Bioactivities of Nizamuddinia zanardinii sulfated polysaccharides extracted by enzyme, ultrasound and enzyme-ultrasound methods

  • Mehdi Alboofetileh
  • Masoud RezaeiEmail author
  • Mehdi Tabarsa
  • SangGuan You
Original Article


Sulfated polysaccharide (fucoidan) was isolated from Nizamuddinia zanardinii by enzyme (alcalase), ultrasonic and enzyme-ultrasonic methods. The extracted fucoidans were assessed for their chemical compositions, molecular characteristics, anticancer and immunomodulatory activities. Enzyme-ultrasonic isolated fucoidan showed the maximum extraction yield (7.87%) while that obtained by ultrasonic had the minimum value (3.6%). fucoidans were composed of different levels of carbohydrates (52.78–58.65%), proteins (6.98–8.91%), sulfates (21.78–29.6%) and uronic acids (0.42–1.08%). The weight mean average molecular weight of fucoidans varied between 443.7 and 1020.85 kDa. The polysaccharide chains were consisted of fucose, galactose, glucose, mannose and xylose. All the recovered fucoidans showed strong growth inhibition against HeLa and Hep-G2 cancer cells. The isolated fucoidans were non-toxic and considerably stimulated the macrophage cells to release nitric oxide. Enzyme extraction produced fucoidan with the most macrophage stimulation capacity (> 42 µmol). These results suggested that enzyme treatment preserved more sulfate groups in fucoidan structure influencing its anticancer and immunostimulatory activities.


Nizamuddinia zanardinii Fucoidan Enzyme extraction Molecular weight Sulfate group 



Authors would like to thank the Iran National Science Foundation (INSF) for support of this research.


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Copyright information

© Association of Food Scientists & Technologists (India) 2019

Authors and Affiliations

  1. 1.Department of Seafood Processing, Faculty of Marine SciencesTarbiat Modares UniversityNoorIran
  2. 2.Department of Marine Food Science and TechnologyGangneung-Wonju National UniversityGangneungRepublic of Korea

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