Journal of Food Science and Technology

, Volume 56, Issue 4, pp 2016–2026 | Cite as

Isolation, identification and application on soy sauce fermentation flavor bacteria of CS1.03

  • Xuewei JiangEmail author
  • Yantao Xu
  • Jing Ye
  • Zijiang Yang
  • Shouen Huang
  • Yongle Liu
  • Shangting Zhou
Original Article


Bacillus is a common bacterial genus in Chinese traditional sun-brewed moromi. Some Bacillus species can metabolize sauce flavour and aroma substances. To address the insufficiency of flavour microbes in soy sauce fermentation, we used 16S rDNA sequence analysis to identify the CS1.03 strain, which was isolated from moromi produced through the traditional and natural sun brewing methods. A Box–Behnken design was used to optimize the addition technology of CS1.03, and ammoniacal nitrogen content and sensory evaluation were adopted as determination indices. GC–MS analysis was also performed to analyse the flavour components. Results identified CS1.03 as Bacillus subtilis. The optimal conditions yielded 10.7 g/L ammoniacal nitrogen in fermented soy sauce with rich aroma and flavour, and a sensory evaluation score of 90.5. GC–MS analysis revealed that the contents of phenol, aldehyde and ketone are all increased. These results demonstrated that CS1.03 could improve the flavour of soy sauce and shows good potential for application in soy sauce fermentation.


Flavour microorganism Soy sauce fermentation 16S rDNA sequence analysis Bacillus subtilis Box–Behnken design GC–MS analysis 



This work was supported by Hunan Provincial Natural Science Foundation of China, analysis of brewing microorganism and construction of dominant flora in soy sauce with high-salt liquid state technology (No. 2018JJ2421) and the Foundation Project of Changsha Science and Technology Program, research and demonstration of key technology in soy sauce production with multi-strain fermentation (No. kq1601013). There are no copyright disputes. We thank Liwen Bianji, Edanz Group China (, for editing the English text of a draft of this manuscript.

Compliance with ethical standards

Conflict of interest

The author declares that they have no conflict of interest.

Supplementary material

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Copyright information

© Association of Food Scientists & Technologists (India) 2019

Authors and Affiliations

  1. 1.School of Chemistry and Food EngineeringChangsha University of Science and TechnologyChangshaChina
  2. 2.Hunan Provincial Engineering Technology Research Center for Condiment FermentationChangshaChina
  3. 3.Jiajia Food Group Co. Ltd.ChangshaChina

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