Chemical composition and biological activity of Mentha citrata Ehrh., essential oils growing in southern Algeria

  • Hamza OuakouakEmail author
  • Naima Benchikha
  • Aicha Hassani
  • Mohamed L. Ashour
Original Article


Three techniques, hydro distillation, steam distillation and microwave-assisted distillation, was used in this work to extract volatile secondary metabolites essential oils (EOs), from the aerial part of Mentha citrata Ehrh., plant (steams, leaves and flowers). The plant material was collected from a location called El-kobna in El-Oued province, southern Algeria during June 2018. The extracted Mentha citrata EOs, were analyzed using both gas chromatography coupled with flame ionization detector and gas chromatography attached with mass spectrometry detector techniques. The antioxidant activity of these EOs were evaluated in vitro using DPPH (2,2-diphenyl-1-picrylhydrazyl) free radicals scavenging activity and cytotoxic test. The results of the EOs analysis showed large variability of the chemical compositions for all techniques. Moreover, a promising cytotoxic activity on colon cancer cells was found.


Essential oil Mentha citrata Ehrh. Lamiaceae Antioxidant Cytotoxic activity GC/MS 



We are very grateful to Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt for providing support and assistance to Hamza Ouakouak. All the authors express sincere grateful thanks to Mr. Mohammed Messaoudi, Research Assistant at Algerian Nuclear Research Centre of Birine (COMENA) for improving the present manuscript. Special thanks to Ms. Eman Ramadan Elsharkawy Professor at Northern Borders University, Saudi Arabia for her help.


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Copyright information

© Association of Food Scientists & Technologists (India) 2019

Authors and Affiliations

  1. 1.Chemistry DepartmentUniversity of Hamma Lakhdar El-OuedEl OuedAlgeria
  2. 2.Laboratoire des produits bioactifs et valorisation de la biomasseEcole normale supérieure de KoubaAlgiesAlgeria
  3. 3.Department of Pharmacognosy, Faculty of PharmacyAin Shams UniversityAbbassia, CairoEgypt
  4. 4.Department of Pharmaceutical Sciences, Pharmacy ProgramBatterjee Medical CollegeNorth Obhur, JeddahSaudi Arabia

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