Profile of chlorophylls and carotenoids of wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) microgreens
The use of cereal microgreens is increasing because of increased consumer’s interest in healthier products. Chlorophyll (Chl) and Carotenoids (Car) are suggested to correlate with health promoting components like phenolics and antioxidant potential of the plant-part. They also play role against clinical conditions like thalassemia and hemolytic anemia and reduce the risk of some chronic diseases, such as cancer, cardiovascular diseases, skin diseases and age-related eye diseases. This study was carried out for the comprehensive profiling of Chl and Car in wheat (Triticum aestivum L.), and barley (Hordeum vulgare L.) micro-greens between 7 and 16 days on dry basis. Chl and Car content strongly correlated with the number of days of growth. Significantly high correlations existed among Chl a, Chl b, total Chl and total Car with concomitant Chl a/b and Chl/Car ratios. The peaks for the rate of accumulation of pigments were between 7–10 days on wheat and 10–13 days on barley. The maximum content of Chl and Car were 616.63 ± 18.45 mg/100 g DM and 54.80 ± 1.72 mg/100 g DM on day 16 and statistically not significant to variety of grain. The Chl level was slightly lower than Chl rich vegetables like kale and comparable to spinach and Car level was comparable to carrots, which is higher than most of the daily consumable fruits and vegetables. Further cell-based or in vivo studies of cereal microgreens could be considered to draw more valuable information related to human health.
KeywordsChlorophyll Carotenoids Germination Microgreens Wheat Barley
Authors would like to thank National College of Food Science and Technology (NCFST), Tribhuvan University, Nepal for the Internal grant.
- Eldahshan OA, Singab ANB (2013) Carotenoids. J Pharmacogn Phytochem 2:225–234Google Scholar
- Filimon RV, Rotaru L, Filimon RM (2016) Quantitative investigation of leaf photosynthetic pigments during annual biological cycle of Vitis vinifera L. table grape cultivars. S Afr J Enol Vitic 37:1–14Google Scholar
- Kohler GO (1944) The effect of stage of growth on the chemistry of the grasses. J Biol Chem 152:215–223Google Scholar
- Meng FL, Xv YY, Hu JD, He D, Jiang JH (2017) Optimization of combined drying process by hot-air and microwave for barley seedling powder. J Food Saf Qual 8:1651–1658Google Scholar
- Ndukwe OK, Edeoga HO, Okwulehie IC, Omosun G (2016) Variability in the Chlorophyll and Carotene Composition of ten maize (Zea mays) varieties. Eur J Phys Agric Sci 4:1–6Google Scholar
- Padalia S, Drabu S, Raheja I, Gupta A, Dhamija M (2010) Multitude potential of wheatgrass juice (Green Blood): an overview. Chron Young Sci 1:23–28Google Scholar
- Simova-stoilova L, Stoyanova Z, Demirevska-kepova K (2001) Ontogenic changes in leaf pigments, total soluble proteins and Rubisco in two barley varieties in relation to yield. Bulgerian J Plant Physiol 27:15–24Google Scholar
- Skoczylas L, Korus A, Tabaszewska M, Gedos K, Szczepanska E (2018) Evaluation of the quality of fresh and frozen wheatgrass juices depending on the time of grass harvest. J Food Process Preserv 43:1–8Google Scholar
- Wakeham P (2013) The medicinal and pharmacological screening of wheatgrass juice (Triticum aestivum L.): an investigation into chlorophyll content and antimicrobial activity. Plymouth Stud Sci 6:20–30Google Scholar
- Yang CM, Lee CN, Chou CH (2002) Effects of three allelopathic phenolics on chlorophyll accumulation of rice (Oryza sativa) seedlings: I. Inhibition of supply-orientation. Bot Bull Academia Sinica 43:299–324Google Scholar
- Zaripheh S, Erdman JW (2002) Factors that influence the bioavailablity of xanthophylls. In: Can lutein protect against chronic disease? pp S531–S534Google Scholar