Effect of starter culture on microbiological, physiochemical and nutrition quality of Xiangxi sausage

  • Sha Du
  • Huan Cheng
  • Jin-Kui Ma
  • Zong-jun Li
  • Chuan-hua WangEmail author
  • Yuan-Liang WangEmail author
Original Article


Fermented sausages have a long tradition originating from China. In this study, three starter microorganisms including Pediococcus pentosaceus (P), Staphylococcus xylosus (S), and a combination of P. pentosaceus and S. xylosus (P + S) were conducted for the manufacture of traditional Xiangxi (a city in China) fermented sausages. The physicochemical changes of the above three kinds of sausages during fermentation were studied and discussed, and also compared with these properties on the natural fermented sausage (N, i.e., control). The results revealed that five kinds of bacterial phases were existed at different fermentation stages in N, P, S and P + S fermented sausages, respectively. The microbiological data showed that an initial enterobacteria count of approximately 5.3 log CFU/g for all four batches of sausages. The enterobacteria count in the inoculated sausages of P and P + S groups decreased significantly to about 1 log CFU/g whereas group N and S had a count of about 3.3 log CFU/g after fermentation. In the early stages of fermentation, the pH rapidly decreased below 5.3. FAA and FFA were significantly increased in all groups and TBARS value in group P was higher than that of the other three groups. In conclusion, starter cultures can be used to improve the hygiene level of Xiangxi sausages without significant effects on pH, AW, and nitrite residue.


Xiangxi sausage Pedicoccus pentosaceus Staphylococcus xylosus Microorganism and physicochemical characteristics Free amino acid Free fatty acid 



Natural fermentation


Pediococcus pentosaceus


Staphylococcus xylosus

P + S

Mixed fermentation


Free amino acid


Free fatty acid


Thiobarbituric acid reactive substances


Mono-unsaturated fatty acids


Polyunsaturated fatty acids



Authors are grateful for financial supports from the Department of Agriculture of Hunan Province, China, and Core Research Program 1515, Hunan Agricultural University.


  1. Alicia O, José Luis N, Mónica F (2011) Effect of fat content on aroma generation during processing of dry fermented sausages. Meat Sci 87(3):264–273. CrossRefGoogle Scholar
  2. Arief II, Wulandari Z, Aditia EL et al (2014) Physicochemical and microbiological properties of fermented lamb sausages using probiotic Lactobacillus plantarum, IIA-2C12 as starter culture. Procedia Environ Sci 20:352–356. CrossRefGoogle Scholar
  3. Candogan K, Wardlaw FB, Acton JC (2009) Effect of starter culture on proteolytic changes during processing of fermented beef sausages. Food Chem 116(3):731–737. CrossRefGoogle Scholar
  4. Casquete R, Benito MJ, Martín A et al (2011) Role of an autochthonous starter culture and the protease EPg222 on the sensory and safety properties of a traditional Iberian dry-fermented sausage “salchichón”. Food Microbiol 28(8):1432–1440. CrossRefPubMedGoogle Scholar
  5. Dawood MAO, Koshio S (2016) Recent advances in the role of probiotics and prebiotics in carp aquaculture: a review. Aquaculture 454(28):243–251. CrossRefGoogle Scholar
  6. Essid I, Hassouna M (2013) Effect of inoculation of selected Staphylococcus xylosus, and Lactobacillus plantarum, strains on biochemical, microbiological and textural characteristics of a Tunisian dry fermented sausage. Food Control 32(2):707–714. CrossRefGoogle Scholar
  7. Flores M, Corral S, Cano-García L et al (2015) Yeast strains as potential aroma enhancers in dry fermented sausages. Int J Food Microbiol 212:16–24. CrossRefPubMedGoogle Scholar
  8. Gonzales-Barron U, Cadavez V, Pereira AP et al (2015) Relating physicochemical and microbiological safety indicators during processing of linguiça, a Portuguese traditional dry-fermented sausage. Food Res Int 78:50–61. CrossRefPubMedGoogle Scholar
  9. Gøtterup J, Olsen K, Knøchel S et al (2008) Colour formation in fermented sausages by meat-associated staphylococci with different nitrite- and nitrate-reductase activities. Meat Sci 78(4):492–501. CrossRefPubMedGoogle Scholar
  10. Hierro E, de la Hoz LHL, Ordóñez JA (1999) Contribution of the microbial and meat endogenous enzymes to the free amino acid and amine contents of dry fermented sausages. J Agric Food Chem 47(3):1156–1161. CrossRefPubMedGoogle Scholar
  11. Jofré A, Aymerich T, Garriga M (2015) Probiotic fermented sausages: myth or reality? Procedia Food Sci 5:133–136. CrossRefGoogle Scholar
  12. Juan AO, Eva MH, Jose MB et al (1999) Changes in the components of dry-fermented sausages during ripening. Crit Rev Food Sci Nutr 39(4):329–367. CrossRefGoogle Scholar
  13. Kozačinski L, Drosinos E, Čaklovica F, Cocolin L, Gasparikreichardt J, Vesković S (2008) Investigation of microbial association of traditionally fermented sausages. Food Technol Biotechnol 46(1):93–106Google Scholar
  14. Lorenzo JM, Gómez M, Fonseca S (2014) Effect of commercial starter cultures on physicochemical characteristics, microbial counts and free fatty acid composition of dry-cured foal sausage. Food Control 46:382–389. CrossRefGoogle Scholar
  15. Muguerza E, Gimeno O, Ansorena D et al (2004) New formulations for healthier dry fermented sausages: a review. Trends Food Sci Technol 15(9):452–457. CrossRefGoogle Scholar
  16. Nie X, Lin S, Zhang Q (2014) Proteolytic characterisation in grass carp sausage inoculated with Lactobacillus plantarum, and Pediococcus pentosaceus. Food Chem 145(7):840–844. CrossRefPubMedGoogle Scholar
  17. Olivares A, Navarro JL, Salvador A, Flores M (2010) Sensory acceptability of slow fermented sausages based on fat content and ripening time. Meat Sci 86(2):251–257. CrossRefPubMedGoogle Scholar
  18. Rantsiou K, Cocolin L (2008) Fermented meat products. Food Microbiol Food Saf 27(94):91–118CrossRefGoogle Scholar
  19. Rantsiou K, Urso R, Iacumin L, Cantoni C, Cattaneo P, Comi G, Cocolin L (2005) Culture-dependent and -independent methods to investigate the microbial ecology of Italian fermented sausages. Appl Environ Microbiol 71(4):1977–1986. CrossRefPubMedPubMedCentralGoogle Scholar
  20. Ravyts F, Steen L, Goemaere O et al (2010) The application of staphylococci with flavour-generating potential is affected by acidification in fermented dry sausages. Food Microbiol 27(7):945–954. CrossRefPubMedGoogle Scholar
  21. Santiago RM, Alberto M, María José B et al (2011) Application of Lactobacillus fermentum HL57 and Pediococcus acidilactici SP979 as potential probiotics in the manufacture of traditional Iberian dry-fermented sausages. Food Microbiol 28(5):839–847. CrossRefGoogle Scholar
  22. Simion AMC, Vizireanu C, Alexe P et al (2014) Effect of the use of selected starter cultures on some quality, safety and sensorial properties of Dacia sausage, a traditional Romanian dry-sausage variety. Food Control 35(1):123–131. CrossRefGoogle Scholar
  23. Wang XH, Ren HY, Liu DY et al (2013) Effects of inoculating Lactobacillus sakei, starter cultures on the microbiological quality and nitrite depletion of Chinese fermented sausages. Food Control 32(2):591–596. CrossRefGoogle Scholar
  24. Zhang Y, Wang C (2010) Research on fermented meat products of domestic progress. Jiangxi Food Ind 2:46–48Google Scholar
  25. Zhao L, Jin Y, Ma C et al (2011) Physico-chemical characteristics and free fatty acid composition of dry fermented mutton sausages as affected by the use of various combinations of starter cultures and spices. Meat Sci 88(4):761–766. CrossRefPubMedGoogle Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2019

Authors and Affiliations

  1. 1.College of Food Science and TechnologyHunan Agricultural UniversityChangshaChina
  2. 2.College of Chemistry and Chemical EngineeringZhaoqing UniversityZhaoqingChina

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