Journal of Food Science and Technology

, Volume 56, Issue 4, pp 2326–2331 | Cite as

Physical adsorption of patulin by Saccharomyces cerevisiae during fermentation

  • Zhuo Zhang
  • Min Li
  • Caie Wu
  • Bangzhu PengEmail author
Short Communication


Patulin (PAT), a mycotoxin mainly produced by various species of fungi, is frequently detected in moldy fruit- and vegetable-based products, which pose a health risk to the consumer. Over the past decades, a few studies reported that PAT content could be significantly decreased by microbial fermentation process. However, the physical adsorption mechanism between PAT and yeast during fermentation is still unclear. In this paper, we focused on the physical adsorption of PAT by Saccharomyces cerevisiae CCTCC 93161 during fermentation in aqueous solutions. Firstly, morphology of differently treated yeast cells were analyzed by scanning electron microscope, then the interactions between PAT and yeast cells were investigated by infrared absorption spectra of differently treated S. Cerevisiae cells before and after the adsorption of PAT. The results showed that the efficiency of PAT removal raised significantly with the increase of fermentation temperature and time, whereas it decreased significantly with the increase of initial PAT concentration in the fermentation system. The proteins and polysaccharides in the cell walls of yeast interacted with PAT and accounted for the physical adsorption. The current work would possibly provide some new insights on PAT control for fermented foods.


Patulin Saccharomyces cerevisiae Fermentation Physical adsorption 



This research was partially supported by the National Natural Science Foundation of China (No. 31671850) and Natural Science Foundation of Hubei Province of China (No. 2017CFB599).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. Du J, Guo C (2016) Adsorption of patulin from apple juice by waste beer yeast. Food Sci 37(5):56–61 (In Chinese) Google Scholar
  2. Ge N, Xu J, Li F, Peng B, Pan S (2017) Immobilization of inactivated microbial cells on magnetic Fe3O4@CTS nanoparticles for constructing a new biosorbent for removal of patulin in fruit juice. Food Control 82:83–90CrossRefGoogle Scholar
  3. Guo C, Yue T, Hatab S, Yuan Y (2012) Ability of inactivated yeast powder to adsorb patulin from apple juice. J Food Protect 75:585–590CrossRefGoogle Scholar
  4. Harwig J, Scott PM, Kennedy BPC, Chen YK (1973) Disappearance of patulin from apple juice fermented by Saccharomyces spp. Can I Food Sci Tech J 6(1):45–46CrossRefGoogle Scholar
  5. Kannamba B, Reddy KL, AppaRao BV (2010) Removal of Cu(II) from aqueous solutions using chemically modified chitosan. J Hazard Mater 175:939–948CrossRefGoogle Scholar
  6. Li M, Chen W, Zhang Z, Zhang Z, Peng B (2018) Fermentative degradation of patulin by saccharomyces cerevisiae in aqueous solution. LWT Food Sci Tech 97:427–433CrossRefGoogle Scholar
  7. Liu B, Peng X, Meng X (2018) Effective biodegradation of mycotoxin patulin by porcine pancreatic lipase. Front Microbiol 9:615CrossRefGoogle Scholar
  8. Luo Y, Wang J, Liu B, Wang Z, Yuan Y, Yue T (2015) Effect of yeast cell morphology, cell wall physical structure and chemical composition on patulin adsorption. PLoS one 10(8):e0136045CrossRefGoogle Scholar
  9. Moss MO, Long MT (2002) Fate of patulin in the presence of the yeast Saccharomyces cerevisiae. Food Addit Contam A 19:387–399CrossRefGoogle Scholar
  10. Neri F, Donati I, Veronesi F, Mazzoni D, Mari M (2010) Evaluation of Penicillium expansum isolates for aggressiveness growth and patulin accumulation in usual and less common fruit hosts. Food Microbiol 143:109–117CrossRefGoogle Scholar
  11. Oteiza JM, Khaneghah AM, Campagnollo FB, Granato D, Mahmoudi MR, Sant’Ana AS (2017) Influence of production on the presence of patulin and ochratoxin A in fruit juices and wines of Argentina. LWT Food Sci Tech 80:200–207CrossRefGoogle Scholar
  12. Ricelli A, Baruzzi F, Solfrizzo M, Morea M, Fanizzi FP (2007) Biotransformation of patulin by Glucono bacteroxydans. Appl Environ Microbiol 73:785–792CrossRefGoogle Scholar
  13. Richard JL (2007) Some major mycotoxins and their mycotoxicoses—an overview. Int J Food Microbiol 119(1–2):3–10CrossRefGoogle Scholar
  14. Tannous J, Snini SP, El KR, Canlet C, Pinton P, Lippi Y (2017) Patulin transformation products and last intermediates in its biosynthetic pathway, E- and Z ascladiol, are not toxic to human cells. Arch Toxicol 9:2455–2467CrossRefGoogle Scholar
  15. Wang L, Yue T, Yuan Y, Wang Z, Ye M, Cai R (2015) A new insight into the adsorption mechanism of patulin by the heat-inactive lactic acid bacteria cells. Food Control 50:104–110CrossRefGoogle Scholar
  16. Yuan Y, Wang X, Hatab S, Wang Z, Wang Y, Luo Y (2014) Patulin reduction in apple juice by inactivated Alicyclobacillus spp. Lett Appl Microbiol 59:604–609CrossRefGoogle Scholar
  17. Yue T, Dong Q, Guo C, Worobo RW (2011) Reducing patulin contamination in apple juice by using inactive yeast. J Food Prot 74:149–153CrossRefGoogle Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2019

Authors and Affiliations

  1. 1.Key Laboratory of Environment Correlative Dietology, Ministry of Education, College of Food Science & TechnologyHuazhong Agricultural UniversityWuhanChina
  2. 2.College of Light Industry and Food EngineeringNanjing Forestry UniversityNanjingChina

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