Journal of Food Science and Technology

, Volume 56, Issue 11, pp 4795–4798 | Cite as

Determination of 17β-estradiol in commercial pasteurized and sterilized milk samples in Mashhad, Iran

  • Bamdad Riahi-Zanjani
  • Mostafa Heidarzadegan
  • Hasan Badibostan
  • Gholamreza KarimiEmail author
Original Article


Due to nutritional facts of milk in human life, the quality assessment of dairy products is of the utmost importance. The aim of the current study was to determine the 17β-estradiol level in commercial pasteurized and sterilized milk brands in Mashhad, Iran. In this regard, 160 samples including 80 pasteurized (40 high-fat and 40 low-fat) and 80 sterilized milk (40 high-fat and 40 low-fat) of widely used brands from different supermarkets were collected. The mean level of 17β-estradiol was 8.2 ± 0.59 pg/ml. The mean amount of estradiol was found to be 7.6 ± 0.47, 7.9 ± 0.45, 8.6 ± 0.63, and 8.9 ± 0.54 pg/ml for the low-fat pasteurized, low-fat sterilized, high-fat pasteurized and high-fat sterilized milk, respectively. There was no significant difference between the amount of estradiol in pasteurized and sterilized milk. As expected, the level of estradiol was statistically higher in high fat milks than that of low-fat milks. Considering the levels of 17β-estradiol measured here and the maximum permissible daily level of external estradiol entered to body through edible products recommended by EU and CAC (3.5 µg), at least in the short term, there will be no remarkable impact on the endocrine system. However, judging the long-term effects of using these products is not easy and simple at all, as cancers develop during a long period of time and has a multifactorial etiology.


17β-Estradiol Pasteurized milk Sterilized milk 



The authors are thankful to the Vice Chancellor of Research, Mashhad University of Medical Sciences (Grand No. 941661) for financial support.

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest.


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Copyright information

© Association of Food Scientists & Technologists (India) 2019

Authors and Affiliations

  • Bamdad Riahi-Zanjani
    • 1
  • Mostafa Heidarzadegan
    • 2
  • Hasan Badibostan
    • 2
  • Gholamreza Karimi
    • 2
    • 3
    Email author
  1. 1.Medical Toxicology Research CenterMashhad University of Medical SciencesMashhadIran
  2. 2.Department of Pharmacodynamics and Toxicology, School of PharmacyMashhad University of Medical SciencesMashhadIran
  3. 3.Pharmaceutical Research Center, Institute of Pharmaceutical TechnologyMashhad University of Medical SciencesMashhadIran

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