Sustainable approach for lycopene extraction from tomato processing by-product using hydrophobic eutectic solvents

  • Yasmini P. A. Silva
  • Tânia A. P. C. Ferreira
  • Guangling Jiao
  • Marianne S. BrooksEmail author
Short Communication


Lycopene, a non-polar antioxidant compound with important effects on human health and wide commercial applications, was extracted from tomato processing wastes using innovative hydrophobic eutectic mixtures (HEMs) replacing traditional organic solvents. HEMs were prepared using DL-menthol as hydrogen-bond acceptor (HBA) and lactic acid as hydrogen-bond donor (HBD), and the ultrasound-assisted extraction (UAE) was optimized using a Box–Behnken design to evaluate extraction conditions: extraction temperature (°C), molar ratio of eutectic mixture (moles HBA: mol HBD), solvent to sample ratio (volume to mass, mL/g), and extraction time (min), with lycopene extraction yield (µg/g d.w.) as the response variable. Optimization of parameters was performed using response surface methodology, and the optimized extraction conditions were determined to be 70 °C, 8:1 mol HBA/mol HBD, 120 mL/g solvent: sample, and 10 min. The experimental optimal yield was 1446.6 µg/g, in agreement with the predicted optimal yield, indicating the validity of the model. This new technique for lycopene extraction, using a HEM as extraction solvent in replacement of hazardous organic solvents, and tomato pomace as source material, represents a viable and more sustainable approach for obtaining a high value-added bioactive compound, and can contribute towards the development of greener extraction processes.


Green extraction Deep eutectic solvents Tomato pomace Response surface methodology Box–Behnken design 



The authors would like to thank the processing company who provided the material used in this study. The authors acknowledge the Coordination for the Improvement of Higher Education Personnel (CAPES—Brazil), the Department of Foreign Affairs, Trade and Development (DFATD—Canada), and the Natural Sciences and Engineering Research Council (NSERC—Canada) for financial support. The funding sources had no involvement in the design of the research study, data collection/analysis, writing of the report, or submission of the article for publication.

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest.


  1. Abbott AP, Boothby D, Capper G, Davies DL, Rasheed RK (2004) Deep eutectic solvents formed between choline chloride and carboxylic acids: versatile alternatives to ionic liquids. J Am Chem Soc 126:9142–9147CrossRefGoogle Scholar
  2. Berger PD, Maurer RE, Celli GB (2018) Experimental design with applications in management, engineering, and the sciences, 2nd edn. Springer International Publishing, ChamGoogle Scholar
  3. Box GEP, Hunter JS, Hunter WG (2005) Statistics for experimenters: design, innovation, and discovery, 2nd edn. Wiley-Interscience, HobokenGoogle Scholar
  4. Brittton G (1995) UV/visible spectroscopy. In: Britton G, Liaaen-Jensen S, Pfander H (eds) Carotenoids. Volume 1B. Spectroscopy. Birhäuser Verlag, Basel, pp 13–62Google Scholar
  5. Calvo MM, Dado D, Santa-Maria G (2007) Influence of extraction with ethanol or ethyl acetate on the yield of lycopene, β-carotene, phytoene and phytofluene from tomato peel powder. Eur Food Res Technol 224:567–571CrossRefGoogle Scholar
  6. Cao J, Yang M, Cao F, Wang J, Su E (2017a) Tailor-made hydrophobic deep eutectic solvents for cleaner extraction of polyprenyl acetates from Ginkgo biloba leaves. J Clean Prod 152:399–405CrossRefGoogle Scholar
  7. Cao J, Yang M, Cao F, Wang J, Su E (2017b) Well-designed hydrophobic deep eutectic solvents as green and efficient media for the extraction of artemisinin from Artemisia annua leaves. ACS Sustain Chem Eng 5:3270–3278CrossRefGoogle Scholar
  8. Chemat F, Vian MA, Cravotto G (2012) Green extraction of natural products: concept and principles. Int J Mol Sci 13:8615–8627CrossRefGoogle Scholar
  9. Chemat F, Rombaut N, Sicaire A, Meullemiestre A, Fabiano-Tixier A, Abert-Vian M (2017) Ultrasound assisted extraction of food and natural products. Mechanisms, techniques, combinations, protocols and applications. A review. Ultrason Sonochem 34:540–560CrossRefGoogle Scholar
  10. Chen J, Liu M, Wang Q, Du H, Zhang L (2016) Deep eutectic solvent-based microwave-assisted method for extraction of hydrophilic and hydrophobic components from Radix Salviae miltiorrhizae. Molecules 21:1383CrossRefGoogle Scholar
  11. Dai Y, van Spronsen J, Witkamp G, Verpoorte R, Choi YH (2013) Natural deep eutectic solvents as new potential media for green technology. Anal Chim Acta 766:61–68CrossRefGoogle Scholar
  12. Duan L, Dou L, Guo L, Li P, Liu E (2016) Comprehensive evaluation of deep eutectic solvents in extraction of bioactive natural products. ACS Sustain Chem Eng 4:2405–2411CrossRefGoogle Scholar
  13. Espino M, Fernández MLA, Gomez FJV, Silva MF (2016) Natural designer solvents for greening analytical chemistry. TRAC Trend Anal Chem 76:126–136CrossRefGoogle Scholar
  14. Henry LK, Catignani GL, Schwartz SJ (1998) Oxidative degradation kinetics of lycopene, lutein, and 9-cis and all-trans β-carotene. J Am Oil Chem Soc 75:823–829CrossRefGoogle Scholar
  15. Li X, Row KH (2016) Development of deep eutectic solvents applied in extraction and separation. J Sep Sci 39:3505–3520CrossRefGoogle Scholar
  16. Rao AV, Agarwal S (1999) Role of lycopene as antioxidant carotenoid in the prevention of chronic diseases: a review. Nutr Res 19:305–323CrossRefGoogle Scholar
  17. Ribeiro BD, Florindo C, Iff LC, Coelho MAZ, Marrucho IM (2015) Menthol-based eutectic mixtures: hydrophobic low viscosity solvents. ACS Sustain Chem Eng 3:2469–2477CrossRefGoogle Scholar
  18. Ruesgas-Ramón M, Figueroa-Espinoza MC, Durand E (2017) Application of deep eutectic solvents (DES) for phenolic compounds extraction: overview, challenges, and opportunities. J Agric Food Chem 65:3591–3601CrossRefGoogle Scholar
  19. Silva YPA, Ferreira TAPC, Celli GB, Brooks MS (2018) Optimization of lycopene extraction from tomato processing waste using an eco-friendly ethyl lactate-ethyl acetate solvent—a green valorization approach. Waste Biomass Valori. Google Scholar
  20. Strati IF, Oreopoulou V (2011) Effect of extraction parameters on the carotenoid recovery from tomato waste. Int J Food Sci Technol 46:23–29CrossRefGoogle Scholar
  21. Strati IF, Oreopoulou VI (2014) Recovery of carotenoids from tomato processing by-products—a review. Food Res Int 65:311–321CrossRefGoogle Scholar
  22. Suslick KS (1990) Sonochemistry. Science 247:1439–1445CrossRefGoogle Scholar
  23. Van Osch DJGP, Zubeir LF, van den Bruinhorst A, Rocha MAA, Kroon MC (2015) Hydrophobic deep eutectic solvents as water-immiscible extractants. Green Chem 17:4518–4521CrossRefGoogle Scholar
  24. WPTC—World Processing Tomato Council (2016) World production estimate. Accessed 12 April 2016
  25. Zainal-Abidin MH, Hayyan M, Hayyan A, Jayakumar NS (2017) New horizons in the extraction of bioactive compounds using deep eutectic solvents: a review. Anal Chim Acta 979:1–23CrossRefGoogle Scholar
  26. Zhang Q, Vigier KO, Royer S, Jérôme F (2012) Deep eutectic solvents: syntheses, properties and applications. Chem Soc Rev 41:7108–7146CrossRefGoogle Scholar
  27. Zhang H, Tang B, Row KH (2014) A green deep eutectic solvent-based ultrasound-assisted method to extract astaxanthin from shrimp byproducts. Anal Lett 47:742–749CrossRefGoogle Scholar
  28. Zhang Y, Li Z, Wang W, Xuan X, Wang J (2016) Efficient separation of phenolic compounds from model oil by the formation of choline derivative-based deep eutectic solvents. Sep Purif Technol 163:310–318CrossRefGoogle Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2019

Authors and Affiliations

  • Yasmini P. A. Silva
    • 1
  • Tânia A. P. C. Ferreira
    • 1
  • Guangling Jiao
    • 2
  • Marianne S. Brooks
    • 2
    Email author
  1. 1.Faculty of NutritionFederal University of GoiásGoiâniaBrazil
  2. 2.Department of Process Engineering and Applied ScienceDalhousie UniversityHalifaxCanada

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