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Volatile profile of fermented sausages with commercial probiotic strains and fructooligosaccharides

  • Camila Vespúcio Bis-Souza
  • Mirian Pateiro
  • Rubén Domínguez
  • José M. Lorenzo
  • Ana Lucia Barretto Penna
  • Andrea Carla da Silva BarrettoEmail author
Original Article
  • 28 Downloads

Abstract

The effect of the partial substitution of pork back fat by fructooligosaccharides (FOS) and the probiotic strains Lactobacillus paracasei and Lactobacillus rhmanosus on the generation of volatile organic compounds in fermented sausages was investigated. The results obtained showed that these factors significantly affected the total content of organic volatile compounds (7484, 8114, 8372 and 10,737 AU × 104/g for FOS.GG, CON, FOS.BGP1 and FOS samples, respectively). A total of 59 volatile components, mainly hydrocarbons, ketones and esters were isolated. The reduction of fat content by including FOS in the formulation results in positive effects and a greater stability of the volatile profile of the fermented sausages, increasing ester compounds and reducing the undesirable notes of hexanal (probiotic samples showed values < 2 AU × 104/g). Moreover, there was a symbiotic effect when the aforementioned prebiotic fiber was combined with probiotic Lactobacillus strains.

Keywords

Meat product Prebiotic FOS Lactic acid bacteria Aroma Volatile compounds 

Notes

Acknowledgements

José M. Lorenzo is member of the Healthy Meat network, funded by CYTED (Ref. 119RT0568). The authors would like to thank the National Council for the Improvement of Higher Education CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) for their financial support.

References

  1. Andrade MJ, Córdoba JJ, Casado EM, Córdoba MG, Rodríguez M (2010) Effect of selected strains of Debaryomyces hansenii on the volatile compound production of dry fermented sausage “salchichón”. Meat Sci 85:256–264CrossRefGoogle Scholar
  2. Ba HV, Seo HW, Seong PN, Kang SM, Kim YS, Cho SH, Park BY, Ham JS, Kim JH (2018) Lactobacillus plantarum (KACC 92189) as a Potential Probiotic Starter Culture for Quality Improvement of Fermented Sausages. Korean J Food Sci Anim Resour 38:189–202Google Scholar
  3. Bis-Souza CV, Henck JMM, Barretto ACS (2018) Performance of low-fat beef burger with added soluble and insoluble dietary fibers. Food Sci Technol 38:522–529CrossRefGoogle Scholar
  4. Bis-Souza CV, Barba FJ, Lorenzo JM, Penna AB, Barretto ACS (2019a) New strategies for the development of innovative fermented meat products: a review regarding the incorporation of probiotics and dietary fibers. Food Rev Int 35:1–18CrossRefGoogle Scholar
  5. Bis-Souza CV, Pateiro M, Domínguez R, Penna ALB, Lorenzo JM, Barretto ACS (2019b) Impact of fructooligosaccharides and probiotic strains on the quality parameters of low-fat Spanish Salchichón. Meat Sci (submitted) Google Scholar
  6. Bosse R, Wirth M, Becker T, Weiss J, Gibis M (2017) Determination of volatile marker compounds in raw ham using headspace-trap gas chromatography. Food Chem 219:249–259CrossRefGoogle Scholar
  7. Cheng JR, Liu XM, Zhang YS (2018) Characterization of Cantonese sausage fermented by a mixed starter culture. J Food Process Pres 42:e13623CrossRefGoogle Scholar
  8. Corral S, Salvador A, Flores M (2013) Salt reduction in slow fermented sausages affects the generation of aroma active compounds. Meat Sci 93:776–785CrossRefGoogle Scholar
  9. De Vrese M, Schrezenmeir J (2008) Probiotics, prebiotics, and synbiotics. In: Stahl U, Donalies UEB, Nevoigt E (eds) Food biotechnology. Springer, Berlin, pp 1–66Google Scholar
  10. Domínguez R, Munekata PE, Agregán R, Lorenzo JM (2016) Effect of commercial starter cultures on free amino acid, biogenic amine and free fatty acid contents in dry-cured foal sausage. LWT-Food Sci Technol 71:47–53CrossRefGoogle Scholar
  11. Domínguez R, Purriños L, Pérez-Santaescolástica C, Pateiro M, Barba FJ, Tomasevic I, Lorenzo JM (2019) Characterization of volatile compounds of dry-cured meat products using HS-SPME-GC/MS technique. Food Anal Methods 12:1263–1284CrossRefGoogle Scholar
  12. FDA (2017) Gras notice (GRN) No. 717 for short-chain fructooligosaccharides (scFOS). Office of Food Additive Safety (FHS-200). Center for Food Safety and Applied Nutrition. Food and Drug Administration, College Park, USAGoogle Scholar
  13. Felisberto MHF, Galvão MTEL, Picone CSF, Cunha RL, Pollonio MAR (2015) Effect of prebiotic ingredients on the rheological properties and microstructure of reduced-sodium and low-fat meat emulsions. LWT-Food Sci Technol 60:148–155CrossRefGoogle Scholar
  14. Fonseca S, Gómez M, Domínguez R, Lorenzo JM (2015) Physicochemical and sensory properties of Celta dry-ripened “salchichón” as affected by fat content. Grasas Aceites 66:059Google Scholar
  15. Gibson GR, Hutkins R, Sanders ME, Prescott SL, Reimer RA, Salminen SJ, Scott K, Stanton C, Swanson KS, Cani PD, Verbeke K, Reid G (2017) Expert consensus document: The International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of prebiotics. Nat Rev Gastroenterol Hepatol 14:491–502CrossRefGoogle Scholar
  16. Gómez M, Lorenzo JM (2013) Effect of fat level on physicochemical, volatile compounds and sensory characteristics of dry-ripened “chorizo” from Celta pig breed. Meat Sci 95:658–666CrossRefGoogle Scholar
  17. Gómez M, Domínguez R, Fonseca S, Lorenzo JM (2015) Effect of finishing diet on physico-chemical and lipolytic parameters and volatile compounds throughout the manufacture of dry-cured foal “Cecina”. Austin J Nutr Food Sci 3:1056Google Scholar
  18. Heck RT, Vendruscolo RG, de Araújo Etchepare M, Cichoski AJ, de Menezes CR, Barin JS, Campagnol PCB (2017) Is it possible to produce a low-fat burger with a healthy n − 6/n − 3 PUFA ratio without affecting the technological and sensory properties? Meat Sci 130:16–25CrossRefGoogle Scholar
  19. Hierro E, de la Hoz L, Ordóñez JA (1997) Contribution of microbial and meat endogenous enzymes to the lipolysis of dry fermented sausages. J Agric Food Chem 45:2989–2995CrossRefGoogle Scholar
  20. Jiménez-Colmenero F, Carballo J, Cofrades S (2001) Healthier meat and meat products: their role as functional foods. Meat Sci 59:5–13CrossRefGoogle Scholar
  21. Kleerebezem M, Boekhorst J, van Kranenburg R, Molenaar D, Kuipers OP, Leer R, Tarchini R, Peters SA, Sandbrink HM, Fiers MW, Stiekema W (2003) Complete genome sequence of Lactobacillus plantarum WCFS1. Proc Natl Acad Sci 100:1990–1995CrossRefGoogle Scholar
  22. Leroy F, Verluyten J, De Vuyst L (2006) Functional meat starter cultures for improved sausage fermentation. Int J Food Microbiol 106:270–285CrossRefGoogle Scholar
  23. Lorenzo JM, Carballo J (2015) Changes in physico-chemical properties and volatile compounds throughout the manufacturing process of dry-cured foal loin. Meat Sci 99:44–51CrossRefGoogle Scholar
  24. Lorenzo JM, Bedia M, Bañón S (2013) Relationship between flavour deterioration and the volatile compound profile of semi-ripened sausage. Meat Sci 93:614–620CrossRefGoogle Scholar
  25. Lorenzo JM, Gómez M, Fonseca S (2014a) Effect of commercial starter cultures on physicochemical characteristics, microbial counts and free fatty acid composition of dry-cured foal sausage. Food Control 46:382–389CrossRefGoogle Scholar
  26. Lorenzo JM, Franco D, Carballo J (2014b) Effect of the inclusion of chestnut in the finishing diet on volatile compounds during the manufacture of dry-cured “Lacón” from Celta pig breed. Meat Sci 96:211–223CrossRefGoogle Scholar
  27. Lorenzo JM, Gómez M, Purriños L, Fonseca S (2016a) Effect of commercial starter cultures on volatile compound profile and sensory characteristics of dry-cured foal sausage. J Sci Food Agric 96:1194–1201CrossRefGoogle Scholar
  28. Lorenzo JM, Munekata PES, Pateiro M, Campagnol PCB, Domínguez R (2016b) Healthy Spanish salchichón enriched with encapsulated n − 3 long chain fatty acids in konjac glucomannan matrix. Food Res Int 89:289–295CrossRefGoogle Scholar
  29. Montanari C, Gatto V, Torriani S, Barbieri F, Bargossi E, Lanciotti R, Grazia L, Magnani R, Tabanelli G, Gardini F (2018) Effects of the diameter on physico-chemical, microbiological and volatile profile in dry fermented sausages produced with two different starter cultures. Food Biosci 22:9–18CrossRefGoogle Scholar
  30. Muriel E, Antequera T, Petron MJ, Andrés AI, Ruiz J (2004) Volatile compounds in Iberian-dry cured loin. Meat Sci 68:391–400CrossRefGoogle Scholar
  31. Narváez-Rivas M, Gallardo E, León-Camacho M (2012) Analysis of volatile compounds from Iberian hams: a review. Grasas Aceites 63:432–454CrossRefGoogle Scholar
  32. Öztürk B, Serdaroğlu M (2017) A rising star prebiotic dietary fiber: inulin and recent applications in meat products. J Food Health Sci 3:12–20Google Scholar
  33. Pastorelli G, Magni S, Rossi R, Pagliarini E, Baldini P, Dirinck P, Van Opstaele F, Corino C (2003) Influence of dietary fat, on fatty acid composition and sensory properties of dry-cured Parma ham. Meat Sci 65:571–580CrossRefGoogle Scholar
  34. Pateiro M, Franco D, Carril JA, Lorenzo JM (2015) Changes on physico-chemical properties, lipid oxidation and volatile compounds during the manufacture of celta dry-cured loin. J Food Sci Technol 52:4808–4818CrossRefGoogle Scholar
  35. Pérez-Santaescolástica C, Carballo J, Fulladosa E, Garcia-Perez JV, Benedito J, Lorenzo JM (2018) Effect of proteolysis index level on instrumental adhesiveness, free amino acids content and volatile compounds profile of dry-cured ham. Food Res Int 107:559–566CrossRefGoogle Scholar
  36. Petričević S, Radovčić NM, Lukić K, Listeš E, Medić H (2018) Differentiation of dry-cured hams from different processing methods by means of volatile compounds, physico-chemical and sensory analysis. Meat Sci 137:217–227CrossRefGoogle Scholar
  37. Purriños L, Franco D, Carballo J, Lorenzo JM (2012) Influence of the salting time on volatile compounds during the manufacture of dry-cured pork shoulder “lacón”. Meat Sci 92:627–634CrossRefGoogle Scholar
  38. Rivas-Cañedo A, Nuñez M, Fernández-García E (2009) Volatile compounds in Spanish dry-fermented sausage ‘salchichón’ subjected to high pressure processing. Effect of the packaging material. Meat Sci 83:620–626CrossRefGoogle Scholar
  39. Rivas-Cañedo A, Juez-Ojeda C, Nuñez M, Fernández-García E (2012) Volatile compounds in low-acid fermented sausage “espetec” and sliced cooked pork shoulder subjected to high pressure processing. A comparison of dynamic headspace and solid-phase microextraction. Food Chem 132:18–26CrossRefGoogle Scholar
  40. Ruiz-Aceituno L, Hernandez-Hernandez O, Kolida S, Moreno FJ, Methven L (2018) Sweetness and sensory properties of commercial and novel oligosaccharides of prebiotic potential. LWT-Food Sci Technol 97:476–482CrossRefGoogle Scholar
  41. Salazar P, García ML, Selgas MD (2009) Short-chain fructooligosaccharides as potential functional ingredient in dry fermented sausages with different fat levels. Int J Food Sci Technol 44:1100–1107CrossRefGoogle Scholar
  42. Sánchez-Peña CM, Luna G, García-González DL, Aparicio R (2005) Characterization of French and Spanish dry-cured hams: influence of the volatiles from the muscles and the subcutaneous fat quantified by SPME-GC. Meat Sci 69:635–645CrossRefGoogle Scholar
  43. Shapiro SS, Wilk MB (1965) An analysis of variance test for normality (complete samples). Biometrika 52:591–611CrossRefGoogle Scholar
  44. Sidira M, Kandylis P, Kanellaki M, Kourkoutas Y (2016) Effect of curing salts and probiotic cultures on the evolution of flavor compounds in dry-fermented sausages during ripening. Food Chem 201:334–338CrossRefGoogle Scholar
  45. Sridevi V, Sumathi V, Guru Prasad M, Kumar MS (2014) Fructooligosaccharides-type prebiotic: a review. J Pharm Res 8:321–330Google Scholar
  46. Sunesen LO, Dorigoni V, Zanardi E, Stahnke L (2001) Volatile compounds released during ripening in Italian dried sausage. Meat Sci 58:93–97CrossRefGoogle Scholar
  47. Toldrá F, Flores M (1998) The role of muscle proteases and lipases in flavor development during the processing of dry-cured ham. Crit Rev Food Sci 38:331–352CrossRefGoogle Scholar
  48. Zhang W, Xiao S, Samaraweera H, Joo Lee E, Ahn DU (2010) Improving functional value of meat products. Meat Sci 86:15–31CrossRefGoogle Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2019

Authors and Affiliations

  1. 1.Department of Food Technology and EngineeringUNESP – São Paulo State UniversitySão José do Rio PretoBrazil
  2. 2.Centro Tecnológico de la Carne de GaliciaRúa Galicia No 4, Parque Tecnológico de GaliciaSan Cibrán das ViñasSpain

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