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Journal of Food Science and Technology

, Volume 56, Issue 12, pp 5194–5203 | Cite as

Synthesis, characterization and cellular mineral absorption of nanoemulsions of Rhododendron arboreum flower extracts stabilized with gum arabic

  • Prince ChawlaEmail author
  • Naveen Kumar
  • Ravinder Kaushik
  • Sanju B. Dhull
Original Article
  • 24 Downloads

Abstract

To assess the cellular mineral uptake and oxidative stability of flower extract, a nanoscale gum arabic stabilized Rhododendron arboreum flower extract emulsion was formulated. Four different concentrations of flower extract (1–5%) were used for the optimization of the nanoemulsion. A significant (P < 0.05) difference was observed in average droplet size (43.51–55.87 nm) of the nanoemulsion. FTIR spectrum confirmed mainly C=C, aliphatic C–H, aliphatic and aromatic galacto-proteins, and polymeric-OH groups present in nanoemulsion. Smooth type of nanoemulsion was confirmed by inverted light microscopy. Ionic strength was evaluated and significant (P < 0.05) increase in particles size was attributed, whereas significant (P < 0.05) decrease in zeta potential was observed by increased NaCl concentration. Iron and calcium showed a non-significant difference in terms of mineral bioavailability. Calcium revealed significantly higher cellular uptake (52.11%) in comparison with iron (50.25%) and zinc (45.32%) during transwell assay. Higher cellular iron uptake unveiled a satisfactory amount of ferritin content

Keywords

Gum arabic Nanoemulsion Rhododendron arboreum TBA Minerals FTIR 

Abbreviations

FE

Flower extract

NE

Nanoemulsion

DMEM

Dulbecco’s modified eagles medium

PBS

Phosphate buffer saline

AAS

Atomic absorbance spectrophotometer

DLS

Dynamic light scattering

FTIR

Fourier transform infrared spectroscopy

TBA

Thiobarbituric acid

Notes

Acknowledgements

The support by School of Bioengineering and Food Technology, Shoolini University, Solan, Himachal Pradesh, India is gratefully acknowledged.

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Copyright information

© Association of Food Scientists & Technologists (India) 2019

Authors and Affiliations

  1. 1.Shoolini UniversitySolanIndia
  2. 2.Amity UniversityJaipurIndia
  3. 3.Chaudhary Devi Lal UniversitySirsaIndia
  4. 4.Lovely Professional UniversityPhagwaraIndia
  5. 5.Amity UniversityNoidaIndia

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