drying and spray drying processes on functional properties of phosphorylation of egg white protein. Int J Agric Biol Eng 8:116–123Google Scholar
  18. Mao XY, Hua YF (2014) Chemical composition, molecular weight distribution, secondary structure and effect of nacl on functional properties of walnut (Juglans regia L.) protein isolates and concentrates. J Food Sci Technol 51:1473–1482CrossRefGoogle Scholar
  19. Oliveira CF, Corrêa AP, Coletto D, Daroit DJ, Cladera-Olivera F, Brandelli A (2015) Soy protein hydrolysis with microbial protease to improve antioxidant and functional properties. J Food Sci Technol 52:2668–2678CrossRefGoogle Scholar
  20. Perreault V, Hénaux L, Bazinet L, Doyen A (2017) Pretreatment of flaxseed protein isolate by high hydrostatic pressure: impacts on protein structure, enzymatic hydrolysis and final hydrolysate antioxidant capacities. Food Chem 221:1805–1812CrossRefGoogle Scholar
  21. Plancken IVD, Loey AV, Hendrickx ME (2007) Foaming properties of egg white proteins affected by heat or high pressure treatment. J Food Eng 78:1410–1426CrossRefGoogle Scholar
  22. Riebroy S, Benjakul S, Visessanguan W, Erikson U, Rustad T (2009) Acid-induced gelation of natural actomyosin from atlantic cod (Gadus morhua) and burbot (Lota lota). J Food Hydrocoll 23:26–39CrossRefGoogle Scholar
  23. Sano T, Ohno T, Otsukafuchino H, Matsumoto JJ, Tsuchiya T (2010) Carp natural actomyosin: thermal denaturation mechanism. J Food Sci 59:1002–1008CrossRefGoogle Scholar
  24. Sen M, Kopper R, Pons L, Abraham EC, Burks AW, Bannon GA (2002) Protein structure plays a critical role in peanut allergen stability and may determine immunodominant IgE-binding epitopes. J Immunol 169:882–887CrossRefGoogle Scholar
  25. Sørensen H, Mortensen K, Sørland GH, Larsen FH, Paulsson M, Ipsen R (2015) Dynamic ultra-high pressure homogenisation of whey protein-depleted milk concentrate. J Int Dairy J 46:12–21CrossRefGoogle Scholar
  26. Wang CY, Ma YP, Liu BG, Kang ZL, Geng S, Wang JN (2019) Effects of dynamic ultra-high pressure homogenization on the structure and functional properties of casein. Int J Agric Biol Eng 12:229–234CrossRefGoogle Scholar
  27. Wu Z, Huang Y, Xiao L, Lin D, Yang Y, Wang H (2019) Physical properties and structural characterization of starch/polyvinyl alcohol/graphene oxide composite films. Int J Biol Macromol 123:569–575CrossRefGoogle Scholar
  28. Xi J, He M (2018) High hydrostatic pressure (hhp) effects on antigenicity and structural properties of soybean β-conglycinin. J Food Sci Technol 55:630–637CrossRefGoogle Scholar
  29. Yan B, Park SH, Balasubramaniam VM (2017) Influence of high pressure homogenization with and without lecithin on particle size and physicochemical properties of whey protein-based emulsions. J Food Process Eng 40:e12578CrossRefGoogle Scholar
  30. Yin SW, Tang CH, Cao JS, Hu EK, Wen QB, Yang XQ (2008) Effects of limited enzymatic hydrolysis with trypsin on the functional properties of hemp (Cannabis sativa L.) protein isolate. Food Chem 106:1004–1013CrossRefGoogle Scholar
  31. Zhang X, Haque ZZ (2015) Generation and stabilization of whey based monodispered nanoemulsions using ultra-high pressure homogenization and small amphipathic co-emulsifier combinations. J Agric Food Chem 63:10070–10077CrossRefGoogle Scholar
  32. Zhang J, Rui D, Tian Y, Konno K (2009) Characterisation of acid-soluble collagen from skin of silver carp (Hypophthalmichthys molitrix). Food Chem 116:318–322CrossRefGoogle Scholar
  33. Zhang Z, Regenstein JM, Zhou P, Yang Y (2017) Effects of high intensity ultrasound modification on physicochemical property and water in myofibrillar protein gel. Ultrasonics Sonochem 34:960–967CrossRefGoogle Scholar
  34. Zhao Q, Selomulya C, Xiong H, Chen XD, Xia R, Wang S, Xie J, Peng H, Sun W, Zhou Q (2012) Comparison of functional and structural properties of native and industrial process-modified proteins from long-grain indica rice. J Cereal Sci 56:568–575CrossRefGoogle Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2019

Authors and Affiliations

  • Chunyan Wang
    • 1
  • Jianan Wang
    • 1
  • Dongyang Zhu
    • 1
  • Shengjie Hu
    • 1
  • Zhuangli Kang
    • 1
  • Hanjun Ma
    • 1
    Email author
  1. 1.School of Food ScienceHenan Institute of Science and TechnologyXinxiangChina

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