Chinese quince seed proteins: sequential extraction processing and fraction characterization

  • Yejun Deng
  • Lixin HuangEmail author
  • Caihong Zhang
  • Pujun Xie
Original Article


Chinese quince seed proteins were sequentially extracted based on the modified Osborne method. Investigations showed that albumin and glutelin were the major fractions. The physicochemical and functional properties of these two fractions were determined. The results showed that both albumin and glutelin posed appropriate essential amino acid composition and met the minimum recommendation (World Health Organization/Food and Agriculture Organization) for adult diet, except for methionine. The hydrophobicity of albumin and glutelin were 1063.56 and 1170.21, respectively. According to differential scanning calorimeter analysis, the denaturation temperature of albumin and glutelin was 101.44 °C and 108.36 °C respectively, and the glutelin fraction had a better thermal stability. The solubility and apparent viscosity of albumin and glutelin were presented to be greatly influenced by pH values. The water holding capacity and oil adsorption capacity of glutelin were 5.44 g/g and 8.15 g/g, higher than those of albumin which were 3.76 g/g and 3.71 g/g, respectively. Circular dichroism determination revealed albumin and glutelin were mainly composed by α-helix and random coil structures. Albumin and glutelin presented the potential as favorable nutrition and functional additive in food industries.


Chinese quince seed Albumin Glutelin Amino acid composition Functional properties 



This study was founded by the Jiangsu provincial natural science foundation of China [grant number BK20181124].

Compliance with ethical standards

Conflict of interest



  1. Adebiyi AP, Aluko RE (2011) Functional properties of protein fractions obtained from commercial yellow field pea (pisum sativum l.) seed protein isolate. Food Chem 128:902–908CrossRefGoogle Scholar
  2. Adebiyi AP, Adebiyi AO, Ogawa T, Muramoto K (2010) Preparation and characterization of high-quality rice bran proteins. J Sci Food Agric 87:1219–1227CrossRefGoogle Scholar
  3. Ajibola CF, Malomo SA, Fagbemi TN, Aluko RE (2016) Polypeptide composition and functional properties of African yam bean seed (Sphenostylis stenocarpa) albumin, globulin and protein concentrate. Food Hydrocoll 56:189–200CrossRefGoogle Scholar
  4. Aletor O, Oshodi AA, Ipinmoroti K (2002) Chemical composition of common leafy vegetables and functional properties of their leaf protein concentrates. Food Chem 78:63–68CrossRefGoogle Scholar
  5. Amza T, Amadou I, Zhu K, Zhou H (2011) Effect of extraction and isolation on physicochemical and functional properties of an underutilized seed protein: gingerbread plum (neocarya macrophylla). Food Res Int 44:2843–2850CrossRefGoogle Scholar
  6. Angel SSD, Martínez EM, López MAV (2003) Study of denaturation of corn proteins during storage using differential scanning calorimetry. Food Chem 83:531–540CrossRefGoogle Scholar
  7. Beuchat LR (1977) Functional and electrophoretic characteristics of succinylated peanut flour protein. J Agric Food Chem 25:258–261CrossRefGoogle Scholar
  8. Beveridge T, Toma SJ, Nakai S (2010) Determination of SH-and SS- groups in some food proteins using Ellman’s reagent. J Food Sci 39:49–51CrossRefGoogle Scholar
  9. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254CrossRefPubMedPubMedCentralGoogle Scholar
  10. Cepeda E, Villaran MC, Aranguiz N (1998) Functional properties of faba bean (Vicia faba) protein flour dried by spray drying and freeze drying. J Food Eng 36:303–310CrossRefGoogle Scholar
  11. Chen S, Paredes-Lopez O (2010) Isolation and characterization of the 11 s globulin from amaranth seeds. J Food Biochem 21:53–65CrossRefGoogle Scholar
  12. Chen J, Mu T, Zhang M et al (2018) Structure, physicochemical and functional properties of protein isolates and major fractions from cumin (Cuminum cyminum) seeds. Int J Food Prop 21:685–701CrossRefGoogle Scholar
  13. Deng Q, Wang L, Wei F et al (2011) Functional properties of protein isolates, globulin and albumin extracted from Ginkgo biloba seeds. Food Chem 124:1458–1465CrossRefGoogle Scholar
  14. Deng Y, Huang L, Zhang C, Xie P, Cheng J, Wang X, Li S (2019) Physicochemical and functional properties of Chinese quince seed protein isolate. Food Chem 283:539–548CrossRefPubMedGoogle Scholar
  15. Du Y, Jiang Y, Zhu X et al (2012) Physicochemical and functional properties of the protein isolate and major fractions prepared from Akebia trifoliata var. australis seed. Food Chem 133:923–929CrossRefGoogle Scholar
  16. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1980) Colorimetric method for determination of sugars and related substances. Anal Chem 89:449–454Google Scholar
  17. Eladawy TA (2000) Functional properties and nutritional quality of acetylated and succinylated mung bean protein isolate. Food Chem 70:83–91CrossRefGoogle Scholar
  18. Gao LL, Li YQ, Wang ZS et al (2018) Physicochemical characteristics and functionality of tree peony (Paeonia suffruticosa Andr.) seed protein. Food Chem 240:980–988CrossRefPubMedGoogle Scholar
  19. Gazzola D, Vincenzi S, Gastaldon L et al (2014) The proteins of the grape (Vitis vinifera l.) seed endosperm: fractionation and identification of the major components. Food Chem 155:132–139CrossRefPubMedGoogle Scholar
  20. Hu XZ, Cheng YQ, Fan JF et al (2010) Effects of drying method on physicochemical and functional properties of soy protein isolates. J. Food Process Pres 34:520–540CrossRefGoogle Scholar
  21. Idouraine A, Yensen SB, Weber CW (2010) Tepary bean flour, albumin and globulin fractions functional properties compared with soy protein isolate. J Food Sci 56:1316–1318CrossRefGoogle Scholar
  22. Kato A, Nakai S (1980) Hydrophobicity determined by a fluorescence probe method and its correlation with surface properties of proteins. Biochim Biophys Acta 624:13–20CrossRefPubMedGoogle Scholar
  23. Lawal OS, Adebowale KO, Ogunsanwo BM, Sosanwo OA, Bankole SA (2005) On the functional properties of globulin and albumin protein fractions and flours of African locust bean (Parkia biglobossa). Food Chem 92:681–691CrossRefGoogle Scholar
  24. Malomo SA, Aluko RE (2015) Conversion of a low protein hemp seed meal into a functional protein concentrate through enzymatic digestion of fibre coupled with membrane ultrafiltration. Innov Food Sci Emerg 31:151–159CrossRefGoogle Scholar
  25. Myers DJ, Hojilla-Evangelista MP, Johnson LA (1994) Functional properties of protein extracted from flaked, defatted, whole corn by ethanol/alkali during sequential extraction processing. J Am Oil Chem Soc 71:1201–1204CrossRefGoogle Scholar
  26. Ragab DM, Babiker EE, Eltinay AH (2004) Fractionation, solubility and functional properties of cowpea (Vigna unguiculata) proteins as affected by ph and/or salt concentration. Food Chem 84:207–212CrossRefGoogle Scholar
  27. Rezig L, Chibani F, Chouaibi M, Dalgalarrondo M, Hessini K, Hamdi S (2013) Pumpkin (Cucurbita maxima) seed proteins: sequential extraction processing and fraction characterization. J Agric Food Chem 61:7715–7721CrossRefPubMedGoogle Scholar
  28. Sandovaloliveros MR, Paredeslópez O (2013) Isolation and characterization of proteins from chia seeds (Salvia hispanica l.). J Agric Food Chem 61:193–201CrossRefGoogle Scholar
  29. Shchekoldina T, Aider M (2014) Production of low chlorogenic and caffeic acid containing sunflower meal protein isolate and its use in functional wheat bread making. J Food Sci Technol 51:2331–2343CrossRefPubMedGoogle Scholar
  30. Shevkani K, Singh N, Kaur A, Rana JC (2015) Structural and functional characterization of kidney bean and field pea protein isolates: a comparative study. Food Hydrocoll 43:679–689CrossRefGoogle Scholar
  31. Siow HL, Gan CY (2014) Functional protein from cumin seed (Cuminum cyminum): optimization and characterization studies. Food Hydrocoll 41:178–187CrossRefGoogle Scholar
  32. Spies JR (1967) Determination of tryptophan in protein. Anal Chem 39:1412–1416CrossRefPubMedGoogle Scholar
  33. Sun C, Wu W, Ma Y, Min T, Lai F, Wu H (2016) Physicochemical, functional properties and antioxidant activities of protein fractions obtained from mulberry (Morus atropurpurea Roxb.) Leaf. Int J Food Prop 20:s3311–s3325CrossRefGoogle Scholar
  34. Timilsena YP, Adhikari R, Barrow CJ, Adhikari B (2016) Physicochemical and functional properties of protein isolate produced from Australian chia seeds. Food Chem 212:648–656CrossRefPubMedGoogle Scholar
  35. Ulloa JA, Rosas-Ulloa P, Ulloa-Rangel BE (2011) Physicochemical and functional properties of a protein isolate produced from safflower (Carthamus tinctorius L.) meal by ultrafiltration. J Sci Food Agric 91:572–577CrossRefPubMedGoogle Scholar
  36. Wells BJ, Rd MA, Everett CJ (2005) Association between dietary arginine and C-reactive protein. Nutrition 21:125–130CrossRefPubMedGoogle Scholar
  37. Yu M, Zeng M, Qin F, He Z, Chen J (2017) Physicochemical and functional properties of protein extracts from Torreya grandis seeds. Food Chem 227:453–460CrossRefPubMedGoogle Scholar
  38. Zhong C, Sun Z, Zhou Z, Jin MJ, Tan ZL, Jia SR (2014) Chemical characterization and nutritional analysis of protein isolates from Caragana korshinskii Kom. J Agric Food Chem 62:3217–3222CrossRefPubMedGoogle Scholar

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© Association of Food Scientists & Technologists (India) 2019

Authors and Affiliations

  1. 1.Institute of Chemical Industry of Forest Products, CAF; National Engineering Lab. for Biomass Chemical Utilization, Key and Open Lab. of Forest Chemical Engineering, SFAKey Lab. of Biomass Energy and MaterialNanjingPeople’s Republic of China
  2. 2.Co-Innovation Center of Efficient Processing and Utilization of Forest ResourcesNanjing Forestry UniversityNanjingPeople’s Republic of China

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