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Journal of Food Science and Technology

, Volume 56, Issue 4, pp 2267–2277 | Cite as

Physicochemical and microbiological characteristics of honey obtained through sugar feeding of bees

  • Md. Mostafa Kamal
  • Md. Harun Ur Rashid
  • Shakti Chandra Mondal
  • Hasan Fuad El TajEmail author
  • Chuleui Jung
Original Article
  • 121 Downloads

Abstract

This study was planned to characterize the physicochemical and antioxidant properties, and microbiological quality of honey obtained from the sandbar pumpkin field. In this study, four sugar supplemented and one control (without sugar fed) honey sample was used. Results revealed that all samples exhibited appropriate maturity considering their low moisture content (~ 19%) and high total solids (~ 80%) and TSS (~ 79%). Total acidity (< 40 meq/kg) and pH (~ 4.5) directed the absenteeism of detrimental fermentation. Ash (~ 0.29%) and electrical conductivity (~ 700 µS/cm) were reasonable and distinctive of dark yellowish-brown honey, which is buttressed by color attributes. Reducing sugars, glucose, fructose, and sucrose values ranged from 68.98 to 75.82%, 26.01 to 33.84%, 34.93 to 38.70%, and 1.74 to 5.96%, respectively. Proline (~ 400 mg/kg), HMF (< 40 mg/kg) and diastase action (~ 14° Gothe) were found within accepted limits, and also possesses good antioxidants in terms of total phenol (~ 160 mg GAE/100 g), total flavonoid (4.67–6.25 mg CE/100 g), and DPPH-RSA (30.65–35.97%). The microbial study revealed that the total viable count ranged between 33.33 and 27.66 CFU/g, while yeasts and mold count varied between 14.33 and 12 CFU/g. Principle component analysis (PCA) results revealed that all the studied parameters could be used effectively to discriminate the honey sample. The overall results signpost a new information regarding the quality i.e. processing, maturity, freshness and composition of honey obtained from the sandbar pumpkin field.

Keywords

Honey Sugar feeding Physicochemical Microbiological PCA Sandbar cropping 

Notes

Acknowledgement

We thankfully acknowledged the help of the beekeepers for kind supplying of honey samples, Dr. Maruf Ahmed for providing DPPH reagent and helping in PCA analysis, and Md. Saifullah, PhD Fellow, School of Environmental and Life Sciences, University of Newcastle, Central Coast, Ourimbah, NSW 2258, Australia for proofreading the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Al ML, Daniel D, Moise A et al (2009) Physico-chemical and bioactive properties of different floral origin honeys from Romania. Food Chem 112:863–867CrossRefGoogle Scholar
  2. AOAC (1990) Official methods of analysis. Association of Official Agricultural Chemists, Washington, DCGoogle Scholar
  3. Azonwade FE, Paraiso A, Dossa CPA et al (2018) Physicochemical characteristics and microbiological quality of honey produced in Benin. J Food Qual 2018:1–13CrossRefGoogle Scholar
  4. Babarinde GO, Babarinde SA, Adegbola DO, Ajayeoba SI (2011) Effects of harvesting methods on physicochemical and microbial qualities of honey. J Food Sci Technol 48:628–634CrossRefGoogle Scholar
  5. Baroni MV, Arrua C, Nores ML et al (2009) Composition of honey from Córdoba (Argentina): assessment of north/south provenance by chemometrics. Food Chem 114:727–733CrossRefGoogle Scholar
  6. Bath PK, Singh N (1999) A comparison between Helianthus annuus and Eucalyptus lanceolatus honey. Food Chem 67:389–397CrossRefGoogle Scholar
  7. Bertoncelj J, Doberšek U, Jamnik M, Golob T (2007) Evaluation of the phenolic content, antioxidant activity and colour of Slovenian honey. Food Chem 105:822–828CrossRefGoogle Scholar
  8. Buba F, Gidado A, Sgugaba A (2013) Physicochemical and microbiological properties of honey from North East Nigeria. Biochem Anal Biochem 2:1–7CrossRefGoogle Scholar
  9. Can Z, Yildiz O, Sahin H et al (2015) An investigation of Turkish honeys: their physico-chemical properties, antioxidant capacities and phenolic profiles. Food Chem 180:133–141CrossRefGoogle Scholar
  10. Codex Alimentarius Commission (1981) Revised codex standard for honey Codex Stan 12-1981, Rev. 1 (1987), Rev. 2 (2001). Codex Standard 12:1–7Google Scholar
  11. de Sousa JMB, de Souza EL, Marques G et al (2016) Sugar profile, physicochemical and sensory aspects of monofloral honeys produced by different stingless bee species in Brazilian semi-arid region. LWT Food Sci Technol 65:645–651CrossRefGoogle Scholar
  12. Downey SG, Hussey K, Daniel Kelly J et al (2005) Preliminary contribution to the characterisation of artisanal honey produced on the island of Ireland by palynological and physico-chemical data. Food Chem 91:347–354CrossRefGoogle Scholar
  13. European Comission (2002) European Commission Council Directive 2001/110/EC of 20 December 2001 relating to honey. Off J Eur Communities 10:47Google Scholar
  14. García-Martínez E, Igual M, Martín-Esparza ME, Martínez-Navarrete N (2013) Assessment of the bioactive compounds, color, and mechanical properties of apricots as affected by drying treatment. Food Bioprocess Technol 6:3247–3255CrossRefGoogle Scholar
  15. Gomes S, Dias LG, Moreira LL et al (2010) Physicochemical, microbiological and antimicrobial properties of commercial honeys from Portugal. Food Chem Toxicol 48:544–548CrossRefGoogle Scholar
  16. Imtara H, Elamine Y, Lyoussi B (2018) Physicochemical characterization and antioxidant activity of Palestinian honey samples. Food Sci Nutr 6(8):2056–2065CrossRefGoogle Scholar
  17. International Honey Commission (2009) Harmonized methods of the international honey commission. Retrieved from http://www.ihc-platform.net/ihcmethods2009.pdf
  18. Khalil MI, Alam N, Moniruzzaman M et al (2011) Phenolic acid composition and antioxidant properties of Malaysian honeys. J Food Sci 76:921–928CrossRefGoogle Scholar
  19. Khalil MI, Moniruzzaman M, Boukraâ L et al (2012) Physicochemical and antioxidant properties of algerian honey. Molecules 17:11199–11215CrossRefGoogle Scholar
  20. Kim DO, Jeong SW, Lee CY (2003) Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chem 81:321–326CrossRefGoogle Scholar
  21. Kirs E, Pall R, Martverk K, Laos K (2011) Physicochemical and melissopalynological characterization of Estonian summer honeys. Procedia Food Sci 1:616–624CrossRefGoogle Scholar
  22. Küçük M, Kolayli S, Karaoǧlu Ş et al (2007) Biological activities and chemical composition of three honeys of different types from Anatolia. Food Chem 100:526–534CrossRefGoogle Scholar
  23. Meda A, Lamien CE, Romito M et al (2005) Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food Chem 91:571–577CrossRefGoogle Scholar
  24. Nayik GA, Nanda V (2016) A chemometric approach to evaluate the phenolic compounds, antioxidant activity and mineral content of different unifloral honey types from Kashmir, India. LWT—Food Sci Technol 74:504–513Google Scholar
  25. Nayik GA, Dar BN, Nanda V (2016) Physico-chemical, rheological and sugar profile of different unifloral honeys from Kashmir valley of India. Arab J Chem.  https://doi.org/10.1016/j.arabjc.2015.08.017 Google Scholar
  26. Nayik GA, Suhag Y, Majid I, Nanda V (2018) Discrimination of high altitude Indian honey by chemometric approach according to their antioxidant properties and macro minerals. J Saudi Soc Agric Sci 17:200–207Google Scholar
  27. Ouchemoukh S, Louaileche H, Schweitzer P (2007) Physicochemical characteristics and pollen spectrum of some Algerian honeys. Food Control 18:52–58CrossRefGoogle Scholar
  28. Rashid MH, El Taj HF, Chowdhury NI et al (2018) Supplement feeding to honeybee colony for field crop pollination, pumpkin and honey production in sandbar cropping system. J Apic 33:25–32CrossRefGoogle Scholar
  29. Sahinler N, Sahinler S, Gul A (2004) Biochemical composition of honeys produced in Turkey. J Apic Res 43:53–56CrossRefGoogle Scholar
  30. Saxena S, Gautam S, Sharma A (2010) Physical, biochemical and antioxidant properties of some Indian honeys. Food Chem 118:391–397CrossRefGoogle Scholar
  31. Silva TMS, dos Santos FP, Evangelista-Rodrigues A et al (2013) Phenolic compounds, melissopalynological, physicochemical analysis and antioxidant activity of jandaíra (Melipona subnitida) honey. J Food Compos Anal 29:10–18CrossRefGoogle Scholar
  32. Singh N, Bath PK (1997) Quality evaluation of different types of Indian honey. Food Chem 58:129–133CrossRefGoogle Scholar
  33. Singh I, Singh S (2018) Honey moisture reduction and its quality. J Food Sci Technol 55:3861–3871CrossRefGoogle Scholar
  34. Sousa JMB, De Souza AI, Magnani M et al (2013) Aspectos físico-químicos e perfil sensorial de méis de abelhas sem ferrão da região do Seridó, Estado do Rio Grande do Norte, Brasil. Semin Agrar 34:1765–1774Google Scholar
  35. Souza BA, Roubik D, Heard T et al (2006) Composition of stingless bee honey: setting quality standards. Interciencia 31:867–875Google Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2019

Authors and Affiliations

  • Md. Mostafa Kamal
    • 1
  • Md. Harun Ur Rashid
    • 2
  • Shakti Chandra Mondal
    • 1
  • Hasan Fuad El Taj
    • 2
    Email author
  • Chuleui Jung
    • 3
  1. 1.Department of Food Processing and PreservationHajee Mohammad Danesh Science and Technology UniversityDinajpurBangladesh
  2. 2.Department of EntomologyHajee Mohammad Danesh Science and Technology UniversityDinajpurBangladesh
  3. 3.School of Bioresource, Graduate SchoolAndong National UniversityAndongRepublic of South Korea

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