Postharvest biocontrol of Colletotrichum gloeosporioides on mango using the marine bacterium Stenotrophomonas rhizophila and its possible mechanisms of action
- 57 Downloads
The marine bacterium Stenotrophomonas rhizophila was assessed in vitro and in vivo as biocontrol agent against anthracnose disease of mango fruit caused by Colletotrichum gloeosporioides. The results showed that in vitro inhibition of the colony diameter and spore germination of the phytopathogen was due to the production of VOCs, competition for nutrients, and lytic enzymes. When a concentration of 1 × 108 cells ml−1 of the antagonist bacterium was applied to the fruit, disease incidence was reduced by 95%, and the lesion diameter of anthracnose decreased by 85%, which offered greater protection than the synthetic fungicide. This is the first report of antagonistic mechanisms of the marine bacterium S. rhizophila against anthracnose disease in mango, which in this study was found to be more effective than the synthetic fungicide.
KeywordsBiological control Marine bacterium Postharvest disease Stenotrophomonas rhizophila
This work was supported financially by a Grant Project from Problemas Nacionales 2015-01-352 of CONACYT (Consejo Nacional de Ciencia y Tecnología, México). We also acknowledge Ernesto Diaz and R. Galicia for their technical support, and Dr. Michael Cordoba a native English speaking editor for editing the manuscript.
Compliance with ethical standards
Conflict of interest
All authors declare that they have no conflict of interest.
- Afzal I, Shinwari ZK, Iqrar I (2015) Selective isolation and characterization of agriculturally beneficial endophytic bacteria from wild hemp using Canola. Pak J Bot 47:1999–2008Google Scholar
- Bruner LR (1964) Determination of reducing value: 3,5-dinitrosalicylic acid method. In: Whistler RL, Smith RJ, BeMiller JN (eds) Methods in carbohydrate chemistry, vol IV. Academic Press, New York, pp 67–71Google Scholar
- Chandra EP (2017) Microbial volatiles as chemical weapons against pathogenic fungi. In: Choudhary D, Sharma A, Agarwal P, Varma A, Tuteja N (eds) Volatiles and food security. Springer, SingaporeGoogle Scholar
- Egamberdieva D, Jabborova D, Berg G (2016) Synergistic interactions between Bradyrhizobium japonicum and the endophyte Stenotrophomonas rhizophila and their effects on growth, and nodulation of soybean under salt stress. Plant Soil 405:35–45. https://doi.org/10.1007/s11104-015-2661-8 CrossRefGoogle Scholar
- Hernandez-Montiel LG, Gutierrez-Perez ED, Murillo-Amador B, Vero S, Chiquito-Contreras RG, Rincon-Enriquez G (2018) Mechanisms employed by Debaryomyces hansenii in biological control of anthracnose disease on papaya fruit. Postharvest Biol Technol 139:31–37. https://doi.org/10.1016/j.postharvbio.2018.01.015 CrossRefGoogle Scholar
- Yu S, Teng C, Liang J, Song T, Dong L, Bai X, Jin Y, Qu J (2017) Characterization of siderophore produced by Pseudomonas syringae BAF. 1 and its inhibitory effects on spore germination and mycelium morphology of Fusarium oxysporum. J Microbiol 55:877–884. https://doi.org/10.1007/s12275-017-7191-z CrossRefPubMedGoogle Scholar