Journal of Food Science and Technology

, Volume 56, Issue 11, pp 5128–5137 | Cite as

Isolation of Enterococcus faecium, characterization of its antimicrobial metabolites and viability in probiotic Minas Frescal cheese

  • Liziane Schittler
  • Luana Martins Perin
  • Juliana de Lima Marques
  • Vanessa Lando
  • Svetoslav Dimitrov Todorov
  • Luís Augusto Nero
  • Wladimir Padilha da SilvaEmail author
Original Article


The aim of this study was to isolate Enterococcus faecium from raw milk samples, to characterize its antimicrobial metabolites, and to evaluate its viability in a probiotic Minas Frescal cheese. For this, antagonist activity against Listeria monocytogenes, safety aspects and biochemical, genotypic, and probiotic characteristics of the isolates were evaluated. Minas Frescal cheese was manufactured with the isolate that showed the best characteristics in vitro, and its viability in the product was evaluated. It was observed that of the 478 lactic acid bacteria isolates, only isolate E297 presented antagonist activity, genes encoding for enterocin production and absence of virulence factors. Besides that, E297 presented probiotic characteristics in vitro, and maintained its viability (8.09 log CFU mL−1) for 14 days of cold storage, when it was added to cheese. Therefore, isolate E297 can be considered a promising microorganism for the manufacture of probiotic foods, especially Minas Frescal cheese.


Bacteriocin-like substances Biopreservation Enterocin LAB Probiotic food 



This study was financed in part by Coordenação de Aperfeiçoamento Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001. The authors would like to thank FAPERGS and CNPq (309101/2016-6) for their financial support for the research.

Compliance with ethical standards

Ethical standards

The article complies with all points of ethical standards.

Supplementary material

13197_2019_3985_MOESM1_ESM.docx (101 kb)
Supplementary material 1 (DOCX 100 kb)


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Copyright information

© Association of Food Scientists & Technologists (India) 2019

Authors and Affiliations

  • Liziane Schittler
    • 1
  • Luana Martins Perin
    • 2
  • Juliana de Lima Marques
    • 3
  • Vanessa Lando
    • 1
  • Svetoslav Dimitrov Todorov
    • 2
  • Luís Augusto Nero
    • 2
  • Wladimir Padilha da Silva
    • 3
    • 4
    Email author
  1. 1.Departamento de Engenharia de AlimentosUniversidade do Estado de Santa CatarinaPinhalzinhoBrazil
  2. 2.Departamento de VeterináriaUniversidade Federal de ViçosaViçosaBrazil
  3. 3.Departamento de Ciência e Tecnologia AgroindustrialUniversidade Federal de PelotasPelotasBrazil
  4. 4.Centro de Desenvolvimento Tecnológico – BiotecnologiaUniversidade Federal de PelotasPelotasBrazil

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